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Lee, Kim, and Chi: Saccharomyces boulardii Reduced Intestinal Inflammation in Mice Model of 2,4,6-trinitrobencene Sulfonic Acid Induced Colitis: Based on Microarray
Original Article

Korean J Gastroenterol 2010;55(1):33-45.

Published online: 21 February 2010

DOI: https://doi.org/10.4166/kjg.2010.55.1.33

Saccharomyces boulardii Reduced Intestinal Inflammation in Mice Model of 2,4,6-trinitrobencene Sulfonic Acid Induced Colitis: Based on Microarray

Sang Kil Lee, M.D., Hyo Jong Kim, M.D.∗, Sung Gil Chi, Ph.D.†

Department of Internal Medicine, Institute of Gastroenterology, Yonsei University College of Medicine, Seoul, Korea

∗Department of Internal Medicine, School of Medicine, Kyung Hee University, Seoul, Korea

†School of Life Sciences and Biotechnology, Korea University, Seoul, Korea

Correspondence to: Sang Kil Lee, M.D. Department of Internal Medicine, Yonsei University College of Medicine, 250, Seongsanno, Seodaemun-gu, Seoul 120-752, Korea Tel: +82-2-2228-1991, Fax: +82-2-393-6884 E-mail: sklee@yuhs.ac

Received 5 February 2009       Accepted 29 July 2009

Copyright © 2010 The Korean Journal of Gastroenterology

Abstract

Background/Aims

Saccharomyces boulardii has been reported to be beneficial in the treatment of inflammatory bowel disease. The aim of this work was to evaluate the effect of S. boulardii in a mice model of 2,4,6-trini-trobencene sulfonic acid (TNBS) induced colitis and analyze the expression of genes in S. boulardii treated mice by microarray.

Methods

BALB/c mice received TNBS or TNBS and S. boulardii treatment for 4 days. Microarray was performed on total mRNA form colon, and histologic evaluation was also performed.

Results

In mice treated with S. boulardii, the histological appearance and mortality rate were significantly restored compared with rats receiving only TNBS. Among 330 genes which were altered by both S. boulardii and TNBS (＞2 folds), 193 genes were down-regulated by S. boulardii in microarray. Most of genes which were down-regulated by S. bouardii were functionally classified as inflammatory and immune response related genes.

Conclusions

S. boulardii may reduce colonic inflammation along with regulation of inflammatory and immune responsive genes in TNBS-induced colitis.

Keywords: Saccharomyces boulardii, Microarray, TNBS colitis

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Fig. 1.
The effect of S. boulardii on the histological features of TNBS-induced colitis. Representative photographs of colon sections were shown after treatment with vehicle (A), TNBS (B), and TNBS with S. boulardii (C) (H&E staining, ×40). Histological scoring is shown by graph (D). Results are the mean±SD from five rats per group (∗p＜0.05). SB, S. boulardii.
kjg-55-33f1.tif
Fig. 2.
Functional classification of differentially expressed genes on treatment of Saccharomyces boulardii.
kjg-55-33f2.tif
Table 1.
Down-regulated Genes Modulations by S. boulardii on TNBS-induced Colitis Mice
Probe ID Gene symbol Gene name Folds
mCG8652.2 BB219290 Expressed sequence BB219290 23.927
mCG65051.3 Tgtp T-cell specific GTPase 22.2054
mCG15918.2 Ltb Lymphotoxin B 21.3349
mCG12531.2 Cxcl10 Chemokine (C-X-C motif) ligand 10 20.4804
mCG12528.2 Cxcl9 Chemokine (C-X-C motif) ligand 9 18.5716
mCG22168.2 Cd53 CD53 antigen 14.7447
mCG23449.2 Cd37 CD37 antigen 14.6591
mCG7050.1 Thrsp Thyroid hormone responsive SPOT14 homolog (Rattus) 14.6573
mCG17997.2 Indo Indoleamine-pyrrole 2,3 dioxygenase 14.3727
mCG146987 Gbp1 Guanylate nucleotide binding protein 1 14.1406
mCG2198.2 Il2rg Interleukin 2 receptor, gamma chain 13.9665
mCG12963.1 Cd52 CD52 antigen 13.1077
mCG4163.1 Pou2af1 POU domain, class 2, associating factor 1 12.7336
mCG128171.1 Samsn1 SAM domain, SH3 domain and nuclear localisation signals, 1 12.5214
mCG22405.1 Coro1a Coronin, actin binding protein 1A 12.3893
mCG20328.2 5830443L24Rik RIKEN cDNA 5830443L24 gene 11.4283
mCG13343.2 Rac2 RAS-related C3 botulinum substrate 2 11.2916
mCG3876.1 Ptprcap Protein tyrosine phosphatase, receptor type, C polypeptide-associated protein 11.1825
mCG124701.3 11.0969
mCG132703.2 H2-DMb2 Histocompatibility 2, class II, locus Mb2 10.7731
mCG15086.3 9.63236
mCG12169.2 0610039P13Rik RIKEN cDNA 0610039P13 gene 9.17402
mCG140488 Upp1 Uridine phosphorylase 1 8.91284
mCG19184 Grem1 Gremlin 1 8.84209
mCG13376.2 6330406L22Rik RIKEN cDNA 6330406L22 gene 8.65699
mCG7082.2 Arhgdib Rho, GDP dissociation inhibitor (GDI) beta 8.18446
mCG117393.1 Serpina1e; Serine (or cysteine) proteinase inhibitor, clade A, member 1e;serine 8.11905
Serpina1d; (or cysteine) proteinase inhibitor, clade A, member 1d;serine (or cysteine)
Serpina1b proteinase inhibitor, clade A, member 1b
mCG128030.1 Ian4 Immune associated nucleotide 4 8.00182
mCG3870.3 1810062O14Rik RIKEN cDNA 1810062O14 gene 7.97324
mCG131283 7.94101
mCG8259.2 Il16 Interleukin 16 7.90424
mCG6027.2 Ii Ia-associated invariant chain 7.57815
mCG113208.1 H2-Aa Histocompatibility 2, class II antigen A, alpha 7.50726
mCG133140.1 Gnai2 Guanine nucleotide binding protein, alpha inhibiting 2 7.40195
mCG141811 7.26196
mCG128047.2 Ian1 Immune associated nucleotide 1 7.19016
mCG4614.2 Cnot7 CCR4-NOT transcription complex, subunit 7 7.1873
mCG13056.2 Wars Tryptophanyl-tRNA synthetase 7.17602
mCG1828.1 Edg1 Endothelial differentiation sphingolipid G-protein-coupled receptor 1 7.17183
mCG145061 Myo1g Myosin IG 6.99996
mCG127161.1 6.99374
mCG128050.2 Ian6 Immune associated nucleotide 6 6.97217
NM_007534.1 Bcl2a1d; B-cell leukemia/lymphoma 2 related protein A1d;B-cell leukemia/lymphoma 6.96582
Bcl2a1b;Bcl2a1a 2 related protein A1b;B-cell leukemia/lymphoma 2 related protein A1a
mCG142489 E430029F06 Hypothetical protein E430029F06 6.91118
mCG117402 6.57967
mCG128236.1 Hcls1 Hematopoietic cell specific Lyn substrate 1 6.47773
NM_011335.1 Ccl21a; Chemokine (C-C motif) ligand 21a (leucine);chemokine (C-C motif) ligand 6.43251
Ccl21c; Ccl21b 21c (leucine);chemokine (C-C motif) ligand 21b (serine)
mCG9941.2 H2-Ea Histocompatibility 2, class II antigen E alpha 6.35982
mCG122229 Gbp2 Guanylate nucleotide binding protein 2 6.33139
mCG126565.1 Snn Stannin 6.25258
mCG21119.2 Gbp3 Guanylate nucleotide binding protein 3 6.17898
mCG20688.2 Igtp Interferon gamma induced GTPase 6.15952
mCG17400.2 Pik3cd Phosphatidylinositol 3-kinase catalytic delta polypeptide 6.11848
mCG131749.1 Scd1 Stearoyl-Coenzyme A desaturase 1 6.07188
mCG126030.1 5.96361
mCG10585.2 Lcp1 Lymphocyte cytosolic protein 1 5.90658
mCG132597.1 Slc28a2 Solute carrier family 28 (sodium-coupled nucleoside transporter), member 2 5.87419
NM_011335.1 Ccl21a; Chemokine (C-C motif) ligand 21c (leucine);chemokine (C-C motif) ligand 5.85052
Ccl21c;Ccl21b 21b (serine);chemokine (C-C motif) ligand 21a (leucine)
mCG5445.2 Slpi Secretory leukocyte protease inhibitor 5.83702
mCG23058.2 2210407C18Rik RIKEN cDNA 2210407C18 gene 5.77665
mCG147553 5.76224
mCG12906.1 5.72635
NM_008218.1 Hba-a1 Hemoglobin alpha, adult chain 1 5.70473
mCG16534.2 Rnase6 Ribonuclease A family, member 6 5.6391
AK080028.1 5.62519
mCG128455.1 Klhl6 Kelch-like 6 (Drosophila) 5.5446
mCG3553.3 5.52214
mCG1551.2 Cd3g CD3 antigen, gamma polypeptide 5.5062
mCG9784.2 Rbp1 Retinol binding protein 1, cellular 5.39273
mCG9940.2 H2-Eb1 Histocompatibility 2, class II antigen E beta 5.38968
mCG10007.2 Cd48 CD48 antigen 5.27933
mCG10007.2 Cd48 CD48 antigen 5.27933
mCG1036474.1 Gm457 Gene model 457, (NCBI) 5.23029
mCG132704.1 H2-Ab1;Rmcs1 Histocompatibility 2, class II antigen A, beta 1;response to metastatic 5.18907
cancers 1
mCG23208.1 Fscn1 Fascin homolog 1, actin bundling protein (Strongylocentrotus purpuratus) 5.02444
mCG142733 Ebi2 Epstein-Barr virus induced gene 2 4.95872
mCG9639.2 Lmo2 LIM domain only 2 4.75237
mCG5817.2 6330442E10Rik RIKEN cDNA 6330442E10 gene 4.63981
mCG11329.2 Prg Proteoglycan, secretory granule 4.52879
mCG2785.1 Ly6e Lymphocyte antigen 6 complex, locus E 4.48894
mCG22224.1 S100a14 S100 calcium binding protein A14 4.48858
mCG147775 4.38969
mCG7804.1 Cd2 CD2 antigen 4.38821
mCG119015.1 Art2a ADP-ribosyltransferase 2a 4.36866
mCG3383.2 Itgb2 Integrin beta 2 4.35904
mCG147992 4.3208
mCG14500.2 Rgl1 Ral guanine nucleotide dissociation stimulator,-like 1 4.29709
mCG14500.2 Rgl1 Ral guanine nucleotide dissociation stimulator,-like 1 4.29709
mCG122231 4.28879
mCG21986.3 Samhd1 SAM domain and HD domain, 1 4.27603
mCG23016.2 H2-DMa Histocompatibility 2, class II, locus DMa 4.24347
mCG23012.2 Tap1 Transporter 1, ATP-binding cassette, subfamily B (MDR/TAP) 4.23619
mCG12901.1 Ms4a6b Membrane-spanning 4-domains, subfamily A, member 6B 4.23418
mCG23392.1 Ubd Ubiquitin D 4.17595
NM_175219.2 4930565N07Rik; RIKEN cDNA 4930565N07 gene; RIKEN cDNA A530032D15Rik gene 4.17036
A530032D15Rik
mCG12686.2 Cyfip2 Cytoplasmic FMR1 interacting protein 2 4.14345
mCG119015.1 Art2a ADP-ribosyltransferase 2a 4.12792
mCG123521.1 4.0905
mCG12243.1 Lck Lymphocyte protein tyrosine kinase 4.08069
mCG131454.1 Serpina1a Serine (or cysteine) proteinase inhibitor, clade A, member 1a 3.89904
mCG130733.1 Mef2c Myocyte enhancer factor 2C 3.837
mCG130733.1 Mef2c Myocyte enhancer factor 2C 3.837
mCG124992 9230117N10Rik RIKEN cDNA 9230117N10 gene 3.81886
mCG1025534.1 3.77988
mCG127143.3 Bin1 Bridging integrator 1 3.73025
mCG68209.2 Ly6a Lymphocyte antigen 6 complex, locus A 3.69634
mCG50405 Gpr10 G protein-coupled receptor 10 3.68147
mCG128035.2 Imap38 Immunity-associated protein 3.67731
mCG21468.1 Ifit2 Interferon-induced protein with tetratricopeptide repeats 2 3.66858
mCG3160.2 Nin Ninein 3.65906
mCG4723.2 D1Ertd578e DNA segment, Chr 1, ERATO Doi 578, expressed 3.64958
AK033032.1 3.63308
mCG21922.2 BC065791 cDNA sequence BC065791 3.61352
mCG16735.2 L259 L259 3.57373
mCG13158.1 Mfng Manic fringe homolog (Drosophila) 3.56692
mCG18182.2 Satb1 Special AT-rich sequence binding protein 1 3.54207
mCG7943.2 Wsb2 WD repeat and SOCS box-containing 2 3.53116
mCG6766.2 Il18bp Interleukin 18 binding protein 3.52036
AK079036.1 3.50756
mCG126571.1 Socs1 Suppressor of cytokine signaling 1 3.49201
mCG10374.1 Ushbp1 Usher syndrome 1C binding protein 1 3.48985
mCG142594 AW539457 Expressed sequence AW539457 3.47479
mCG1037964.1 3.47235
mCG3070.2 Prkcb Protein kinase C, beta 3.46484
mCG3250.1 Bgn Biglycan 3.44171
AK048574.1 3.42587
mCG3224.1 Ube2l6 Ubiquitin-conjugating enzyme E2L 6 3.41934
mCG18018.1 Serpine1 Serine (or cysteine) proteinase inhibitor, clade E, member 1 3.34988
mCG1939.1 Slc15a3 Solute carrier family 15, member 3 3.32076
mCG14415.3 Ifi47 Interferon gamma inducible protein 3.30643
mCG20689.3 AI481100 Expressed sequence AI481100 3.30637
mCG13352.1 Pscd4 Pleckstrin homology, Sec7 and coiled/coil domains 4 3.26798
mCG8601.2 Trim30 Tripartite motif protein 30 3.23656
mCG133006.1 Cxcl12 Chemokine (C-X-C motif) ligand 12 3.22899
mCG119756.1 Evi2a;Evi2b Ecotropic viral integration site 2a;ecotropic viral integration site 2b 3.2026
mCG13425.2 Cnn2 Calponin 2 3.18133
mCG16148.2 B130017P16Rik RIKEN cDNA B130017P16 gene 3.17662
mCG23011.3 Psmb9 Proteosome (prosome, macropain) subunit, beta type 9 (large multifunctional protease 2) 3.15542
mCG15923.1 Aif1 Allograft inflammatory factor 1 3.14676
mCG115375.1 Rin3 Ras and Rab interactor 3 3.13998
mCG140647.1 Ebf1 Early B-cell factor 1 3.10564
mCG141865 Il10ra Interleukin 10 receptor, alpha 3.09147
mCG120042.2 Ifi203 Interferon activated gene 203 3.07131
mCG67998.3 BC028265 cDNA sequence BC028265 3.06844
mCG15413.2 AI132321 Expressed sequence AI132321 3.04168
mCG21536.1 Rgs19 Regulator of G-protein signaling 19 2.99075
mCG9634.1 Msc Musculin 2.93674
mCG134497 Arhgap9 Rho GTPase activating protein 9 2.92521
mCG13049.2 Evl Ena-vasodilator stimulated phosphoprotein 2.90988
mCG14532.1 Pla2g2a Phospholipase A2, group IIA (platelets, synovial fluid) 2.81785
mCG123683.2 Stat1 Signal transducer and activator of transcription 1 2.81546
mCG19410.2 Ifi1 Interferon inducible protein 1 2.79991
mCG19429.2 Cd83 CD83 antigen 2.78891
mCG14574.2 Abcg1 ATP-binding cassette, subfamily G (WHITE), member 1 2.7282
mCG17340.2 Arhgef6 Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6 2.72696
mCG10394.2 1110021O17Rik RIKEN cDNA 1110021O17 gene 2.67625
mCG11761.2 Sipa1 Signal-induced proliferation associated gene 1 2.67308
AK039433.1 2.6576
mCG9128.2 Irf5 Interferon regulatory factor 5 2.61609
mCG23014.2 Psmb8 Proteosome (prosome, macropain) subunit, beta type 8 2.59473
(large multifunctional protease 7)
mCG6301.2 Mfge8 Milk fat globule-EGF factor 8 protein 2.59275
mCG8601.2 Trim30 Tripartite motif protein 30 2.56618
mCG10245.2 Chst2 Carbohydrate sulfotransferase 2 2.50992
NM_021342.1 Kcne4 Potassium voltage-gated channel, Isk-related subfamily, gene 4 2.45661
mCG18089.1 9930117H01Rik RIKEN cDNA 9930117H01 gene 2.38466
AK040125.1 2.38273
mCG16847.1 Ela1 Elastase 1, pancreatic 2.37377
mCG1036442.1 2.36078
mCG15331.2 Pscdbp Pleckstrin homology, Sec7 and coiled-coil domains, binding protein 2.35156
mCG1044012.1 2.33769
mCG127250 2.33122
mCG2520.2 Msn Moesin 2.32181
mCG118897.1 2.30729
mCG22120.1 2.29228
mCG131858 Malt1 Mucosa associated lymphoid tissue lymphoma translocation gene 1 2.27686
mCG3993.2 Fignl1 Fidgetin-like 1 2.26777
mCG127246 2.26028
mCG1036441.1 2.25944
mCG127079 Plcg2 Phospholipase C, gamma 2 2.25731
mCG131876.1 2.21653
mCG131871.1 2.20543
AK037888.1 2.2002
mCG142175.1 2.15129
mCG1322.2 2310015N21Rik RIKEN cDNA 2310015N21 gene 2.14179
mCG120176 Xdh Xanthine dehydrogenase 2.13901
mCG148075 Pirb Paired-Ig-like receptor B 2.12779
mCG16780.2 1200002N14Rik RIKEN cDNA 1200002N14 gene 2.12405
mCG121924.1 BC024988 cDNA sequence BC024988 2.09799
mCG121990.2 2.08857
mCG13347.2 Ncf4 Neutrophil cytosolic factor 4 2.03751
mCG13763.2 Zbp1 Z-DNA binding protein 1 2.00081
mCG142177 −2.2672
mCG8493.1 Cxcl13 Chemokine (C-X-C motif) ligand 13 4.7651
Table 2.
Up-regulated Genes Modulations by S. boulardii on TNBS-induced Colitis Mice
Probe ID Gene symbol Gene name Folds
mCG14995.1 14.3012
mCG144713 12.3179
mCG1048584.1 10.307
mCG22595.2 Cyp2e1 Cytochrome P450, family 2, subfamily e, polypeptide 1 9.12296
mCG23624.2 5.91482
mCG10187.2 Hp Haptoglobin 5.28022
mCG17008.1 9130214H05Rik RIKEN cDNA 9130214H05 gene 4.38685
mCG1036745.1 Clra C lectin-related protein A 4.09383
mCG3025.2 Mal Myelin and lymphocyte protein, T-cell differentiation protein 3.50259
mCG17572.1 Igfbp6 Insulin-like growth factor binding protein 6 3.15539
mCG23533.1 2700055K07Rik RIKEN cDNA 2700055K07 gene 3.04113
mCG18681.2 Suox Sulfite oxidase 3.00239
mCG126985.1 Ceacam1 CEA-related cell adhesion molecule 1 2.78879
mCG64633.2 1600014C10Rik RIKEN cDNA 1600014C10 gene 2.73547
mCG23648.1 2.66288
mCG14105.2 Slc30a1 Solute carrier family 30 (zinc transporter), member 1 2.49302
mCG13846.2 2.42384
mCG18465.3 Rgs1 Regulator of G-protein signaling 1 2.35598
mCG123049.1 2.33164
mCG5749.2 Tgfb3 Transforming growth factor, beta 3 2.32376
mCG13037.1 Papss2 3'-phosphoadenosine 5'-phosphosulfate synthase 2 2.22205
mCG128612.1 Retnlb Resistin like beta 2.18414
mCG15002.2 Pcsk1 Proprotein convertase subtilisin/kexin type 1 2.03469
mCG144019 Saa2 Serum amyloid A 2 −2.1437
mCG10001.1 Cyp2c40 Cytochrome P450, family 2, subfamily c, polypeptide 40 −2.1528
mCG8136.1 Cyp2d9 Cytochrome P450, family 2, subfamily d, polypeptide 9 −2.4565
mCG2287.1 Spon1 Spondin 1, (f-spondin) extracellular matrix protein −2.499
AK020017.1 −2.5372
mCG22749.2 Cyp2f2 Cytochrome P450, family 2, subfamily f, polypeptide 2 −2.5767
mCG127939.2 Siat10 Sialyltransferase 10 (alpha-2,3-sialyltransferase VI) −2.6004
mCG56771.1 V2r14;V2r15 Vomeronasal 2, receptor, 14; vomeronasal 2, receptor, 15 −2.7693
mCG11079.2 Syt8 Synaptotagmin 8 −2.7721
mCG14330.2 Vmd2l1 Vitelliform macular dystrophy 2-like protein 1 −2.7847
mCG2257.2 −2.8908
mCG133073.1 Cyp2d10 Cytochrome P450, family 2, subfamily d, polypeptide 10 −2.9862
mCG13564.2 Clic6 Chloride intracellular channel 6 −3.3309
mCG147772 Prss22 Protease, serine, 22 −3.3795
mCG144019 Saa2 Serum amyloid A 2 −3.4
mCG19608.1 Cldn14 Claudin 14 −3.4074
mCG2145.1 Cbr2 Carbonyl reductase 2 −3.5264
mCG141343.1 Olfr78 Olfactory receptor 78 −3.6151
mCG141087 Lbp Lipopolysaccharide binding protein −4.3871
mCG142167 −4.4803
mCG120735.1 Clca2 Chloride channel calcium activated 2 −4.6123
NM_146058.1 BC025446 cDNA sequence BC025446 −4.8128
mCG113451.2 Gyk Glycerol kinase −5.9278
mCG145386 −7.8678
mCG140410 −8.892
mCG16663.2 −18.215
mCG12625.1 C530030I18Rik RIKEN cDNA C530030I18 gene −2.0197
mCG9091.2 Akr1c6 Aldo-keto reductase family 1, member C6 −2.0207
AK036268.1 −2.0449
AK033575.1 −2.0471
mCG131372.1 C730031G17 Hypothetical protein C730031G17 −2.0605
mCG4123.1 Slc37a2 Solute carrier family 37 (glycerol-3-phosphate transporter), member 2 −2.0646
mCG20217.1 1110036H21Rik RIKEN cDNA 1110036H21 gene −2.071
mCG128542 1810030J14Rik RIKEN cDNA 1810030J14 gene −2.0728
mCG14936.2 Ankrd9 Ankyrin repeat domain 9 −2.1062
mCG18385.3 Prph1 Peripherin 1 −2.1267
mCG14756.1 Sult1c1 Sulfotransferase family, cytosolic, 1C, member 1 −2.1515
mCG19644.1 9530008L14Rik RIKEN cDNA 9530008L14 gene −2.1565
mCG21373.1 Nags N-acetylglutamate synthase −2.1886
mCG16530 Ang2 Angiogenin, ribonuclease A family, member 2 −2.2097
mCG114463.1 −2.2176
mCG9562.2 B230106I24Rik RIKEN cDNA B230106I24 gene −2.2318
mCG1036820.1 −2.2359
mCG1097.3 Pcp4 Purkinje cell protein 4 −2.2432
mCG19913.2 Hao3 Hydroxyacid oxidase (glycolate oxidase) 3 −2.261
mCG20217.1 1110036H21Rik RIKEN cDNA 1110036H21 gene −2.2621
mCG125080.1 Sst Somatostatin −2.286
mCG16684.2 Slc22a1 Solute carrier family 22 (organic cation transporter), member 1 −2.295
mCG129806.1 Myh11 Myosin heavy chain 11, smooth muscle −2.3381
mCG130231.1 Cyp2c37 Cytochrome P450, family 2. subfamily c, polypeptide 37 −2.3582
mCG10065.2 Snurf;Snrpn SNRPN upstream reading frame;small nuclear ribonucleoprotein N −2.3624
AK013860.1 −2.4178
mCG11432.1 Abhd6 Abhydrolase domain containing 6 −2.4302
mCG9319.1 Aldh1a7 Aldehyde dehydrogenase family 1, subfamily A7 −2.4433
mCG4878.1 Ckm Creatine kinase, muscle −2.4598
mCG128542 1810030J14Rik RIKEN cDNA 1810030J14 gene −2.5076
mCG6071.2 Akr1b7 Aldo-keto reductase family 1, member B7 −2.5138
mCG21459.2 0610041G09Rik; RIKEN cDNA 0610041G09 gene;actin, alpha 2, smooth muscle, aorta −2.5255
Acta2
mCG129677.1 C030022K24Rik RIKEN cDNA C030022K24 gene −2.5297
mCG1122.2 Mab21l2 Mab-21-like 2 (C. elegans) −2.6082
mCG123117.1 MGC68340 Unknown (protein for MGC:68340) −2.6544
mCG1049272.1 −2.784
mCG16519.2 Ang1;Rnase4 Angiogenin, ribonuclease A family, member 1;ribonuclease, RNase A −2.8052
family 4
mCG6867.2 Cyp3a13 Cytochrome P450, family 3, subfamily a, polypeptide 13 −2.9001
mCG14318.3 Ugt1a1;Ugt1a9; UDP-glucuronosyltransferase 1 family, member 1; −2.9614
Ugt1a12;Ugt1a5; UDP glycosyltransferase 1 family polypeptide A9;
Ugt1a10;Ugt1a13; UDP glycosyltransferase 1 family polypeptide A12;
Ugt1a6;Ugt1a2 UDP glycosyltransferase 1 family, polypeptide A5;
UDP glycosyltransferase 1 family, polypeptide A10;
UDP glycosyltransferase 1 family polypeptide A13;
UDP glycosyltransferase 1 family, polypeptide A6;
UDP-glucuronosyltransferase 1 family, member 2
mCG119907.1 −3.0104
mCG3350.2 Clca1 Chloride channel calcium activated 1 −3.1356
mCG16440.2 Ostb Organic solute transporter beta −3.1435
mCG20604.2 9030617O03Rik RIKEN cDNA 9030617O03 gene −3.1734
mCG1028722 −3.2555
mCG7009.1 Iapp Islet amyloid polypeptide −3.2712
mCG20388.3 Cyp2d22 Cytochrome P450, family 2, subfamily d, polypeptide 22 −3.317
mCG141179.1 Eif2c4 Eukaryotic translation initiation factor 2C, 4 −3.3875
mCG123507.2 Nap1l5 Nucleosome assembly protein 1-like 5 −3.3916
mCG147547 −3.4183
mCG20507.1 −3.4401
mCG6008.2 Ggt1 Gamma-glutamyltransferase 1 −3.4753
mCG20406.2 Tfpi2 Tissue factor pathway inhibitor 2 −3.5075
mCG123117.1 MGC68340 Unknown (protein for MGC:68340) −3.5204
mCG23713.3 Cyp4f14 Cytochrome P450, family 4, subfamily f, polypeptide 14 −3.5671
mCG142287 Slc26a3 Solute carrier family 26, member 3 −3.5847
BC050987.1 −3.6021
mCG18656.1 Tlr5 Toll-like receptor 5 −3.617
mCG13591.2 F830003B07 Hypothetical protein F830003B07 −3.6674
mCG147785 Saa3 Serum amyloid A 3 −3.7447
mCG117884 Hoxb6 Homeo box B6 −3.7462
mCG5070.2 9030425E11Rik RIKEN cDNA 9030425E11 gene −3.8063
mCG142672 −3.9345
mCG145172 Ces3 Carboxylesterase 3 −3.9714
mCG120048.1 −4.0282
mCG133246.1 9030624L02Rik RIKEN cDNA 9030624L02 gene −4.2692
mCG147785 Saa3 Serum amyloid A 3 −4.3242
mCG129928.1 Gsta2;Gsta1 Glutathione S-transferase, alpha 2 (Yc2);glutathione S-transferase, alpha −4.3994
1 (Ya)
mCG1178.1 Abcb1a ATP-binding cassette, subfamily B (MDR/TAP), member 1A −4.4645
mCG7690.2 Car12 Carbonic anyhydrase 12 −4.5391
mCG147297 −4.6285
mCG129934.1 −4.734
mCG142672 −4.755
mCG1544.3 Tmem25 Transmembrane protein 25 −5.1027
mCG125060 −5.2169
AK033690.1 −5.4956
mCG12622.1 Grp Gastrin releasing peptide −5.506
mCG18519.1 Fkbp5 FK506 binding protein 5 −6.2062
mCG23510.1 Ces2 Carboxylesterase 2 −6.3379
mCG23646.1 Gpt1 Glutamic pyruvic transaminase 1, soluble −6.7527
mCG20040.2 Car1 Carbonic anhydrase 1 −6.8954
mCG141930 Slc10a2 Solute carrier family 10, member 2 −7.1089
mCG142670 −7.7334
mCG144014 Es22 Esterase 22 −7.8314
mCG141694 −8.6598
mCG23407.1 9130231C15Rik RIKEN cDNA 9130231C15 gene −8.8614
mCG119535 Hsd3b2 Hydroxysteroid dehydrogenase-2, delta＜5＞-3-beta −10.737
mCG19918.2 Hsd3b6 Hydroxysteroid dehydrogenase-6, delta＜5＞-3-beta −13.204
mCG4201 Cyp2d26 Cytochrome P450, family 2, subfamily d, polypeptide 26 −16.967

Minus means that TNBS reduced gene expression.

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