Journal List > Korean J Cytopathol > v.19(2) > 1006529

Korean J Cytopathol. 2008 Sep;19(2):200-205. English.
Published online September 30, 2008.
Copyright © 2008 The Korean Society for Cytopathology
Imprint Cytology of Hibernoma -A Case Report-
Joon Hyuk Choi, M.D., and Duk Seop Shin, M.D.1
Department of Pathology, Yeungnam University College of Medicine, Daegu, Korea.
1Department of Orthopedic Surgery, Yeungnam University College of Medicine, Daegu, Korea.
Received July 17, 2008; Accepted September 04, 2008.


Hibernoma is a rare, benign adipose tumor composed of brown fat cells with eosinophilic granular or multivacuolated cytoplasm. The cytologic features of hibernoma have been rarely reported and may mimic other polygonal cell neoplasms. We report the imprint cytologic features of a case of hibernoma in the left thigh of a 68-year-old woman. Microscopic examination showed large, round, or polygonal brown fat cells. The cells were arranged in fragments or clusters. The nuclei were uniformly round with finely granular chromatin. The cytoplasm was multivacuolated or univacuolated. The abundant eosinophilic granular cytoplasm was also present. No nuclear atypia were present. Immunohistochemical staining showed that cells were positive for S-100 protein.

Keywords: Hibernoma; Cytology


Fig. 1
Magnetic resonance imaging. Coronal T1-weighted image shows a well defined mass with intermediate signal intensity (arrow) in the left thigh.
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Fig. 2
Positron emission tomography / computed tomography (PET/CT). PET/CT shows a well circumscribed, hypermetabolic soft tissue mass (arrow) in the left thigh.
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Fig. 3
Cytologic findings. (A) Tumor shows large, round, polygonal cells in a cellular fragment (Papanicolaou stain). (B) The background shows granular eosinophilic materials (H&E). (C) The tumor cells have multivacuolated cytoplasm. The nuclei of these cells are small and round and slightly eccentrically located in the cytoplasm (H&E). (D) Some tumor cells are round or polygonal and have eosinophilic granular cytoplasm (H&E). (E) Univacuolated fat cells are present (Papanicolaou stain). (F) These tumor cells are positive for S-100 protein (Immuohistochemical stain).
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Fig. 4
Gross finding. Tumor shows a well circumscribed, yellow-tan soft cut surface.
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Fig. 5
Histologic finding. The tumor is composed of large polygonal cells with multivacuolated or eosinophilic granular cytoplasm and matured adipocytes (H&E).
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Table 1
Published cases of hibernomas in the Korean literature
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