Journal List > Korean J Obstet Gynecol > v.53(7) > 1006441

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Byun, Jeong, Lee, Kim, Kim, Jeong, Sung, Lee, and Kim: Inhibition of cell growth and apoptosis in CaSki, cervical cancer cell line by arsenic compounds
Original Article
Korean Journal of Obstetrics and Gynecology

Korean Journal of Obstetrics and Gynecology 2010; 53(7): 616-625.

Published online: 31 July 2010

DOI: https://doi.org/10.5468/kjog.2010.53.7.616

Inhibition of cell growth and apoptosis in CaSki, cervical cancer cell line by arsenic compounds

Jung Mi Byun, M.D.1, Dae Hoon Jeong, M.D.1, 2, Dae Sim Lee, M.S.1, 2, Joo Ran Kim, Ph.D.1, 2, Young Nam Kim, M.D.1, 2, Eun Jeong Jeong, M.D.1, Moon Su Sung, M.D.1, Kyoung Bok Lee, M.D.1, Ki Tae Kim, M.D.1, 2

1Department of Obstetrics and Gynecology, Inje University College of Medicine, Busan Paik Hospital, Busan, Korea.

2Paik Institute for Clinical Research, Inje University College of Medicine, Busan Paik Hospital, Busan, Korea.

Corresponding author (obgynkkt@inje.ac.kr)

Received 1 April 2010       Accepted 1 June 2010

Copyright © 2010 Korean Society of Obstetrics and Gynecology

Abstract

Objective

To compare inhibition of cell growth and apoptosis in human cervical cancer cell lines (CaSki) by paclitaxel, cisplatin, arsenic trioxide and tetraarsenic oxide.

Methods

Inhibition of cell growth was determined by the water-soluble tetrazolium salts (WSTs) -1 assay. For apoptosis analysis in CaSki cell line treated with single or combination of two agents, CaSki cell line treated with each agent was stained with annexin-V/PI and flow cytometry was performed.

Results

Progression of apoptosis in CaSki cell line treated with paclitaxel, cisplatin, arsenic trioxide, and tetraarsenic oxide was time dependent. Inhibition of cell growth in CaSki cell line by paclitaxel, cisplatin, arsenic trioxide, and tetraarsenic oxide was dose and time dependent. Especially, tetraarsenic oxide was more effective in inhibition of CaSki cell growth compared to arsenic trioxide. Group treated with combination of cisplatin and tetraarsenic oxide showed more progressive apoptosis compared to other combination group.

Conclusion

Tetraarsenic oxide has more potent anti-tumor effects than other agents on CaSki cell line. We need to consider further study about antitumor effect of tetraarsenic oxide through clinical study.

Keywords: Cervical cancer, Paclitaxel, Cisplatin, Arsenic trioxide, Tetraarsenic oxide, Apoptosis

Figures and Tables

Fig. 1
Microscopic findings of CaSki cells treated with each single drug (paclitaxel, cisplatin, arsenic trioxide [As2O3], tetraarsenic oxide [As4O6]). Circles indicate normal CaSki cells. Arrows indicate apoptotic cells (×100).
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Fig. 2
Induction of apoptosis in CaSki cell line treated with paclitaxel, cisplatin, arsenic trioxide (As2O3), and tetraarsenic oxide (As4O6). CaSki cells were double stained with Annexin V and propium iodide (PI) followed by FACS analysis. The data are represented as % cell population. Induction of apoptosis in CaSki cell line treated with each agent was increased by time.
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Fig. 3
Induction of apoptosis in CaSki cell line treated with two agents (paclitaxel+cisplatin, paclitaxel+arsenic trioxide [As2O3], paclitaxel+tetraarsenic oxide [As4O6], cisplatin+arsenic trioxide [As2O3], cisplatin+tetraarsenic oxide [As4O6]). CaSki cells were double stained with Annexin V and propium iodide (PI) followed by FACS analysis. The data are represented as % cell population.
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Fig. 4
Apoptosis in CaSki cell line treated with two agents (paclitaxel+cisplatin, paclitaxel+arsenic trioxide [As2O3], paclitaxel+ tetraarsenic oxide [As4O6], cisplatin+arsenic trioxide [As2O3], cisplatin+tetraarsenic oxide [As4O6]). Apoptosis in CaSki cellline treated with cisplatin and tetraarsenic oxide (As4O6) was increased more significantly than with two agents.
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Fig. 5
(A) Apoptosis in CaSki cell line treated with single agent (paclitaxel, cisplatin, arsenic trioxide [As2O3], tetraarsenic oxide [As4O6]). The same experiment was repeated three times. The apoptosis was observed after 24 hours and 48 hours, (B) Apoptosis in CaSki cell line treated with two agents (paclitaxel+cisplatin, paclitaxel+arsenic trioxide [As2O3], paclitaxel+tetraarsenic oxide [As4O6], cisplatin+arsenic trioxide [As2O3], cisplatin+tetraarsenic oxide [As4O6]). Apoptosis in CaSki cellline treated with cisplatin and tetraarsenic oxide (As4O6) was increased more significantly than with two agents. *Paclitaxel, **Cisplatin.
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Table 1
Inhibition of cell growth in CaSki cell line by paclitaxel
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Cell viability showed as % control (p<0.05).

Table 2
Inhibition of cell growth in CaSki cell line by cisplatin
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Cell viability showed as % control (p<0.05).

Table 3
Inhibition of cell growth in CaSki cell line by arsenic trioxide (As2O3)
kjog-53-616-i003

Cell viability showed as % control (p<0.05).

Table 4
Inhibition of cell growth in CaSki cellline by tetraarsenic oxide (As4O6)
kjog-53-616-i004

Cell viability showed as % control (p<0.05).

Table 5
The IC50 concentration of paclitaxel, cisplatin, arsenic trioxide, and tetraarsenic oxide in CaSki cell line.
kjog-53-616-i005

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