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You, Kim, Lee, Park, Kim, and Chun: Association of preterm birth with IFN-γ, IL-10, and CYP 1A1 gene in Korean women
Original Article
Korean Journal of Obstetrics and Gynecology

Korean Journal of Obstetrics and Gynecology 2010; 53(7): 608-615.

Published online: 31 July 2010

DOI: https://doi.org/10.5468/kjog.2010.53.7.608

Association of preterm birth with IFN-γ, IL-10, and CYP 1A1 gene in Korean women

Si Yeon You, M.D., Jung Myung Kim, M.D., Kyung A Lee, M.D., Ph.D., Mi Hye Park, M.D., Ph.D., Young Ju Kim, M.D., Ph.D., Sun Hee Chun, M.D., Ph.D.

Department of Obstetrics and Gynecology, Ewha Womans University School of Medicine, Seoul, Korea.

Corresponding author (kkyj@ewha.ac.kr)

Received 30 April 2010       Accepted 4 June 2010

Copyright © 2010 Korean Society of Obstetrics and Gynecology

Abstract

Objective

To investigate the association between preterm birth and cytokine genes (IFN-γ, interleukin (IL)-10) in intrauterine infection and enzyme gene (CYP1A1) in oxidative stress response.

Methods

This study involved a case-control study conducted at Ewha Womans University Hospital in Seoul, Korea. Subjects with preterm deliveries (<37 weeks of gestation) and normal controls with term deliveries (≥37 weeks of gestation) were selected from gravidas who had undergone prenatal examinations in the hospital and were followed until infant delivery. The weight, height, and blood samples of each participants were obtained according to standard protocols. We included subjects who gave birth to a singleton infant and had a gestational age between 24 and 42 weeks. Mutiple births, stillbirths, and congenital anomalies were excluded. Finally, 164 gravidas with preterm births and 305 normal controls with term deliveries were enrolled in the present study.

Results

Preterm delivery group and term delivery group had significant difference in gestational age and neonatal body weight (P<0.0001). There were no statistically significant association between preterm birth and IFN-γ, IL-10, CYP1A1 genes (P>0.05).

Conclusion

In this study, IFN-γ (874A/T), IL-10 (1082A/G), IL-10 (819C/T), IL-10 (592A/C) and CYP1A1 (T6235C), CYP1A1 (Ile462val [A/G]) genes had no significant association with preterm birth.

Keywords: Preterm birth, IFN-γ, IL-10, CYP1A1 gene

Figures and Tables

Table 1
Primer sequence of gene
kjog-53-608-i001

SNP: single nucleotide polymorphism, RS: reference SNP, CYP1A1: Cytochrome P450 1A1, IL: interleukin, IFNG: interferone-γ.

Table 2
Clinical characteristics of the patients
kjog-53-608-i002

NS: not significant.

Table 3
The allele and genotype frequencies of the SNPs in the IFN-γ gene and risk of preterm delivery
kjog-53-608-i003

SNP: single nucleotide polymorphism, OR: odds ratio, IFNG: interferone-γ, NS: not significant, REF: reference.

*Adjusted for maternal age, body mass index at delivery, gestational diabetes mellitus, preeclampsia, sex of fetus, birth order.

Table 4
The allele and genotype frequencies of the SNPs in the IL-10 gene and risk of preterm delivery
kjog-53-608-i004

SNP: single nucleotide polymorphism, OR: odds ratio, IL: interleukin, NS: not significant, REF: reference.

* Adjusted for maternal age, body mass index at delivery, gestational diabetes mellitus, preeclampsia, sex of fetus, birth order.

Table 5
The allele and genotype frequencies of the SNPs in the CYP1A1 gene and risk of preterm delivery
kjog-53-608-i005

SNP: single nucleotide polymorphism, OR: odds ratio, NS: not significant, REF: reference.

* Adjusted for maternal age, body mass index at delivery, gestational diabetes mellitus, preeclampsia, sex of fetus, birth order.

Table 6
Haplotyping of IL-10 gene and risk of preterm delivery
kjog-53-608-i006

SNP: single nucleotide polymorphism, OR: odds ratio, NS: not significant, REF: reference.

*Adjusted for maternal age, body mass index at delivery, gestational diabetes mellitus, preeclampsia, sex of fetus, birth order.

Table 7
Haplotyping of CYP1A1 gene and risk of preterm delivery
kjog-53-608-i007

SNP: single nucleotide polymorphism, OR: odds ratio, NS: not significant, REF: reference.

*Adjusted for maternal age, body mass index at delivery, gestational diabetes mellitus, preeclampsia, sex of fetus, birth order.

References

1. Goldenberg RL, Hauth JC, Andrews WW. Intrauterine infection and preterm delivery. N Engl J Med. 2000. 342:1500–1507.
2. Speer EM, Gentile DA, Zeevi A, Pillage G, Huo D, Skoner DP. Role of single nucleotide polymorphisms of cytokine genes in spontaneous preterm delivery. Hum Immunol. 2006. 67:915–923.
3. Suh YJ, Kim YJ, Park H, Park EA, Ha EH. Oxidative stress-related gene interactions with preterm delivery in Korean women. Am J Obstet Gynecol. 2008. 198:541e1–e7.
4. Greig PC, Herbert WN, Robinette BL, Teot LA. Amniotic fluid interleukin-10 concentrations increase through pregnancy and are elevated in patients with preterm labor associated with intrauterine infection. Am J Obstet Gynecol. 1995. 173:1223–1227.
5. Turner DM, Williams DM, Sankaran D, Lazarus M, Sinnott PJ, Hutchinson IV. An investigation of polymorphism in the interleukin-10 gene promoter. Eur J Immunogenet. 1997. 24:1–8.
6. Wang X, Zuckerman B, Pearson C, Kaufman G, Chen C, Wang G, et al. Maternal cigarette smoking, metabolic gene polymorphism, and infant birth weight. JAMA. 2002. 287:195–202.
7. Hillier SL, Witkin SS, Krohn MA, Watts DH, Kiviat NB, Eschenbach DA. The relationship of amniotic fluid cytokines and preterm delivery, amniotic fluid infection, histologic chorioamnionitis, and chorioamnion infection. Obstet Gynecol. 1993. 81:941–948.
8. Romero R, Mazor M, Sepulveda W, Avila C, Copeland D, Williams J. Tumor necrosis factor in preterm and term labor. Am J Obstet Gynecol. 1992. 166:1576–1587.
9. Romero R, Gomez R, Ghezzi F, Yoon BH, Mazor M, Edwin SS, et al. A fetal systemic inflammatory response is followed by the spontaneous onset of preterm parturition. Am J Obstet Gynecol. 1998. 179:186–193.
10. Yamada H, Sata F, Kato EH, Saijo Y, Kataoka S, Morikawa M, et al. A polymorphism in the CYP17 gene and intrauterine fetal growth restriction. Mol Hum Reprod. 2004. 10:49–53.
11. Curry AE, Vogel I, Drews C, Schendel D, Skogstrand K, Flanders WD, et al. Mid-pregnancy maternal plasma levels of interleukin 2, 6, and 12, tumor necrosis factor-alpha, interferon-gamma, and granulocyte-macrophage colony-stimulating factor and spontaneous preterm delivery. Acta Obstet Gynecol Scand. 2007. 86:1103–1110.
12. Andrews WW, Hauth JC, Goldenberg RL, Gomez R, Romero R, Cassell GH. Amniotic fluid interleukin-6: correlation with upper genital tract microbial colonization and gestational age in women delivered after spontaneous labor versus indicated delivery. Am J Obstet Gynecol. 1995. 173:606–612.
13. Wegmann TG, Lin H, Guilbert L, Mosmann TR. Bidirectional cytokine interactions in the maternal-fetal relationship: is successful pregnancy a TH2 phenomenon? Immunol Today. 1993. 14:353–356.
14. Daher S, Shulzhenko N, Morgun A, Mattar R, Rampim GF, Camano L, et al. Associations between cytokine gene polymorphisms and recurrent pregnancy loss. J Reprod Immunol. 2003. 58:69–77.
15. Moura E, Mattar R, de Souza E, Torloni MR, Goncalves-Primo A, Daher S. Inflammatory cytokine gene polymorphisms and spontaneous preterm birth. J Reprod Immunol. 2009. 80:115–121.
16. Hill JA. T-helper 1-type immunity to trophoblast: evidence for a new immunological mechanism for recurrent abortion in women. Hum Reprod. 1995. 10:Suppl 2. 114–120.
17. Jenkins C, Roberts J, Wilson R, MacLean MA, Shilito J, Walker JJ. Evidence of a T(H) 1 type response associated with recurrent miscarriage. Fertil Steril. 2000. 73:1206–1208.
18. Marzi M, Vigano A, Trabattoni D, Villa ML, Salvaggio A, Clerici E, et al. Characterization of type 1 and type 2 cytokine production profile in physiologic and pathologic human pregnancy. Clin Exp Immunol. 1996. 106:127–133.
19. Scholl TO, Stein TP. Oxidant damage to DNA and pregnancy outcome. J Matern Fetal Med. 2001. 10:182–185.
20. Hirvonen A. Genetic factors in individual responses to environmental exposures. J Occup Environ Med. 1995. 37:37–43.
21. Roberts AK, Monzon-Bordonaba F, Van Deerlin PG, Holder J, Macones GA, Morgan MA, et al. Association of polymorphism within the promoter of the tumor necrosis factor alpha gene with increased risk of preterm premature rupture of the fetal membranes. Am J Obstet Gynecol. 1999. 180:1297–1302.
22. Genc MR, Gerber S, Nesin M, Witkin SS. Polymorphism in the interleukin-1 gene complex and spontaneous preterm delivery. Am J Obstet Gynecol. 2002. 187:157–163.
23. Kazzi SN, Jacques SM, Qureshi F, Quasney MW, Kim UO, Buhimschi IA. Tumor necrosis factor-alpha allele lymphotoxin-alpha+250 is associated with the presence and severity of placental inflammation among preterm births. Pediatr Res. 2004. 56:94–98.
24. Veith GL, Rice GE. Interferon gamma expression during human pregnancy and in association with labour. Gynecol Obstet Invest. 1999. 48:163–167.
25. Fortunato SJ, Menon R, Lombardi SJ. Interleukin-10 and transforming growth factor-beta inhibit amniochorion tumor necrosis factor-alpha production by contrasting mechanisms of action: therapeutic implications in prematurity. Am J Obstet Gynecol. 1997. 177:803–809.
26. Hassan MI, Aschner Y, Manning CH, Xu J, Aschner JL. Racial differences in selected cytokine allelic and genotypic frequencies among healthy, pregnant women in North Carolina. Cytokine. 2003. 21:10–16.
27. Toescu V, Nuttall SL, Martin U, Kendall MJ, Dunne F. Oxidative stress and normal pregnancy. Clin Endocrinol (Oxf). 2002. 57:609–613.
28. Maulik D, Qayyum I, Powell SR, Karantza M, Mishra OP, Delivoria-Papadopoulos M. Post-hypoxic magnesium decreases nuclear oxidative damage in the fetal guineapig brain. Brain Res. 2001. 890:130–136.
29. Li Y, Wang H. In utero exposure to tobacco and alcohol modifies neurobehavioral development in mice offspring: consideration a role of oxidative stress. Pharmacol Res. 2004. 49:467–473.
30. Chen D, Hu Y, Yang F, Li Z, Wu B, Fang Z, et al. Cytochrome P450 gene polymorphisms and risk of low birth weight. Genet Epidemiol. 2005. 28:368–375.
31. Park CH, Shin JC, Yang DE, Park IY, Ko HS, Kim YH, et al. Association of the polymorphisms in CYP1A1 and CYP1A2 genes with preterm premature rupture of membranes. Korean J Obstet Gynecol. 2004. 47:2319–2324.
32. Goldenberg RL, Culhane JF, Iams JD, Romero R. Epidemiology and causes of preterm birth. Lancet. 2008. 371:75–84.
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