Journal List > Korean J Obstet Gynecol > v.53(7) > 1006440

You, Kim, Lee, Park, Kim, and Chun: Association of preterm birth with IFN-γ, IL-10, and CYP 1A1 gene in Korean women

Abstract

Objective

To investigate the association between preterm birth and cytokine genes (IFN-γ, interleukin (IL)-10) in intrauterine infection and enzyme gene (CYP1A1) in oxidative stress response.

Methods

This study involved a case-control study conducted at Ewha Womans University Hospital in Seoul, Korea. Subjects with preterm deliveries (<37 weeks of gestation) and normal controls with term deliveries (≥37 weeks of gestation) were selected from gravidas who had undergone prenatal examinations in the hospital and were followed until infant delivery. The weight, height, and blood samples of each participants were obtained according to standard protocols. We included subjects who gave birth to a singleton infant and had a gestational age between 24 and 42 weeks. Mutiple births, stillbirths, and congenital anomalies were excluded. Finally, 164 gravidas with preterm births and 305 normal controls with term deliveries were enrolled in the present study.

Results

Preterm delivery group and term delivery group had significant difference in gestational age and neonatal body weight (P<0.0001). There were no statistically significant association between preterm birth and IFN-γ, IL-10, CYP1A1 genes (P>0.05).

Conclusion

In this study, IFN-γ (874A/T), IL-10 (1082A/G), IL-10 (819C/T), IL-10 (592A/C) and CYP1A1 (T6235C), CYP1A1 (Ile462val [A/G]) genes had no significant association with preterm birth.

Figures and Tables

Table 1
Primer sequence of gene
kjog-53-608-i001

SNP: single nucleotide polymorphism, RS: reference SNP, CYP1A1: Cytochrome P450 1A1, IL: interleukin, IFNG: interferone-γ.

Table 2
Clinical characteristics of the patients
kjog-53-608-i002

NS: not significant.

Table 3
The allele and genotype frequencies of the SNPs in the IFN-γ gene and risk of preterm delivery
kjog-53-608-i003

SNP: single nucleotide polymorphism, OR: odds ratio, IFNG: interferone-γ, NS: not significant, REF: reference.

*Adjusted for maternal age, body mass index at delivery, gestational diabetes mellitus, preeclampsia, sex of fetus, birth order.

Table 4
The allele and genotype frequencies of the SNPs in the IL-10 gene and risk of preterm delivery
kjog-53-608-i004

SNP: single nucleotide polymorphism, OR: odds ratio, IL: interleukin, NS: not significant, REF: reference.

* Adjusted for maternal age, body mass index at delivery, gestational diabetes mellitus, preeclampsia, sex of fetus, birth order.

Table 5
The allele and genotype frequencies of the SNPs in the CYP1A1 gene and risk of preterm delivery
kjog-53-608-i005

SNP: single nucleotide polymorphism, OR: odds ratio, NS: not significant, REF: reference.

* Adjusted for maternal age, body mass index at delivery, gestational diabetes mellitus, preeclampsia, sex of fetus, birth order.

Table 6
Haplotyping of IL-10 gene and risk of preterm delivery
kjog-53-608-i006

SNP: single nucleotide polymorphism, OR: odds ratio, NS: not significant, REF: reference.

*Adjusted for maternal age, body mass index at delivery, gestational diabetes mellitus, preeclampsia, sex of fetus, birth order.

Table 7
Haplotyping of CYP1A1 gene and risk of preterm delivery
kjog-53-608-i007

SNP: single nucleotide polymorphism, OR: odds ratio, NS: not significant, REF: reference.

*Adjusted for maternal age, body mass index at delivery, gestational diabetes mellitus, preeclampsia, sex of fetus, birth order.

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