Journal List > Korean J Obstet Gynecol > v.53(12) > 1006373

Korean J Obstet Gynecol. 2010 Dec;53(12):1100-1109. Korean.
Published online December 21, 2010.  https://doi.org/10.5468/kjog.2010.53.12.1100
Copyright © 2010 Korean Society of Obstetrics and Gynecology
The study of association between human papillomavirus and Chlamydia trachomatis infection in cervical intraepithelial neoplasia
Jung-Sun Yang, M.D., Yun-Hee Jeong, M.D. and Jong-Soo Kim, M.D.
Department of Obstetrics and Gynecology, Dankook University College of Medicine, Cheonan, Korea.

Corresponding author (Email: soo8541@hanmail.net )
Received July 21, 2010; Revised October 18, 2010; Accepted November 17, 2010.

Abstract

Objective

The aim of this study is to comparing the prevalence and correlationships between human papillomavirus (HPV) and Chlamydia trachomatis (CT) infection in cervical samples among women with abnormal cervical cytology.

Methods

This study was included three hundred seventy four patients with a abnormal liquid-based cytology in Dankook University hospital. All of them underwent HPV DNA test and CT analysis with polymerase chain reaction. All patients also went through colposcopic directed cervical biopsies or Loop Electrosurgical Excision Procedure, conization. The histo-pathologic results were classified as normal, cervical intraepithelial neoplasia (CIN) 1, CIN 2, CIN 3 and carcinoma in situ (CIS).

Results

Abnormal liquid-based cytology patients were pathologically proven to have CIN. Among 374 patients, the number of within normal limits (chronic cervicitis) and koilocytosis was 186 cases (49.7%), CIN 1, 64 cases (17.1%), CIN 2 16 cases (4.3%) CIN 3, 55 cases (14.7%), and CIS, 53 cases (14.2%). HPV DNA positive patients were 235 cases and HPV DNA negative patients were 139 cases. The impact of CT infection seems not to interfere with the development or even the progression of CIN. Thirty one patients had positive infection of CT (8.3%) and 343 patients were negative infection of CT (91.7%). Both HPV and CT positive infected patients were 25 cases (6.7%) in abnormal cytologic women. The correlation between HPV and CT DNA positive among women with abnormal cytology was statistically significant. (P=0.022)

Conclusion

This study suggests that CT infection is associated with HPV infection, but the clinical significance of the association between CT and HPV infection remains to be elucidated.

Keywords: Cervical intraepithelial neoplasia; Human papilloma virus; Chlamydia trachomatis; Liquid-based cytology

Figures


 Fig. 1
Species-specific DNA amplification by multiples polymerase chain reaction in clinical specimens. M: STD6 marker: Trichomonas vaginalis (580 bp), Mycoplasma hominis (502 bp), Ureaplasma urealyticum (435 bp), Chlamydia trachomatis (348 bp), Mycoplasma genitalium (253 bp), Neisseria gonorrhoeae (214 bp). N: negative. 1~6: clinical samples.

Sample 1 Trichomonas vaginalis, Chlamydia trachomatis.

Sample 2 Ureaplasma urealyticum.

Sample 3 Mycoplasma hominis, Chlamydia trachomatis.

Sample 4 Neisseria gonorrhoeae.

Sample 5 Mycoplasma genitalium.

Sample 6 Ureaplasma urealyticum, Chlamydia trachomatis, Mycoplasma genitalium, Neisseria gonorrhoeae.
Click for larger image

Tables


 Table 1
Histologic diagnosis of each abnormal liquid-based cytology result
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Table 2
Correlation between cytopathological results and type specific HPV DNA in abnormal cervical cytologic women
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Table 3
Correlation between cytopathological results and C. trachomatis DNA detection
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Table 4
Correlation between cytopathological results and HPV and C. trachomatis co-infection
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Table 5
The correlation between HPV and C. trachomatis DNA positive among women with abnormal cytology
Click for larger image

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