Journal List > Korean J Urol > v.48(5) > 1004913

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Jung, Han, Yun, Kim, Lee, Lee, and Kim: Analysis of the NF-κB and Apoptosis Inducing Genes in Bladder Tumor
Original Article
Korean Journal of Urology

Korean Journal of Urology 2007; 48(5): 483-488.

Published online: 31 May 2007

DOI: https://doi.org/10.4111/kju.2007.48.5.483

Analysis of the NF-κB and Apoptosis Inducing Genes in Bladder Tumor

Pildu Jung, Kwang-Hee Han, Seok Joong Yun, Yong June Kim, Sang-Cheol Lee, Hyung-Lae Lee1, Wun-Jae Kim

Department of Urology, Chungbuk National University College of Medicine, Cheongju, Korea.

1Department of Urology, School of Medicine, Kyung Hee University, Seoul, Korea.

Corresponding author (wjkim@chungbuk.ac.kr)

Received 9 February 2007       Accepted 18 April 2007

Copyright © 2007 The Korean Urological Association

Abstract

Purpose

A multi-subunit transcription factor NF-κB mediates the antiapoptotic signals in several cancer cell lines and it is activated in a broad range of human tumors. In this study, we investigated whether the expression levels of the NF-κB and the apoptosis inducing genes were related to the pathogenesis and clinical properties of human bladder tumor.

Materials and Methods

The expressions of NF-κB, BCL2-associated X protein (BAX), BCL2-associated death protein (BAD) and BH3-interacting domain death agonist protein (BID) were investigated by performing immunohistochemical staining on 133 archival bladder tissue paraffin blocks; these blocks included 122 transitional cell carcinomas of the urinary bladder and 11 normal bladder mucosae.

Results

The expression levels of NF-κB were significantly higher in the bladder tumors than those of the normal bladder mucosae (p=0.001). The expression levels of BAX in the superficial and low-grade (grade 1 and 2) bladder tumors were significantly enhanced more than those of the high-grade and invasive cases (p=0.042 and p=0.045, respectively), while the expression levels of BAD in the tumor tissues and low-grade tumors were significantly elevated compared with those of the normal mucosae and high grade tumor (p=0.007 and p=0.048, respectively). But the expressions of BID were not correlated with any pathologic and clinical properties.

Conclusions

The expressions of the NF-κB and apoptosis inducing genes such as BAX and BAD are strongly associated with the pathogenesis and clinical properties of bladder tumor.

Keywords: NF-κB, BCL2-associated X protein, BCL2-associated death protein, BH3 interacting domain death agonist protein, Bladder tumor

Figures and Tables

Fig. 1
The immunohistochemical staining. NF-κB in the (A) normal mucosa, (B) superficial tumor and (C) invasive tumor of bladder. BAX in (D) normal mucosa, (E) superficial tumor, (F) invasive tumor of bladder. BAD in the (G) normal mucosa, (H) superficial tumor and (I) invasive tumor. BID in (J) normal mucosa, (K) superficial tumor and (L) invasive tumor of bladder. NF-κB: nuclear factor of kappa B, BAX: BCL2- associated X protein, BAD: BCL2-associated death protein, BID: BH3- interacting domain death agonist protein.
kju-48-483-g001
Table 1
Demographic data of the 122 patients with primary bladder transitional cell carcinoma
kju-48-483-i001

*: year±standard deviation

Table 2
Protein expression levels of NF-κB, BAX, BAD and BID in bladder tumors
kju-48-483-i002

NF-κB: nuclear factor of kappa B, BAX: BCL2-associated X protein, BAD: BCL2-associated death protein, BID: BH3-interacting domain death agonist protein

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