Abstract
Purpose
This study was performed to evaluate the relationship of the expressions of clusterin with Ki-67 and the clinicopathological factors and significance of clusterin expression in human renal cell carcinoma (RCC).
Materials and Methods
Normal kidney (n=26) and RCC tissues (n=111) were obtained from 111 patients who had undergone a radical or partial nephrectomy. The expressions of clusterin and Ki-67 protein were analysed using immunohistochemical staining. The medical records of the patients were retrospectively reviewed to evaluate the clinicopathological factors.
Results
In contrast to the clusterin and Ki-67 expressions of 30.8 and 11.5%, respectively in the normal kidney (n=26), those in the RCC tissues were 93.7 and 28.8% (n=111), respectively (p<0.05). In contrast to the normal tissues, Ki-67 staining was significantly correlated with the expression of clusterin in the RCC tissues (p<0.05). The expression level of clusterin protein in the RCC tissues was significantly related to the tumor stage and grade (p<0.05), but not to age, gender or histological cell type (p>0.05). Furthermore, the recurrence-free survival in patients with strong clusterin expression was significantly lower than in those with weak expression (p<0.05).
Figures and Tables
Fig. 1
Immunohistochemical staining of clusterin. (A) Negative, (B) weak positive, (C) strong positive (arrow: positive stain of clusterin, ×200).
![kju-48-244-g001](/upload/SynapseData/ArticleImage/1020kju/kju-48-244-g001.jpg)
Fig. 2
Immunohistochemical staining of Ki-67. (A) Negative, (B) weak positive, (C) strong positive (arrow: positive stain of Ki-67, ×200).
![kju-48-244-g002](/upload/SynapseData/ArticleImage/1020kju/kju-48-244-g002.jpg)
Fig. 3
Recurrence-free survival of patients with a renal cell carcinoma according to the clusterin expression status (p=0.043).
![kju-48-244-g003](/upload/SynapseData/ArticleImage/1020kju/kju-48-244-g003.jpg)
Fig. 4
Recurrence-free survival of patients with a renal cell carcinoma according to the Ki-67 expression status (p=0.047).
![kju-48-244-g004](/upload/SynapseData/ArticleImage/1020kju/kju-48-244-g004.jpg)
Fig. 5
Recurrence-free survival of patients with a renal cell carcinoma according to both the clusterin and Ki-67 expression stati (p=0.024).
![kju-48-244-g005](/upload/SynapseData/ArticleImage/1020kju/kju-48-244-g005.jpg)
Table 1
Results of clusterin immunohistochemical staining in normal kidney and renal cell carcinoma tissues
![kju-48-244-i001](/upload/SynapseData/ArticleImage/1020kju/kju-48-244-i001.jpg)
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