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Park, Kim, Lee, Lee, Lee, Lee, and Chung: The Relationship of Clusterin Expression with Ki-67 Expression and Clinicopathological Factors in Human Renal Cell Carcinoma
Original Article
Korean Journal of Urology

Korean Journal of Urology 2007; 48(3): 244-251.

Published online: 31 March 2007

DOI: https://doi.org/10.4111/kju.2007.48.3.244

The Relationship of Clusterin Expression with Ki-67 Expression and Clinicopathological Factors in Human Renal Cell Carcinoma

Hyun Cheol Park, Jeong Man Kim, Chang Leol Lee, Wan Lee, Sang Don Lee, Jeong Zoo Lee, Moon Kee Chung

Department of Urology, College of Medicine, Pusan National University, Busan, Korea.

Corresponding author (mkchung@pusan.ac.kr)

Received 27 July 2006       Accepted 27 January 2007

Copyright © 2007 The Korean Urological Association

Abstract

Purpose

This study was performed to evaluate the relationship of the expressions of clusterin with Ki-67 and the clinicopathological factors and significance of clusterin expression in human renal cell carcinoma (RCC).

Materials and Methods

Normal kidney (n=26) and RCC tissues (n=111) were obtained from 111 patients who had undergone a radical or partial nephrectomy. The expressions of clusterin and Ki-67 protein were analysed using immunohistochemical staining. The medical records of the patients were retrospectively reviewed to evaluate the clinicopathological factors.

Results

In contrast to the clusterin and Ki-67 expressions of 30.8 and 11.5%, respectively in the normal kidney (n=26), those in the RCC tissues were 93.7 and 28.8% (n=111), respectively (p<0.05). In contrast to the normal tissues, Ki-67 staining was significantly correlated with the expression of clusterin in the RCC tissues (p<0.05). The expression level of clusterin protein in the RCC tissues was significantly related to the tumor stage and grade (p<0.05), but not to age, gender or histological cell type (p>0.05). Furthermore, the recurrence-free survival in patients with strong clusterin expression was significantly lower than in those with weak expression (p<0.05).

Conclusions

These findings suggest that clusterin may be involved in the progression of RCC and; therefore, a useful prognostic variable in patients with an RCC.

Keywords: Renal cell carcinoma, Clusterin, Ki-67 antigen, Clinicopathological factor

Figures and Tables

Fig. 1
Immunohistochemical staining of clusterin. (A) Negative, (B) weak positive, (C) strong positive (arrow: positive stain of clusterin, ×200).
kju-48-244-g001
Fig. 2
Immunohistochemical staining of Ki-67. (A) Negative, (B) weak positive, (C) strong positive (arrow: positive stain of Ki-67, ×200).
kju-48-244-g002
Fig. 3
Recurrence-free survival of patients with a renal cell carcinoma according to the clusterin expression status (p=0.043).
kju-48-244-g003
Fig. 4
Recurrence-free survival of patients with a renal cell carcinoma according to the Ki-67 expression status (p=0.047).
kju-48-244-g004
Fig. 5
Recurrence-free survival of patients with a renal cell carcinoma according to both the clusterin and Ki-67 expression stati (p=0.024).
kju-48-244-g005
Table 1
Results of clusterin immunohistochemical staining in normal kidney and renal cell carcinoma tissues
kju-48-244-i001

RCC: renal cell carcinoma. *: chi-square test

Table 2
Results of Ki-67 immunohistochemical staining in normal kidney and renal cell carcinoma tissues
kju-48-244-i002

RCC: renal cell carcinoma. *: chi-square test

Table 3
The correlation of clusterin expression in renal cell carcinoma tissues, with the clinicopathological factors and cell proliferative activity
kju-48-244-i003

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