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Lee, Lee, Cho, Cho, Jang, and Lee: DNA Profiling via Short Tandem Repeat Analysis by Using Serum Samples
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Korean J Leg Med 2013;37(4):220-223.

Published online: 17 January 2013

DOI: https://doi.org/10.7580/kjlm.2013.37.4.220

DNA Profiling via Short Tandem Repeat Analysis by Using Serum Samples

Ji Hyun Lee1, Hye Young Lee1, Sohee Cho1, Joo Youn Cho3, In Jin Jang3, Soong Deok Lee1, 2

1Department of Forensic Medicine, College of Medicine, Seoul, Korea

2Institute of Forensic Science, Seoul National University, College of Medicine, Seoul, Korea

3Department of Pharmacology, College of Medicine, Seoul National University, Seoul, Korea

이 논문은 약물유전체센터:약물유해반응 연구센터(800-20130245)와 2012년도 한 국연구재단 바이오∙의료기술개발사업 (No. 2012-0009833)의 지원을 받아 수행 되었습니다. 책임저자 : 이숭덕 (110-799) 서울시 종로구 연건동 대학로 103번지, 서울대학교 의과대학 법의학교 실 전화 : +82-2-740-8359 FAX : +82-2-764-8340 E-mail : sdlee@snu.ac.kr

Received 16 September 2013       Revised 14 October 2013       Accepted 25 November 2013

Copyright © 2013 The Korean Society for Legal Medicine

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Serum is free of cellular components. Because DNA is located in the nuclei or mitochondria of cells, serum could be assumed DNA free. Few previously published case reports to date have used serum for DNA typing. Here, we report on human genotyping via short tandem repeat (STR) analysis using serum as a sample, and discuss problems involved in the process.

Keywords: Key Words, Serum, DNA Extraction, STR

REFERENCES

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Fig. 1.
The former electropherogram (a, c, e) obtained from blood, and the latter (b, d, f) obtained from serum. (b) indicates allele drop-out of the serum's 11 allele at locus TPOX and 10 allele at locus D5S818. (d) indicates allele drop-in of the serum's 28 allele at locus D2S1338 and allele drop-out of the serum's 15 allele at locus D18S51. (f) indicates drop-in of the serum's 26 allele and drop-out of the 21, 23 allele at locus D22S1045. Also indicates unamplified at locus D19S433.
kjlm-37-220f1.tif
Table 1.
The Number of Amplified Allele and the Percentage of Drop-out, Drop-in Ratio
  Mean concentration (ng/㎕) The number of peaks Allele drop-out Allele drop-in
serum-1 5.65 48, 48 0/48, 0/48 (0%) 0/48, 0/48 (0%)
serum-2 3.9 48, 48 7/48, 3/48 (14.6%, 6.3%) 2/48, 3/48 (4.2%, 6.3%)
serum-3 4.2 46, 48 13/46, 8/48 (28.3%, 16.7%) 12/46 4/48 (26.1%, 8.3%)
serum-4 3.85 48, 48 1/48, 0/48 (2.1%, 0%) 1/48, 0/48 (2.1%, 0%)
serum-5 3.8 46, 48 1/46, 1/48 (2.2%, 2.1%) 2/46, 2/48 (4.3%, 4.2%)
serum-6 4.15 48, 48 0/48, 0/48 (0%) 1/48, 0/48 (2.1%, 0%)
serum-7 3.5 44, 48 4/44, 2/48 (9.1%, 4.2%) 6/44, 3/48 (13.6%, 6.3%)
serum-8 8.1 48, 48 4/48, 0/48 (8.3%, 0%) 3/48, 0/48 (6.3%, 0%)
serum-9 2.2 48, 48 3/48, 0/48 (6.3%, 0%) 0/48, 0/48 (0%)
serum-10 3.15 48, 48 3/48, 3/48 (6.3%, 6.3%) 6/48, 2/48 (12.5%, 4.2%)
serum-11 3.9 36, 42 13/36, 11/42 (36.1%, 26.2%) 8/36, 7/42 (22.2%, 16.7%)
serum-12 2.1 42, 48 9/42, 7/48 (21.4%, 14.6%) 6/42, 6/48 (14.3%, 12.5%)
serum-13 3.9 40, 48 13/40, 18/48 (32.5%, 37.5%) 11/40, 20/48 (27.5%, 41.7%)

The percentage of drop-out, drop-in peaks was determined by dividing the number of peaks by the total number of real peaks. This table is represents the results of repeated experiments.

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