Increased Interleukin-17 Expression in Patients with Idiopathic Inflammatory Myopathies

Seung-Hoon Baek; Jun-Hee Lee; Geun-Tae Kim; Joung-Wook Lee; Mi-Ra Cho; Ju-In Kim; Sun-Hee Lee; Dae-Seong Kim; Sung-Il Kim

doi:10.4078/jkra.2008.15.1.118

Journal List > J Korean Rheum Assoc > v.15(2) > 1003612

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Baek, Lee, Kim, Lee, Cho, Kim, Lee, Kim, and Kim: Increased Interleukin-17 Expression in Patients with Idiopathic Inflammatory Myopathies

Original Article

J Korean Rheum Assoc 2008;15(2):118-122.

Published online: 20 March 2008

DOI: https://doi.org/10.4078/jkra.2008.15.1.118

Increased Interleukin-17 Expression in Patients with Idiopathic Inflammatory Myopathies

Seung-Hoon Baek^∗∗∗, Jun-Hee Lee, Geun-Tae Kim, Joung-Wook Lee, Mi-Ra Cho, Ju-In Kim^∗∗, Sun-Hee Lee, Dae-Seong Kim^∗, Sung-Il Kim

Department of Internal Medicine, College of Medicine, Pusan National University, Busan, Korea

^∗Department of Neurology, College of Medicine, Pusan National University, Busan, Korea

^∗∗Department of Research Institute, College of Medicine, Pusan National University, Busan, Korea

^∗∗∗Department of Internal Medicine, Busan St. Mary's Medical Center, Busan, Korea

Received 3 January 2008 Revised 27 March 2008

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective

Idiopathic inflammatory myopathies (IIMs) are systemic autoimmune diseases characterized by infiltration of T lymphocytes, monocytes, and macrophages in muscle tissues. Interleukin-17 (IL-17), a Th17 cytokine, has potent pro-inflammatory actions and plays a role in autoimmune diseases. We investigated the expression of IL-17 in muscle tissues of patients with IIMs.

Methods

We measured the IL-17 mRNA level of muscle tissues from 14 patients with IIMs (9 patients with dermatomyositis and 5 patients with polymyositis) by real-time RT-PCR and compared with controls. We also performed an immunohistochemical stain to detect IL-17 expression.

Results

The expressions of IL-17 were significantly enhanced in IIMs than controls. In immunohistochemistry, IL-17 was expressed in perimysial, endomysial and perivascular infiltrating inflammatory cells.

Conclusion

These results suggest that IL-17 plays a role in the immunopathogenesis of IIMs.

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Keywords: Interleukin-17, Idiopathic inflammatory myopathies, Dermatomyositis, Polymyositis

References

1. Briani C, Doria A, Sarzi-Puttini P, Dalakas MC. Update on idiopathic inflammatory myopathies. Autoimmunity. 2006; 39:161–70.

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9. Bohan A, Peter JB. Polymyositis and dermatomyositis (first of two parts). N Engl J Med. 1975; 292:403–7.

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Fig. 1.

Immunohistochemistry of IL-17 in muscle tissues from patients with IIMs (B, C) and control (A). In DM (B) and PM (C), IL-17 was expressed in perimysial, endomysialand perivascular infiltrating inflammatory cells (arrow). Muscle tissue from control was negative (A). Magnification, ×200. IIMs: idiopathic inflammatory myopathies, DM: dermatomyositis, PM: polymyositis.

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Table 1.

Demographic, clinical, and laboratory characteristics in 14 patients with idiopathic inflammatory myopathies

Patient/Sex	Age	Diagnosis	Disease duration (months)	CK, units/L	Anti-Jo-1 Antibody	Relative levels of IL-17 compared to controls
1/F	66	DM	1	3,969	negative	39.3
2/M	36	DM	3	2,815	positive	67.6
3/M	42	DM	2	1,163	negative	18.4
4/M	40	DM	12	70	negative	26.9
5/M	58	DM	1	3,006	negative	8.8
6/M	42	DM	2	＞6,000	negative	18.7
7/M	56	DM	1	3,034	negative	62.2
8/F	36	DM	6	＞6,000	negative	100.0
9/F	19	DM	3	668	not checked	122.0
10/F	51	PM	2	＞6,000	negative	75.4
11/M	25	PM	3	32	negative	21.8
12/F	62	PM	1	2,207	positive	139.0
13/F	34	PM	7	2,743	positive	218.0
14/F	43	PM	1	1,820	negative	10.5
	43.6±13.7		3.2±3.1	2,823±2,072		66.3±60.6

CK: creatinine kinase (normal＜160 units/liter), DM: dermatomyositis, PM: polymyositis

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