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Seo and Jeong: Effects of Blending Oil of Lavender and Thyme on Oxidative Stress, Immunity, and Skin Condition in Atopic Dermatitis Induced Mice
Original Article

Journal of Korean Academy of Nursing 2015; 45(3): 367-377.

Published online: 30 June 2015

DOI: https://doi.org/10.4040/jkan.2015.45.3.367

Effects of Blending Oil of Lavender and Thyme on Oxidative Stress, Immunity, and Skin Condition in Atopic Dermatitis Induced Mice

Young Mi Seo1, Seok Hee Jeong2

1Department of Nursing, Jeonju University, Jeonju, Korea.

2College of Nursing·Research Institute of Nursing Science, Chonbuk National University, Jeonju, Korea.

Address reprint requests to: Jeong, Seok Hee. College of Nursing, Chonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju 561-756, Korea. Tel: +82-63-270-3117, Fax: +82-63-270-3127, awesomeprof@jbnu.ac.kr

Received 12 November 2014       Revised 1 December 2014       Accepted 23 March 2015

#x00A9; 2015 Korean Society of Nursing Science

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution NoDerivs License. (http://creativecommons.org/licenses/by-nd/4.0/) If the original work is properly cited and retained without any modification or reproduction, it can be used and re-distributed in any format and medium.

Abstract

Purpose

The purpose of this study was to evaluate the effects of essential oil on oxidative stress, immunity, and skin condition in atopic dermatitis (AD) induced mice.

Methods

This study was a 3×3 factorial design. Factors were oil type (Lavender, Thyme, and 2:1 mixture of lavender and thyme oil [blending oil]) and treatment period (0 day, 7 days, and 21 days). The samples were 45 mice with AD and randomly assigned to nine groups of five mice per group. The dependent variables such as superoxide radical, IgE, degranulated mast cells, and epidermal thickness were measured. Data were collected from February to April in 2014. Descriptive statistics, One-way ANOVA, Two-way ANOVA, and Tukey's HSD test were performed using the SPSS WIN 20.0 program.

Results

Dependent variables were not statistically significantly different by the three oil types (p>.05). Essential oils such as lavender, thyme, and blending oil were all effective in reducing AD symptoms and especially 2:1 blending oil were most effective. There were statistically significant differences by the three treatment periods in all dependent variables (p<.001). There were statistically significant interactions between oil types and treatment periods in all dependent variables (p<.01). For decreasing superoxide radical, degranulated mast cells, and epidermal thickness, 2:1 mixed oil should be applied for at least 21 days. Otherwise to reduce IgE, 2:1 mixed oil should be used for at least 7 days.

Conclusion

These findings provide bases for developing effective interventions for AD patients to manage their AD symptoms.

Keywords: Atopic dermatitis, Essential oil, Superoxide radical, IgE, Mast cell

Figures and Tables

Figure 1

Measured variable on skin barrier.

jkan-45-367-g001
Figure 2

Photographs of skin in atopic dermatitis induced mice (1 to 9 number means the treatment period and oil type).

jkan-45-367-g002
Table 1

Comparison of Superoxide Radical, IgE, Degranulated Mast Cell, and Epidermal Thickness by Oil types and Treatment Period (N=45)

jkan-45-367-i001

*Tukey's HSD.

Table 2

Interactions between Oil Types and Treatment Periods (N=45)

jkan-45-367-i002

Notes

This manuscript is based on a part of the first author's doctoral dissertation from Chonbuk National University.

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