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Kim and Choe: Effects of Antioxidant on Reduction of Hindlimb Muscle Atrophy Induced by Cisplatin in Rats
Original Article

Journal of Korean Academy of Nursing 2014; 44(4): 371-380.

Published online: 29 August 2014

DOI: https://doi.org/10.4040/jkan.2014.44.4.371

Effects of Antioxidant on Reduction of Hindlimb Muscle Atrophy Induced by Cisplatin in Rats

Jin il Kim1, Myoung-Ae Choe2

1Department of Nursing, Christian College of Nursing, Gwangju, Korea.

2College of Nursing, Seoul National University, Seoul, Korea · Oita University of Nursing and Health Sciences, Oita, Japan.

Address reprint requests to: Kim, Jin il. Department of Nursing, Christian College of Nursing, 6, 70 beon-gil, Baekseo-ro, Nam-gu, Gwangju 503-711, Korea. Tel: +82-62-650-8057, Fax: +82-62-675-5806, neoreva@hanmail.net

Received 11 April 2014       Revised 27 April 2014       Accepted 23 June 2014

© 2014 Korean Society of Nursing Science

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution NoDerivs License. (http://creativecommons.org/licenses/by-nd/4.0/) If the original work is properly cited and retained without any modification or reproduction, it can be used and re-distributed in any format and medium.

Abstract

Purpose

The purpose of this study was to examine the effects of Cu/Zn SOD on reduction of hindlimb muscular atrophy induced by cisplatin in rats.

Methods

Forty-two rats were assigned to three groups; control group, Cisplatin (CDDP) group and cisplatin with Cu/Zn SOD (CDDP-SOD) group. At day 35 hindlimb muscles were dissected. Food intake, activity, withdrawal threshold, muscle weight, and Type I, II fiber cross-sectional area (CSA) of dissected muscles were measured. Relative SOD activity and expression of MHC and phosphorylated Akt, ERK were measured after dissection.

Results

Muscle weight and Type I, II fiber CSA of hindlimb muscles in the CDDP group were significantly less than the control group. Muscle weight and Type I, II fiber CSA of hindlimb muscles, food intake, activity, and withdrawal thresholds of the CDDP-SOD group were significantly greater than the CDDP group. There were no significant differences in relative SOD activities of hindlimb muscles between the CDDP-SOD and CDDP groups. MHC expression and phosphorylated Akt, ERK of hindlimb muscles in the CDDP-SOD group were significantly greater than the CDDP group.

Conclusion

Cu/Zn SOD attenuates hindlimb muscular atrophy induced by cisplatin through increased food intake and activity. Increment of phosphorylated Akt, ERK may relate to attenuation of hindlimb muscular atrophy.

Keywords: Antioxidant, Cisplatin, Rat, Muscular atrophy, Superoxide dismutase

Figures and Tables

Figure 1
Relative SOD activity of three groups.
jkan-44-371-g001
Figure 2
A. Expression of phosphorylated Akt, phosphorylated ERK and expression of MHC of soleus in C, CDDP and CDDP-SOD rats. B. Expression of phosphorylated Akt, phosphorylated ERK and expression of MHC of plantaris in C, CDDP and CDDP-SOD rats.
jkan-44-371-g002
Table 1
Body Weight and Mean Daily Diet Intake per Body Weight of C, CDDP and CDDP-SOD Rats
jkan-44-371-i001

*Kruskal-wallis test analysis; †Significant difference among three groups (p<.05); C=Control (n=14); CDDP=Cisplatin (n=14); CDDP-SOD=Cisplatin with superoxide dismutase (n=14).

Table 2
Mean Activity Score and Withdrawal Thresholds of C, CDDP and CDDP-SOD Rats
jkan-44-371-i002

*Kruskal-wallis test analysis; †Significant difference among three groups (p<.05); C=Control (n=14); CDDP=Cisplatin (n=14); CDDP-SOD=Cisplatin with superoxide dismutase (n=14).

Table 3
Weight of Hindlimb Muscles and Cross-Sectional Area of Hindlimb Muscles in C, CDDP and CDDP-SOD Rats
jkan-44-371-i003

*Significant difference between CDDP & C (p<.05); C=control (n=14); CDDP=cisplatin (n=14); CDDP-SOD=cisplatin with superoxide dismutase (n=14); CSA=cross-sectional area.

Notes

This article is a revision of the first author's doctoral thesis from Seoul National University.

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