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Choe and An: Effects of Nitric Oxide Synthase Inhibitor on Hindlimb Muscles in Rats with Neuropathic Pain Induced by Unilateral Peripheral Nerve Injury
Original Article
Journal of Korean Academy of Nursing

Journal of Korean Academy of Nursing 2011; 41(4): 520-527.

Published online: 31 August 2011

DOI: https://doi.org/10.4040/jkan.2011.41.4.520

Effects of Nitric Oxide Synthase Inhibitor on Hindlimb Muscles in Rats with Neuropathic Pain Induced by Unilateral Peripheral Nerve Injury

Myoung-Ae Choe1, Gyeong Ju An2

1Professor, College of Nursing, Seoul National University, Seoul, Korea.

2Assistant Professor, Department of Nursing, Cheongju University, Cheongju, Korea.

Address reprint requests to: An, Gyeong-Ju. Department of Nursing, Cheongju University, 586 Daeseong-ro, Sangdang-gu, Cheongju 360-764, Korea. Tel: +82-43-229-8992, Fax: +82-43-229-7988, antheresa@cju.ac.kr

Received 5 April 2011       Accepted 4 August 2011

© 2011 Korean Society of Nursing Science

Abstract

Purpose

The purpose of this study was to examine effects of nitric oxide synthase (NOS) inhibitor on muscle weight and myofibrillar protein content of affected and unaffected hindlimb muscles in rats with neuropathic pain induced by unilateral peripheral nerve injury.

Methods

Neuropathic pain was induced by ligation and cutting of the left L5 spinal nerve. Adult male Sprague-Dawley rats were randomly assigned to one of two groups: The NOSI group (n=19) had NOS inhibitor (L-NAME) injections daily for 14 days, and the Vehicle group (n=20) had vehicle injections daily for 14 days. Withdrawal threshold, body weight, food intake and activity were measured every day. At 15 days all rats were anesthetized and soleus, plantaris and gastrocnemius muscles were dissected from hindlimbs. Muscle weight and myofibrillar protein content of the dissected muscles were determined.

Results

The NOSI group showed significant increases as compared to the Vehicle group for body weight at 15 days, muscle weight and myofibrillar protein content of the unaffected soleus and gastrocnemius. The NOSI group demonstrated a higher pain threshold than the vehicle group.

Conclusion

NOSI for 14 days attenuates unaffected soleus and gastrocnemius muscle atrophy in neuropathic pain model.

Keywords: Pain, Nitric oxide synthase inhibitor, Muscular atrophy, Muscle proteins

Figures and Tables

Figure 1
Time course of changes in withdrawal threshold.
*p<.05.
NOSI=Nitric oxide synthase inhibitor.
jkan-41-520-g001
Table 1
Body Weight and Total Diet Intake of NOSI and Vehicle Rats
jkan-41-520-i001

NOSI=Nitric oxide synthase inhibitor.

Table 2
Mean Daily Activity Score of NOSI and Vehicle Rats
jkan-41-520-i002

NOSI=Nitric oxide synthase inhibitor.

Table 3
Muscle Weight of NOSI and Vehicle Rats
jkan-41-520-i003

NOSI=Nitric oxide synthase inhibitor.

Table 4
Myofibrillar Protein Content of the Hindlimb Muscles in NOSI and Vehicle Rats
jkan-41-520-i004

NOSI=Nitric oxide synthase inhibitor.

Notes

This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (R01-2007-000-10573-0).

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