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Choe and An: Effects of Exercise on Affected and Unaffected Hindlimb Muscles in Rats with Neuropathic Pain Induced by Unilateral Peripheral Nerve Injury
Original Article
Journal of Korean Academy of Nursing

Journal of Korean Academy of Nursing 2010; 40(5): 611-619.

Published online: 31 October 2010

DOI: https://doi.org/10.4040/jkan.2010.40.5.611

Effects of Exercise on Affected and Unaffected Hindlimb Muscles in Rats with Neuropathic Pain Induced by Unilateral Peripheral Nerve Injury

Myoung-Ae Choe1, Gyeong Ju An2

1Professor, College of Nursing, Seoul National University, Seoul, Korea.

2Professor, Department of Nursing, Cheongju University, Cheongju, Korea.

Address reprint requests to: Choe, Myoung-Ae. College of Nursing, Seoul National University, 28 Yeongeon-dong, Jongno-gu, Seoul 110-744, Korea. Tel: 82-2-740-8824, Fax: 82-2-765-4103, machoe@snu.ac.kr

Received 29 April 2010       Accepted 14 October 2010

Copyright © 2010 Korean Society of Nursing Science

Abstract

Purpose

The purpose of this study was to examine the effects of exercise on muscle weight and Type I and II fiber cross-sectional area of affected and unaffected hindlimb muscles in rats with neuropathic pain induced by unilateral peripheral nerve injury.

Methods

Neuropathic pain was induced by ligation and cutting of the left L5 spinal nerve. Adult male Sprague-Dawley rats were randomly assigned to one of two groups: The Pain+Exercise (PE) group (n=21) and the Sham+Exercise (SE) group (n=20). All rats had 28 sessions of treadmill exercise at grade 10 for 30 minutes, twice/day at 10 m/min for 14 days. Body weight, food intake and activity were measured every day. At 15 days all rats were anesthetized and soleus, plantaris and gastrocnemius muscles were dissected. Muscle weight and Type I, II fiber cross-sectional area of the dissected muscles were measured.

Results

The PE group showed significant increases (p<.05), as compared to the SE group for body weight and total diet intake, muscle weight of the unaffected soleus and plantaris, and in Type I and II fiber cross-sectional area of unaffected three muscles and affected plantaris.

Conclusion

Exercise for 14 days attenuates unaffected soleus, plantaris and gastrocnemius muscle atrophy in neuropathic pain model.

Keywords: Pain, Exercise, Muscular atrophy, Muscle weight, Muscle fiber

Figures and Tables

Figure 1
Cross-section of the hindlimb muscles in Pain+Exercise and Sham+Exercise rats. The first line is the affected soleus (left), plantaris (middle) and gastrocnemius muscle (right) in Pain+Exercise rats. The second line is the unaffected soleus (left), plantaris (middle) and gastrocnemius muscle (right) in Pain+Exercise rats. The third line is the affected soleus (left), plantaris (middle) and gastrocnemius muscle (right) in Sham+Exercise rats. The fourth line is the unaffected soleus (left), plantaris (middle) and gastrocnemius muscle (right) in Sham+Exercise rats. Dark=Type I muscle fiber, light=Type II muscle fiber (Myosin ATPase straining, 100×).
jkan-40-611-g001
Table 1
Body Weight and Total Diet Intake of Pain+Exercise and Sham+Exercise Rats
jkan-40-611-i001
Table 2
Total Activity Score of Pain+Exercise and Sham+Exercise Rats
jkan-40-611-i002
Table 3
Muscle Weight of Pain+Exercise and Sham+Exercise Rats
jkan-40-611-i003
Table 4
Cross-Sectional Area of the Hindlimb Muscles in Pain+Exercise and Sham+Exercise Rats
jkan-40-611-i004

Notes

This work was supported by Mid-career Researcher Program through NRF grant funded by the MEST (2009-0053460).

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