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Kim and Choe: Effect of Decreased Locomotor Activity on Hindlimb Muscles in a Rat Model of Parkinson's Disease
Original Article
Journal of Korean Academy of Nursing

Journal of Korean Academy of Nursing 2010; 40(4): 580-588.

Published online: 31 August 2010

DOI: https://doi.org/10.4040/jkan.2010.40.4.580

Effect of Decreased Locomotor Activity on Hindlimb Muscles in a Rat Model of Parkinson's Disease

Yongbum Kim1, Myoung-Ae Choe2

1Student, Bredin Institute, Edmonton, AB, Canada.

2Professor, College of Nursing, Seoul National University, Seoul, Korea.

Address reprint requests to: Choe, Myoung-Ae. College of Nursing, Seoul National University, 28 Yeongeon-dong, Jongno-gu, Seoul 110-799, Korea. Tel: 82-2-740-8824, Fax: 82-2-765-4103, machoe@snu.ac.kr

Received 19 April 2010       Accepted 16 August 2010

Copyright © 2010 Korean Society of Nursing Science

Abstract

Purpose

The purpose of this study was to examine effects of decreased locomotor activity on mass, Type I and II fiber cross-sectional areas of ipsilateral and contralateral hindlimb muscles 21 days after establishing the Parkinson's disease rat model.

Methods

The rat model was established by direct injection of 6-hydroxydopamine (6-OHDA, 50 µg) into the left substantia nigra after stereotaxic surgery. Adult male Sprague-Dawley rats were assigned to one of two groups; the Parkinson's disease group (PD; n=17) and a sham group (S; n=8). Locomotor activity was assessed before and 21 days after the experiment. At 22 days after establishing the rat model, all rats were anesthetized and soleus and plantaris muscles were dissected from both ipsilateral and contralateral sides. The brain was dissected to identify dopaminergic neuronal death of substantia nigra in the PD group.

Results

The PD group at 21 days after establishing the Parkinson's disease rat model showed significant decrease in locomotor activity compared with the S group. Weights and Type I and II fiber cross-sectional areas of the contralateral soleus muscle of the PD group were significantly lower than those of the S group.

Conclusion

Contralateral soleus muscle atrophy occurs 21 days after establishing the Parkinson's disease rat model.

Keywords: Parkinson's disease, Rats, Hindlimb, Muscle atrophy

Figures and Tables

Figure 1
Experimental design.
jkan-40-580-g001
Figure 2
TH-specific immunohistochemical staining in substantia nigra in unilateral 6-OHDA lesioned rats.
jkan-40-580-g002
Table 1
Preweight and Postweight between the Groups
jkan-40-580-i001

n=number of animals.

PD=Parkinson's disease; S=sham.

Table 2
Total Dietary Intake between the Groups
jkan-40-580-i002

n=number of animals.

PD=Parkinson's disease; S=sham.

Table 3
Locomotor Activity of Parkinson's Disease (PD) and Sham (S) Groups
jkan-40-580-i003

n=number of animals.

PD=Parkinson's disease; S=sham.

Table 4
Muscle Weight of Hindlimb Muscles of Parkinson's Disease (PD) and Sham (S) Groups
jkan-40-580-i004

n=number of animals.

PD=Parkinson's disease; S=sham.

Table 5
Cross-Sectional Area of Hindlimb Muscles of Parkinson's Disease (PD) and Sham (S) Groups
jkan-40-580-i005

n=number of animals.

PD=Parkinson's disease; S=sham.

Notes

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (810-20090016).

This article is a part of master's thesis.

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