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Kim: An Association between 609 C → T Polymorphism in NAD(P)H: Quinone Oxidoreductase 1 (NQO1) Gene and Blood Glucose Levels in Korean Population
Original Article
Korean Diabetes Journal

Korean Diabetes Journal 2009; 33(1): 24-30.

Published online: 28 February 2009

DOI: https://doi.org/10.4093/kdj.2009.33.1.24

An Association between 609 C → T Polymorphism in NAD(P)H: Quinone Oxidoreductase 1 (NQO1) Gene and Blood Glucose Levels in Korean Population

Dohee Kim

Department of Internal Medicine, Dankook University College of Medicine, Cheonan, Korea.

dh9070@hanmail.net

Received 2 February 2009       Accepted 14 February 2009

Copyright © 2009 Korean Diabetes Association

Abstract

Background

NAD(P)H: quinone oxidoreductase 1 (NQO1), which is an obligate two-electron reductase that utilizes NAD(P)H as an electron donor and is involved in the protection against oxidative stress, is likely involved in β-cell destruction. We evaluated the frequency of the NQO1 polymorphism and its association with blood glucose levels.

Methods

Genotypes were determined using a polymerase chain reaction restriction fragment length polymorphism-based assay in 56 patients and 48 healthy subjects. Fasting blood glucose, insulin, and lipid profiles were measured and homeostasis model assessment (HOMA)-insulin resistance (IR) was calculated from fasting glucose and insulin levels in the healthy subjects.

Results

The genotype frequencies of NQO1 polymorphism were C/C (56.7%), C/T (42.3%), and T/T (1.0%). There were no associations between the NQO1 polymorphism and body mass index, blood pressure, lipid profile, HbA1c, postprandial glucose, and HOMA-IR. However, NQO1 mutants (C/T and T/T) showed weak but significantly higher fasting blood glucose levels compared with wild type (C/C).

Conclusion

Our data suggest that NQO1 609 C → T polymorphism may be associated with glucose metabolism.

Keywords: Blood glucose, Human NQO1, Single nucleotide polymorphism

Figures and Tables

Fig. 1
The distributions of genotypes in subjects. A. Total. B. Men. C. Women.
kdj-33-24-g001
Table 1
The relationship between NQO1 genetic polymorphism and metabolic parameters
kdj-33-24-i001

Data are expressed as means ± S.D. *P < 0.05 vs. wild type. BP, blood pressure; HDL, high density lipoprotein; LDL, low density lipoprotein.

Table 2
The relationship between NQO1 genetic polymorphism and HOMA-IR
kdj-33-24-i002

Data are expressed as means ± S.D. HOMA-IR, homeostasis model assessment-insulin resistance.

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