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Kim, Choi, Song, Lee, Lee, Oak, Jang, Jung, and Jang: Comparison of Forcep-biopsy and Cryo-biopsy by a Flexible Bronchoscopy
Original Article
Tuberculosis and Respiratory Diseases

Tuberculosis and Respiratory Diseases 2009; 66(2): 110-115.

Published online: 28 February 2009

DOI: https://doi.org/10.4046/trd.2009.66.2.110

Comparison of Forcep-biopsy and Cryo-biopsy by a Flexible Bronchoscopy

Jae Hyun Kim, M.D.1, Jung Min Choi, M.D.1, Sung Eun Song, M.D.1, Eun Mi Lee, M.D.1, Song Ju Lee, M.D.1, Chul Ho Oak, M.D.1, Tae Won Jang, M.D.1, Man Hong Jung, M.D.1, Hee Kyung Jang, M.D.2

1Department of Internal Medicine, Kosin University College of Medicine, Busan, Korea.

2Department of Pathology, Kosin University College of Medicine, Busan, Korea.

Address for correspondence: Chul Ho Oak, M.D. Department of Internal Medicine, College of Medicine, Kosin University, 34, Amnam-dong, Seo-gu, Busan 602-702, Korea. Phone: 82-51-990-6104, Fax: 82-51-990-3049, oaks70@hanmail.net

Received 15 December 2008       Accepted 22 January 2009

Copyright©2009. The Korean Academy of Tuberculosis and Respiratory Diseases. All rights reserved.

Abstract

Background

A forceps-biopsy is performed to acquire tissue from patients with an endobronchial carcinoma using a flexible bronchoscope. Recently, a cryo-biopsy has also been used to acquire tissue samples. Cryo-biopsy is the diagnostic application of extreme cold for the local destruction of abnormal living tissue. This technique is safe, with no radiation danger, no risk of electrical accidents, and a little risk of bleeding. This study compared a forceps-biopsy with a cryo-biopsy using a flexible bronchoscope, and examined the chemosensitivity and level of VEGF (vascular endothelial growth factor) in the specimens obtained from the cryo-biopsy.

Methods

We present a prospective study of 30 consecutive patients who underwent a forceps-biopsy between January 2007 and October 2007 with a mean age of 62.1 years and a male:female ratio of 5 : 1. A flexible bronchoscope was inserted to the area of the abnormal lesions, and a cryo-probe was then applied through the working channel of the flexible bronchoscope. A temperature of approximately -80 was delivered to the tumor site for 8 seconds. The cryo-biopsy was performed after destroying the tumor mass.

Results

The mean size of the tissue from the forceps-biopsy and cryo-biopsy were 2.0±1.2 mm and 6.0±3.0 mm. A chemosensitivity test was performed on 5 specimens obtained using cryo-biopsy and the level of VEGF was examined in 2 specimens obtained from a cryo-biopsy. There were no side effects in either group.

Conclusion

Cryo-biopsy using a flexible bronchoscope is a safe and effective technique for acquiring tissue samples.

Keywords: Forcep-biopsy, Cryo-biopsy, Endobronchial carcinoma

Figures and Tables

Figure 1
Procedure of the cryo-biopsy.
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Figure 2
Comparison of microscopic findings from bronchial specimens obtained by forcep biopsy (A, size 2.0±1.2 mm) and cryo biopsy (B, size 6.0±3.0 mm) (H&E stain, ×400).
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Figure 3
Vascular Endothelial Growth Factor (VEGF) expression (arrows) in cryo biopsy (VEGF stain, ×400).
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Table 1
Characteristics of patients
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Table 2
Characteristics of cryobiopsy
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Table 3
Comparison of the time (of procedure) and specimen profiles between forcep biopsy and cryobiopsy
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