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Abstract
Background
This study examined the role of neutrophilc oxidative stress in an O2-induced acute lung injury (ALI).
Methods
For 48 h, experimental rats were exposed to pure oxygen (normobaric hyperoxia) in a plastic cage. Forty-eight hours after O2 breathing, the rats were sacrificed and the parameters for ALI associated with neutrophilic oxidative stress were assessed.
Results
Normobaric pure oxygen induced ALI, which was quite similar to ARDS. The O2-induced neutrophilic oxidative stress was identified by confirming of the increase in lung myeloperoxidase, BAL neutrophils, malondialdehyde (MDA), cytosolic phospholipase A2 (cPLA2) activity in the lung, histological changes and BAL cytospin morphology.
Figures and Tables
Figure 1
(A) The representative photograph of BALF cytospin of rat given ambient air. Almost all of the cells are monocytes (Wright's stain, ×100). (B) The representative photograph of BALF cytospin of rats given 100% O2 for 48 h. The intermingling of monocytes and neutrophils is noted. Neutrophils are much more abundant than monocytes (Wright's stain, ×40).
Figure 2
(A) The normal histological finding of the lung of rat given ambient air. Patent alveoli and thin, normal alveolar septa are noted (H&E stain, ×100). (B) The histological finding of the lung given 100% O2 for 48 h. Diffuse hemorrhage is noted. Intraalveolar hemorrhage, migrated phagocytes and hyaline membranes are shown (H&E stain, ×40). (C) The photograph of the migration of phagocytes from vascular lumen into alveolar lumen in the lung of rats given 100% O2 for 48 h. Phagocytes including neutrophils are trapped in the perivascular cuff (below right) and phagocytes are migrating into alveolar lumen from adjacent vessel. Extravasation of the red blood cells are noted also (H&E stain, ×100). (D) The magnification of Figure C. Note the migration of phagocytes from vessel into alveolar lumen. Neutrophils are moving into the alveolar lumen by a diapedesis and red blood cells are migrating into the alveolar lumen also (H&E stain, ×400).
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