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Lee, Cho, Kim, Park, Kim, Lee, Kim, Jeon, Park, Lee, and Park: Relations between Polymorphism of NRAMP1 Gene and Susceptibility to Pulmonary Tuberculosis
Original Article
Tuberculosis and Respiratory Diseases

Tuberculosis and Respiratory Diseases 2007; 62(6): 492-498.

Published online: 30 June 2007

DOI: https://doi.org/10.4046/trd.2007.62.6.492

Relations between Polymorphism of NRAMP1 Gene and Susceptibility to Pulmonary Tuberculosis

Ji Seok Lee, M.D.1, Jin Hoon Cho, M.D.1, Ki Uk Kim, M.D.1, Hye-Kyung Park, M.D.1, Yun Seong Kim, M.D.1, Ho Seok Lee, M.D.2, Young Dae Kim, M.D.2, Doo Soo Jeon, M.D.3, Seung Kyu Park, M.D.3, Min Ki Lee, M.D.1, Soon Kew Park, M.D.1

1Department of Internal Medicine, College of Medicine, Pusan National University, Busan, Korea.

2Department of Thoracic Surgery, College of Medicine, Pusan National University, Busan, Korea.

3Masan National Hospital, Masan, Korea.

Address for correspondence: Min Ki Lee, M.D. Departments of Internal Medicine, College of Medicine, Pusan National University, 1-10 Ami-dong, Suh-gu, Busan 602-739, Korea. Phone: 82-51-240-7216, Fax: 82-51-254-3127, leemk@pusan.ac.kr

Received 20 April 2007       Accepted 28 May 2007

Copyright © 2007 The Korean Academy of Tuberculosis and Respiratory Diseases

Abstract

Background

Several lines of evidence suggest that a host's genetic factors influence the outcome of exposure to Mycobacterium tuberculosis. The aim of this study was to determine whether polymorphism in NRAMP1 (natural resistance associated macrophage protein 1) gene is associated with the susceptibility or resistance to tuberculosis infection for patients with drug-sensitive pulmonary tuberculosis (DS-TB) and multi-drug resistant pulmonary tuberculosis (MDR-TB).

Methods

Eight genetic polymorphisms of the NRAMP1 gene were investigated in patients suffering with DS-TB (n=100) or MDR-TB (n=102), and in healthy normal controls (NC, n=96). The genetic polymorphisms of NRAMP1 were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).

Results

The frequency of D543N A/G heterogygotes was significantly higher in the DS-TB subjects than the NCs (OR=2.10, 95% CI: 1.00 to 4.41, p=0.049). The frequency of 823C/T T/C heterozygotes was significantly higher in the DS-TB subjects (OR=2.79, 95% CI: 1.11 to 7.04, p=0.029) and the MDR-TB subject (OR=3.30, 95% CI 1.33 to 8.18, p=0.010) than in the NCs. However, the frequency of these genotypes was not different between the DS-TB and MDR-TB subjects.

Conclusion

A significant association was found between NRAMP1 823 C/T polymorphism and pulmonary tuberculosis. This result suggests that NRAMP1 polymorphism may be involved in the development of pulmonary tuberculosis in Koreans.

Keywords: NRAMP1, Polymorphism, Tuberculosis

Figures and Tables

Table 1
Identification of NRAMP1 polymorphisms
trd-62-492-i001
Table 2
Demographic characteristics of subjects
trd-62-492-i002

DS-TBL: Drug sensitive tuberculosis; MDR-TB: Multi-drug resistant tuberculosis.

*Data are Mean ± standard deviation.

Table 3
Genotype frequencies of NRAMP1
trd-62-492-i003

NC: Normal control; DS-Tb: Dug sensitive tuberculosis; MDR-Tb: Multi drug resistance tuberculosis; OR: Odds ratio; CI: Confidence interval. NS: Not significant p>0.05.

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