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Choi, Shin, Kim, Kim, and Lee: Usefulness of Automated PCR Test for the Detection of Mycobacterium Tuberculosis in Fresh Biopsy Tissues
Original Article
Tuberculosis and Respiratory Diseases

Tuberculosis and Respiratory Diseases 2006; 61(1): 54-59.

Published online: 31 July 2006

DOI: https://doi.org/10.4046/trd.2006.61.1.54

Usefulness of Automated PCR Test for the Detection of Mycobacterium Tuberculosis in Fresh Biopsy Tissues

Woo Soon Choi, M.T.1, So Young Shin, M.D.1, Jong Ok Kim, M.D.2, Myung Sook Kim, M.D.3, Hye Kyung Lee, M.D.2

1Department of Laboratory Medicine, College of Medicine, The Catholic University, Daejeon, Korea.

2Department of Pathology, College of Medicine, The Catholic University, Daejeon, Korea.

3Department of Internal Medicine, College of Medicine, The Catholic University, Daejeon, Korea.

Address for correspondence: Hye Kyung Lee, M.D. Department of Pathology, University of Catholic University College of Medicine, Daejeon St. Mary's Hospital, 520-2 Daehung-dong, Jung-Gu, Daejeon 301-723, Korea. Phone: 042-220-9612, Fax: 042-220-9915, apw01@hanmail.net

Received 11 May 2006       Accepted 4 July 2006

Copyright © 2006 The Korean Academy of Tuberculosis and Respiratory Diseases

Abstract

Background

Although there have been several studies regarding the clinical value of an automated TB-PCR study using sputum, bronchial washing, and other body fluid samples for the detection of pulmonary tuberculosis, there are only a few reports on the use of fresh tissue samples.

Materials and methods

The acid-fast bacilli stain(AFB), tuberculosis culture, automated TB-PCR study, and histopathology examination were performed in 42 fresh tissue samples.

Results

Among the 42 cases, 18 cases were diagnosed with tuberculosis based on the clinical findings. Sixteen of the 18 cases were TB-PCR positive and of these 16 cases, only 2 cases were positive in the AFB stain or culture study. However, all 18 cases showed the histopathology findings of chronic granulomatous inflammation that was compatible with tuberculosis. Based on the clinical findings, the sensitivity, specificity, positive predictability, and negative predictability of the automated TB-PCR study were 88.9%, 100%, 100%, and 92.3% respectively.

Conclusion

An automated TB-PCR assay is an important diagnostic tool for diagnosing tuberculosis in fresh tissue samples.

Keywords: Fresh tissue sample, Automated tuberculosis polymerase chain reaction(TB-PCR)

Figures and Tables

Table 1
Type and number of fresh tissues according to TB-PCR positivity
trd-61-54-i001
Table 2
The results of the TB-PCR test in fresh tissues
trd-61-54-i002
Table 3
The results of TB-PCR test according to final diagnosis
trd-61-54-i003
Table 4
The results of the AFB smear, culture, TB-PCR test histopathology in fresh tissues
trd-61-54-i004

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