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Park, Kang, Lim, Ha, Cho, Lee, Choi, Choi, Sheen, and Bai: Analysis of DNA fingerprints of Mycobacterium Tuberculosis Isolates from Patients Registered at Health Center in Gyeonggi Province in 2004
Original Article
Tuberculosis and Respiratory Diseases

Tuberculosis and Respiratory Diseases 2006; 60(3): 290-296.

Published online: 31 March 2006

DOI: https://doi.org/10.4046/trd.2006.60.3.290

Analysis of DNA fingerprints of Mycobacterium Tuberculosis Isolates from Patients Registered at Health Center in Gyeonggi Province in 2004

Young Kil Park1, Hee Yeun Kang1, Jang Geun Lim1, Jong Sik Ha1, Jung Ok Cho2, Ka Chel Lee2, Hang Soon Choi2, Young Hwa Choi3, Seung Soo Sheen3, Gill Han Bai1

1Korean Institute of Tuberculosis, Seoul, Korea.

2Hygiene Policy Department, Gyeonggi Provincial Government, Gyeonggi Province, Korea.

3Ajou University School of Medicine, Gyeonggi Province, Korea.

Address for correspondence: Gill Han Bai, Ph.D. Korean Institute of Tuberculosis 14 Woomyundong, Sochogu, Seoul. Phone: 02-576-4981, Fax: 02-573-1914, gbai@hotmail.com

Received 17 January 2006       Accepted 13 March 2006

Copyright © 2006 The Korean Academy of Tuberculosis and Respiratory Diseases

Abstract

Background

IS6110 DNA fingerprint is a very useful tool for investigating the transmission of tuberculosis. The aim of this study was to identify the epidemiological situations within a given area (one province).

Methods

The 681 Mycbobacterium tuberculosis isolates from patients, who were registered at health centers in Gyeonggi Province from May to December in 2004, were subjected to IS6110 DNA fingerprinting. Patients belonging to clusters were interviewed by health-workers to determine their previous contacts or household TB history.

Results

The number of IS6110 copies of the 681 isolates showed diverse fingerprint patterns from 0 to 21 of which the most prevalent copy number was 10 from 120 isolates (17.6%). Thirty-three isolates (4.8%) belonged to the K strain, and 128 isolates (18.8%) belonged to the K family. There were 180 (26.4%) isolates belonged belonging to fifty clusters, of which two clusters were within household transmission. Forty-three (23.9%) out of 180 patients resided in an area under the same health center control. The rate of clusters in those aged 60-70 was higher than in any other age group ( 95% CI of RR : 1.072 ~ 1.988).

Conclusion

This is the first report of an epidemiological survey based on a whole province using a DNA fingerprinting technique for M. tuberculosis. These results will be helpful in developing a program or policies to prevent the transmission of TB.

Keywords: Mycbobacterium tuberculosis, DNA fingerprinting, IS6110, Gyeonggi Province, Epidemiology, Cluster

Figures and Tables

Figure 1
Number of M. tuberculosis isolates by IS6110 copies
trd-60-290-g001
Table 1
Cluster distribution by age and sex
trd-60-290-i001
Table 2
Statistical analysis of cluster rates by sex and age group
trd-60-290-i002

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