Journal List > Tuberc Respir Dis > v.57(3) > 1000688

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Choi, Lee, Song, Lim, Son, Lim, Choi, Kim, and Kim: Association between RASSF1A Methylation and Clinicopathological Factors in Patients with Squamous Cell Carcinoma of Lung
Original Article

Tuberculosis and Respiratory Diseases 2004; 57(3): 265-272.

Published online: 30 September 2004

DOI: https://doi.org/10.4046/trd.2004.57.3.265

Association between RASSF1A Methylation and Clinicopathological Factors in Patients with Squamous Cell Carcinoma of Lung

Naeyun Choi, M.S.1, Hye-Sook Lee, Ph.D.1, In Seung Song, B.S.1, Yu Sung Lim, B.S.1, Dae-Soon Son, M.S.1, Dae-Sik Lim, Ph.D.4, Yong Soo Choi, M.D.3, Jhingook Kim, M.D.3, Hojoong Kim, M.D.2

1Cancer Research Center, Center for Clinical Research, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Seoul 135-710, Korea.

2Division of Pulmonary and Critical Care Medicine, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 135-710, Korea.

3Department of Thoracic Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 135-710, Korea.

4Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseoung-D, Yuseong-G, Daejeon 305-701, Korea.

Address for correspondence: Hojoong Kim, M.D. Division of Pulmonary and Critical Care Medicine, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 135-710, Korea. Phone: 02-3410-3429, Fax: 02-3410-3489, hjkim@smc.samsung.co.kr

Received 3 March 2004       Accepted 7 July 2004

Copyright©2004. The Korean Academy of Tuberculosis and Respiratory Diseases. All rights reserved.

Abstract

Background

RASSF1A, which is one of tumor suppressor genes, is frequently inactivated by hypermethylation of the promoter region in a variety of human cancers, including lung cancer. This study was performed to investigate the association between RASSF1A methylation and the clinicopathological factors in patients with squamous cell carcinoma of the lung.

Methods

Eighty-one samples from the patients with squamous cell carcinoma of lung were examined. The promoter methyation of RASSF1A was analyzed by methylation specific PCR and sequencing. Statistical analysis was made to examine the association between RASSF1A methylation and the clinicopathological parameters.

Results

RASSF1A methylation was observed in 37.0% (30 of 81) of the patients with squamous cell carcinoma of the lung. RASSF1A methylation was found to be associated with cellular differentiation(p=0.0097) and the overall survival(p=0.0635). However, there was no association between RASSF1A methylation and the other clinicopathological parameters, such as the pathological TNM stage, the recurrence rate, lymph node invasion and the amount of cigarettes smoked.

Conclusion

RASSF1A methylation might be associated with a poor prognosis in patients with squamous carcinoma of the lung. A larger scale study is needed.

Keywords: Methylation, Squamous cell carcinoma, Tumor suppressor gene

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