Journal List > Tuberc Respir Dis > v.57(1) > 1000685

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Park, Kim, Jee, and Lee: IFN-γmRNA Expression in Tuberculous Pleural Lymphocytes After in vitro Stimulation with M. tuberculosis Antigens
Original Article

Tuberculosis and Respiratory Diseases 2004; 57(1): 25-31.

Published online: 31 July 2004

DOI: https://doi.org/10.4046/trd.2004.57.1.25

IFN-γmRNA Expression in Tuberculous Pleural Lymphocytes After in vitro Stimulation with M. tuberculosis Antigens

Jae Seuk Park, M.D., Youn Seup Kim, M.D., Young Koo Jee, M.D., Kye Young Lee, M.D.

Department of Internal Medicine, Dankook University College of Medicine, Chonan, Korea.

Address for correspondence: Kye Young Lee, M.D. Department of Internal Medicine, Dankook University College of Medicine 16-5, Anseo-dong Chonan, 330-715, Korea. Phone: 041-550-3916, Fax: 041-556-3256, kyleemd@dankook.ac.kr

Received 18 February 2003       Accepted 27 May 2004

Copyright©2004. The Korean Academy of Tuberculosis and Respiratory Diseases. All rights reserved.

Abstract

Background

IFN-γ is the main effector mediator of the host immune response against Mycobacterium tuberculosis. Evaluating the IFN-γ gene expression in response to M. tuberculosis antigens may help in elucidating the host defense mechanism against M. tuberculosis and in the development of a vaccine.

Methods

The IFN-γ mRNA expression in the lymphocytes obtained from pleural effusions from tuberculous pleurisy patients (TB-PLC) after in vitro stimulation with whole cell M. tuberculosis(H37Rv), purified protein derivatives(PPD), man-lipoarabinamman (man-LAM), ara-LAM and Antigen 85B(Ag85B) were evaluated. The degree of IFN-γ mRNA expression was determined by a semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) method.

Results

M. tuberculosis induced the expression of IFN-γ mRNA in the TB-PLC in time and dose dependent manners. The PPD and Ag85B induced high levels of IFN-γ mRNA expression in the TB-PLC. However, man-LAM inhibited IFN-γ mRNA expression in the TB-PLC, while ara-LAM did not.

Conclusion

IFN-γ mRNA expression in TB-PLC is stimulated by PPD and Ag85B, but inhibited by man-LAM.

Keywords: Interferon-γ, Tuberculosis, PPD, Ag85B, LAM

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