Journal List > Tuberc Respir Dis > v.57(2) > 1000668

TOOLS
Lee, Jeon, Park, Lee, Cho, Kim, and Bai: Construction of Recombinant BCGs Overexpressing Antigen 85 Complex and Their Protective Efficacy against Mycobacterium tuberculosis Infection in a Mouse Model
Original Article

Tuberculosis and Respiratory Diseases 2004; 57(2): 125-131.

Published online: 31 August 2004

DOI: https://doi.org/10.4046/trd.2004.57.2.125

Construction of Recombinant BCGs Overexpressing Antigen 85 Complex and Their Protective Efficacy against Mycobacterium tuberculosis Infection in a Mouse Model

Seung Heon Lee1, Bo Young Jeon2, Young Gil Park1, Hye Young Lee3, Sang Nae Cho2, Hyo Joon Kim4, Gill Han Bai1

1Department of Molecular Biology, Korean Institute of Tuberculosis, Seoul, Korea.

2Department of Microbiology, Yonsei University College of Medicine, Seoul, Korea.

3Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Korea.

4Department of Biochemistry and Molecular Biology, College of Science Technology, Hanyang University, Ansan, Korea.

Address for correspondence: Gill-Han Bai. Korean Institute of Tuberculosis 14 Woomyundong, Sochogu, Seoul, 137-140, Korea. Phone: 02-577-5766, Fax: 02-573-1914, gbai@hotmail.com

Received 26 March 2004       Accepted 14 May 2004

Copyright©2004. The Korean Academy of Tuberculosis and Respiratory Diseases. All rights reserved.

Abstract

Tuberculosis (TB) remains an enormous global health problem, and a new vaccine against TB more potent than the current inadequate BCG vaccine is urgently needed. We constructed three recombinant Mycobacterium bovis BCG (rBCG) strains over-expressing antigen (Ag) 85A, Ag85B, or both of M. tuberculosis using their own promoter and secretory sequence, or hsp60 promoter. SDS-PAGE analysis of rBCG proteins showed over-expression of Ag85A and Ag85B proteins in higher level than of those in their parental strain of BCG. In addition, rBCG(rBCG/B.FA) over-expressing Ag85A and Ag85B induced strong IFN-γ production in splenocytes. However, there was no significant difference in protective efficacy between rBCG and their parental BCG strain. In this study, therefore, rBCG over-expressing Ag85A, Ag85B, or both failed to show enhanced protection against M. tuberculosis infection in a mouse model.

Keywords: Tuberculosis, Vaccine, Recombinant BCG

TOOLS
Similar articles