Comparison of Group A, B and C Rotaviral Gastroenteritis among Children in Korea: Prevalence and Clinical Features

Kil-Seong Bae; Woo Ri Bae; Ji Hoon Kim; Joong Hyun Bin; Hyun Hee Kim; Hee Jin Lee; Wonbae Lee

doi:10.14776/kjpid.2014.21.2.96

Journal List > Korean J Pediatr Infect Dis > v.21(2) > 1096437

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Bae, Bae, Kim, Bin, Kim, Lee, and Lee: Comparison of Group A, B and C Rotaviral Gastroenteritis among Children in Korea: Prevalence and Clinical Features

Original Article

Korean J Pediatr Infect Dis 2014;21(2):96-103.

Published online: 20 January 2014

DOI: https://doi.org/10.14776/kjpid.2014.21.2.96

Comparison of Group A, B and C Rotaviral Gastroenteritis among Children in Korea: Prevalence and Clinical Features

Kil-Seong Bae, M.D.^∗, Woo Ri Bae, M.D.^∗, Ji Hoon Kim, M.D.^∗, Joong Hyun Bin, M.D.^∗, Hyun Hee Kim, M.D.^∗, Hee Jin Lee, M.D.^†, Wonbae Lee, M.D.^∗

^∗Department of Pediatrics, The Catholic University of Korea, Bucheon St Mary' s Hospital, Bucheon, Korea

^†Clinical Medical Research Institute, The Catholic University of Korea, Bucheon St Mary' s Hospital, Bucheon, Korea

Correspondence: Wonbae Lee, M.D. Department of Pediatrics, the Catholic University of Korea, Bucheon St. Mary' s Hospital, South Korea Tel: +82-32-340-7045, Fax: 82-32-340-2673 E-mail: leewb@catholic.ac.kr

Received 15 August 2014 Revised 17 February 2014 Accepted 9 June 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose

The aim of this study is that the prevalence of rotavirus infection was evaluated by each group and clinical features of group A, B and C rotaviruses infections were described respectively to compare one with another. Methods: Between January 2010 and December 2010, we enrolled a group of children below 10 years of age admitted for management of acute diarrhea at the Catholic University of Korea Bucheon St. Mary's Hospital. A total of 310 stool samples documented to be free of common bacterial pathogens were collected from children with diarrhea. The presence of group A, B or C rotavirus is indicated by amplification of DNA segments of the expected lengths after the first and second PCRs Results: In a total of 310 stool specimens, 40 (12.9%) specimens were positive for rotaviruses. These included 23 (7.4%) positive for group A, 5 (1.6%) for group B and 12 (3.9%) for group C rotaviruses. Group B rotavirus infected patients had significantly less diarrheas per day (group A: P=0.01, group C: P=0.01) and shorter duration of vomiting days (group A: P=0.03, group C: P=0.03) than those with group A and C rotaviruses infection respectively. All the group B rotaviruses had been isolated in March and October. Group C rotavirus infections were prevalent during late summer and early winter and peaked in October. Conclusion: These findings indicate that group B and C rotaviruses are notable causes or the contributing causes of diarrhea among infants and children in Korea.

Keywords: Rotavirus, Gastroenteritis, Prevalence

References

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Fig. 1.

PCR primers for detection of rotavirus groups A, B and C.

Fig. 2.

Monthly distribution of group A, B and C rotavirus-positive cases in Korea.

Table 1.

Primers used for RT-PCR

Primer (nested)	Name	Oligonucleotide Sequence (5'-3')
Rotavirus A (VP7)	A1	GGC TTT AAA AGA GAG AAT TTC CGT CTG G(forward)
	A2	GGA CCA AGA GAA AAC GTA GC (reverse)
	A4	GGT CAC ATC ATA CAA TTC TAA TCT AAG (reverse)
Rotavirus B (VP7)	B1	CTA TTC AGT GTG TCG TGA GAG G (forward)
	B3	CGA AGC GGG CTA GCT TGT CTG C (reverse)
	B4	CGT GGC TTT GGA AAA TTC TTG (reverse)
Rotavirus C (VP6)	C1	CTC GAT GCT ACT ACA GAA TCA G (forward)
	C3	GGG ATC ATC CAC GTC ATG CG (reverse)
	C4	AGC CAC ATA GTT CAC ATT TCA TCC (reverse)

Table 2.

Comparison of Clinical Features of Patients Infected by Group A, B and C Rotaviruses

	Group A (N=23)		Group B (N=5)		Group C (N=12)		P-value
	Median	Range	Median	Range	Median	Range	P-value
Age (months)	12	2–72	14	6–36	17	4–78	>0.05
Hospitalization (days)	6	2–11	6	3–7	5	1–11	>0.05
Diarrhea duration (days)	5	3–9	3	2–5	4	2–9	>0.05
Diarrhea frequency (/day)	9	3–20	4	2–6	6	3–8	0.01
Vomiting duration (days)	2	1–3	0	0–1	2	0–3	0.03
Vomiting frequency (/day)	4	2–10	0	0–3	3	0–6	>0.05
Fever (days)	2	0–6	0	0–4	2	0–5	>0.05

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