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Huh, Hong, Kim, Lim, and Koh: The Efficacy of α-lipoic Acid on the Endotoxin-induced Acute Lung Injury
Original Article
Tuberculosis and Respiratory Diseases

Tuberculosis and Respiratory Diseases 2007; 62(2): 105-112.

Published online: 28 February 2007

DOI: https://doi.org/10.4046/trd.2007.62.2.105

The Efficacy of α-lipoic Acid on the Endotoxin-induced Acute Lung Injury

Jin Won Huh, M.D.1, Sang Bum Hong, M.D.2, Mi Jung Kim, M.D.3, Chae-Man Lim, M.D.2, Younsuck Koh, M.D.2

1Department of Internal Medicine, Ilsan Paik Hospital, Inje University, Goyang, Korea.

2Division of Pulmonary and Critical Care Medicine, Asan Medical Center, University of ulsan College of Medicine, Seoul, Korea.

3Asan Institute for Life Sciences, Seoul, Korea.

Address for correspondence: Younsuck Koh, M.D. Division fo Pulmonary & Critical Care Medicine, Asan Medical Center, University of Ulsan College of Medicine, 388-1 Pungnap Dong, Songpa Ku, Seoul, 138-736, Korea. Fax: 02-3010-3134, Phone: 02-3010-6968, yskoh@amc.seoul.kr

Received 11 December 2006       Accepted 9 February 2007

Copyright © 2007 The Korean Academy of Tuberculosis and Respiratory Diseases

Abstract

Background

Oxidative stress may play an important role in the pathogenesis of endotoxin-induced acute lung injury (ALI). This study evaluated the therapeutic effect of α-lipoic acid, a nonenzymatic antioxidant, in a rat model of lipopolysaccharide (LPS) induced ALI.

Materials and Methods

ALI was induced in Sprague-Dawley rats by instilling LPS (E.coli, 3mg/Kg) into the trachea. The rats were classified into the control, control+α-lipoic acid, LPS, and LPS+α-lipoic acid groups.The lung lavage neutrophil count, cytokine-induced neutrophil chemoattractant (CINC), lung myeloperoxidase (MPO), and cytokine concentrations (TNF-α, IL-1β, IL-6 and IL-10) were measured at 2 h and 6 h after LPS administration.

Results

The total cell and neutrophil counts of the LPS+α-lipoic acid groups were significantly lower than the LPS groups. The protein concentration in the BAL fluid was similar in the LPS groups and LPS+α-lipoic acid groups. The TNF-α, IL-1β, and IL-6 concentrations in the BAL fluid were not decreased by the α-lipoic acid treatment in the LPS treated rats.

Conclusions

Although α-lipoic acid decreased the level of LPS-induced neutrophil infiltration into the lung, it could not attenuate the LPS-induced ALI at the dose administered in this study.

Keywords: α-lipoic acid, oxidative stress, acute lung injury, antioxidant

Figures and Tables

Figure 1
The effect of α-lipoic acid on the level of CINC of BAL fluid in rats treated with LPS injection (L: LPS group, LA: LPS+α-lipoic acid group) (*p<0.05)
trd-62-105-g001
Figure 2
The effect of α-lipoic acid on the level of MIP of BAL fluid in rats treated with LPS injection (L: LPS, LA: LPS+α-lipoic acid) (*p<0.05)
trd-62-105-g002
Figure 3
The effect of α-lipoic acid on the level of cytokines of BAL fluid in rats treated with LPS (C: control, A: α-lipoic, L: LPS, LA: LPS+α-lipoic acid) (*p<0.05)
trd-62-105-g003
Table 1
Total cell count and neutrophil count in BAL fluid at 2 hour after LPS intratracheal (IT) injection
trd-62-105-i001

*p value; versus Control group, †p value; versus LPS group

Table 2
Total cell count and neutrophil count in BAL fluid at 6 hour after LPS injection
trd-62-105-i002

*p value; versus Control group, †p value; versus LPS group

Table 3
Lung myeloperoxidase (MPO) after LPS injection
trd-62-105-i003

*p value; versus Control group, †p value; versus LPS group

Table 4
Protein concentration in BAL fluid after LPS injection
trd-62-105-i004

*p value; versus Control group, †p value; versus LPS group

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