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Abstract
Purpose
To investigate the effectiveness of the pediatric blood culture bottle for vitreous sample culture in endophthalmitis patients.
Methods
All consecutive cases with clinically suspected endophthalmitis treated and cultured in our institution between January 2009 and June 2013 were included in the study. Vitreous samples were obtained by vitreous needle aspiration (tap), anterior chamber aspiration, or mechanized vitreous biopsy (vitrectomy). The samples obtained using the conventional method until August 2011 were classified as group I. Since August 2011, the BacT/Alert PF pediatric blood culture bottle (bioMérieux, Marcy l’Etoile, France) was used for culture in group II. We investigated age, gender, biopsy method, cause of infection, use of anti-biotics, bacterial culture, and culture positive rate.
Results
Thirty-three cases were included in group I and 17 cases in group II. There was no significant difference in age, gender, sampling technique, cause of infection, and use of antibiotics between the 2 groups. The culture positive rate in group II (60.7%) was significantly higher than group I (33.3%, p = 0.032). In group II, Enterococcus feacalis was the most common pathogen (8 eyes). In group I, Streptococcus pneumoniae and Pseudomonas aeruginosa were confirmed in 3 cases.
Conclusions
The pediatric blood culture bottle can be used successfully in the examination of clinically suspected endophthalmitis. The method showed higher culture positive rate compared with the conventional method. This technique is simple and maintain-ing a supply of fresh agar media is not necessary.
References
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Figure 1.
Conventional culture media. Blood agar plate (A), chocolate agar (B), thioglycollate broth (C), sabouraud dex-trose agar (D), and slide glasses for Gram stain and KOH test (E).
Figure 2.
BacT/Alert® PF (bioMérieux, Marcy l’Etoile, France). A widely used broth culture medium for blood and other body fluids in pediatric practice. It is suitable for bacteria as well as fungi.
Figure 3.
Culture positive rate between two groups. Graphs shows that culture positive rate of pediatric blood culture bot-tle group is higher than that of conventional method group ( p = 0.032).
Table 1.
Baseline characteristics of this study between the conventional method group and pediatric blood culture bottle method group
| Conventional method | Pediatric blood culture bottle | p-value | |
|---|---|---|---|
| Period | January 2009 to August 2011 | August 2011 to June 2013 | |
| Number (n, %) | 33 (54.1) | 28 (45.9) | |
| Sex (male/female) | 13/20 | 11/17 | 0.601* |
| Age (years) | 65.6 ± 14.1 | 70.4 ± 11.0 | 0.153† |
| Sampling technique | 0.082∗ | ||
| Vitrectomy (n, %) | 17 (51.5) | 22 (78.6) | |
| Vitreous and anterior chamber tapping (n, %) | 16 (48.5) | 6 (21.4) | |
| Cause of endophthalmitis | 0.317‡ | ||
| Postoperative (n, %) | 23 (69.7) | 17 (60.7) | |
| Trauma (n, %) | 6 (18.2) | 2 (7.1) | |
| Endogenous (n, %) | 4 (12.1) | 9 (32.1) |
Table 2.
Frequency of organism according to the result of culture
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