Identification and partial purification of antibacterial compounds against Streptococcus mutans from Galla Rhois

Ae-Ri Shin; Seung-Ho Ohk; Choong-Ho Choi; Suk-Jin Hong

doi:10.11149/jkaoh.2016.40.1.3

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Shin, Ohk, Choi, and Hong: Identification and partial purification of antibacterial compounds against Streptococcus mutans from Galla Rhois

Original Article

J Korean Acad Oral Health 2016;40(1):3-8.

Published online: 12 March 2016

DOI: https://doi.org/10.11149/jkaoh.2016.40.1.3

Identification and partial purification of antibacterial compounds against Streptococcus mutans from Galla Rhois

Ae-Ri Shin¹, Seung-Ho Ohk^2,³, Choong-Ho Choi^1,³, Suk-Jin Hong^1,³

¹Department of Preventive & Public Health Dentistry, Chonnam National University School of Dentistry, Gwangju, Korea

²Department of Oral Microbiology, Chonnam National University School of Dentistry, Gwangju, Korea

³Dental Science Research Institute, Chonnam National University School of Dentistry, Gwangju, Korea

Corresponding Author: Suk-Jin Hong Department of Preventive & Public Health Dentistry, Chonnam National University School of Dentistry, 33 Yongbong-ro, Buk-gu, Gwangju 61186, Korea Tel: +82-2-62-530-5835 Fax: +82-2-62-225-9618 E-mail: sjhong@chonnam.ac.kr

Received 10 September 2015 Revised 14 March 2016 Accepted 21 March 2016

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objectives

This study aims to identify and partially purify antibacterial compounds against Streptococcus mutans from Galla Rhois extract.

Methods

Galla Rhois was extracted with n-hexane or ethanol and concentrated in a rotary evaporator. The antibacterial effect of the Galla Rhois extract against S. mutans was determined by the paper disc-diffusion method with n-hexane, ethanol, methanol, ethyl acetate, dimethyl sulfoxide (DMSO), acetone, and distilled water as the solvents. The active compounds were purified by partition chromatography, thin-layer chromatography (TLC), and high-performance liquid chromatography (HPLC).

Results

The antibacterial effect of the n-hexane extract was more effective against S. mutans than the ethanol extract (P<0.05). The antibacterial component of Galla Rhois was partially purified using partition chromatography and HPLC, and the antibacterial activity was confirmed.

Conclusions

The partially purified component of Galla Rhois showed strong antibacterial effect against S. mutans. These results confirm that the antibacterial compounds of Galla Rhois can be used for the prevention of dental caries.

Keywords: Antibacterial compounds, Column chromatography, Galla Rhois, Partition chromatography, Streptococcus mutans, Thin-layer chromatography

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Fig. 1.

Clear zone diameter of S. mutans in extractant solution of Galla Rhois extract.

Fig. 2.

Growth inhibitory activity of n-hexan extract from Galla Rhois aginst S. mutans by paper disc diffusion method after partition chromatography (a: n-hexan, b: Galla Rhois n-hexan extract, c: n-hexan fraction of Galla Rhois n-hexan extract, d: ethanol fraction of Galla Rhois n-hexan extract).

Fig. 3.

TLC pattern of the n-hexan extract from Galla Rhois.

Fig. 4.

Growth inhibitory activity of n-hexan extract from Galla Rhois aginst S. mutans by paper disc diffusion method after TLC (a: fraction S1, b: fraction S2, c: n-hexan, d: fraction S3, e: fraction S4, f: n-hexan).

Fig. 5.

Chromatogram of n-hexan extract of Galla Rhois by Column chromatography.

Fig. 6.

Growth inhibitory activity of n-hexan extract from Galla Rhois aginst S. mutans by paper disc diffusion method after Column chromatography (a: n-hexan, b: fr. 22, c: n-hexan, d: fr. 23, e: n-hexan, f: fr. 24).

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