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Woo, Park, and Bishop: Differential Signaling via Tumor Necrosis Factor-Associated Factors (TRAFs) by CD27 and CD40 in Mouse B Cells
Original Article

Immune Network 2004; 4(3): 143-154.

Published online: 30 September 2004

DOI: https://doi.org/10.4110/in.2004.4.3.143

Differential Signaling via Tumor Necrosis Factor-Associated Factors (TRAFs) by CD27 and CD40 in Mouse B Cells

So-Youn Woo1, Hae-Kyung Park1, Gail A. Bishop2, 3, 4

1Department of Microbiology, College of Medicine, Ewha Womans University, Seoul 158-710, Korea.

2Department of Microbiology, The University of Iowa, Iowa City, IA 52242, USA.

3Department of Internal Medicine, The University of Iowa, Iowa City, IA 52242, USA.

4Veterans Affairs Medical Center, Iowa City, IA 52242, USA.

Correspondence to: So-Youn Woo, Department of Microbiology, Ewha Womans University College of Medicine, Mok-6-dong 911-1, Yang-chun-gu, Seoul 158-710, Korea. (Tel) 82-2-2650-5740, (Fax) 82-2-2653-8891, soyounwoo@ewha.ac.kr

Copyright © 2004 The Korean Association of Immunologists

(open-access):

This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

CD27 is recently known as a memory B cell marker and is mainly expressed in activated T cells, some B cell population and NK cells. CD27 is a member of tumor necrosis factor receptor family. Like CD40 molecule, CD27 has (P/S/T/A) X(Q/E)E motif for interacting with TNF receptor-associated factors (TRAFs), and TRAF2 and TRAF5 bindings to CD27 in 293T cells were reported.

Methods

To investigate the CD27 signaling effect in B cells, human CD40 extracellular domain containing mouse CD27 cytoplamic domain construct (hCD40-mCD27) was transfected into mouse B cell line CH12.LX and M12.4.1.

Results

Through the stimulation of hCD40-mCD27 molecule via anti-human CD40 antibody or CD154 ligation, expression of CD11a, CD23, CD54, CD70 and CD80 were increased and secretion of IgM was induced, which were comparable to the effect of CD40 stimulation. TRAF2 and TRAF3 were recruited into lipid-enriched membrane raft and were bound to CD27 in M12.4.1 cells. CD27 stimulation, however, did not increase TRAF2 or TRAF3 degradation.

Conclusion

In contrast to CD40 signaling pathway, TRAF2 and TRAF3 degradation was not observed after CD27 stimulation and it might contribute to prolonged B cell activation through CD27 signaling.

Keywords: B cell, CD40, CD27, TRAF2, TRAF3

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