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Abstract
Introduction
Skeletal homeostasis is normally maintained by the stability between bone formation by osteoblasts and bone resorption by osteoclasts. However, the correlation between the inflammatory reaction and osteoblastic differentiation of cultured osteoprogenitor cells has not been fully investigated. This study examined the effects of inflammatory cytokines on the osteoblastic differentiation of cultured human periosteal-derived cells.
Materials and Methods
Periosteal-derived cells were obtained from the mandibular periosteum and introduced into the cell culture. After passage 3, the periosteal-derived cells were further cultured in an osteogenic induction Dulbecco's modified Eagle's medium (DMEM) medium containing dexamethasone, ascorbic acid, and β-glycerophosphate. In this culture medium, tumor necrosis factor (TNF)-αwith different concentrations (0.1, 1, and 10 ng/mL) or interleukin (IL)-1β with different concentrations (0.01, 0.1, and 1 ng/mL) were added.
Results
Both TNF-αand IL-1βstimulated alkaline phosphatase (ALP) expression in the periosteal-derived cells. TNF-αand IL-1βincreased the level of ALP expression in a dose-dependent manner. Both TNF-αand IL-1β also increased the level of alizarin red S staining in a dose-dependent manner during osteoblastic differentiation of cultured human periosteal-derived cells.
References
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Fig. 1.
ALP expression in the periosteal-derived cells treated with TNF-αat 3 days of culture. A. No treatment of TNF-α. B. Treatment of 0.1 ng/mL TNF-α. C. Treatment of 1 ng/mL TNF-α. D. Treatment of 10 ng/mL TNF-α. (ALP: alkaline phosphatase, TNF: tumor necrosis factor)
Fig. 2.
ALP expression in the periosteal-derived cells treated with IL-1βat 3 days of culture. A. Treatment of 0.01 ng/mL IL-1β. B. Treatment of 0.1 ng/mL IL-1β. C. Treatment of 1 ng/mL IL-1β. (ALP: alkaline phosphatase, IL: interleukin)
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