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Abstract
The antioxidant effect of CoQ10 on N-nitrosodiethylamine (NDEA)-induced oxidative stress was investigated in mice. Food intake and body weight were similar in both CoQ10 and control groups during the 3-week experimental period. NDEA significantly increased the activities of typical marker enzymes of liver function (AST, ALT and ALP) both in control and CoQ10 groups. However, the increase of plasma aminotransferase activity was significantly reduced in the CoQ10 group. Lipid peroxidation in various tissues, such as heart, lung, liver, kidney, spleen and plasma, was significantly increased by NDEA, but this increase was significantly reduced by 100 mg/kg of CoQ10. Superoxide dismutase activity increased significantly upon NDEA-induced oxidative stress in both the control and CoQ10 groups with the effect being less in the CoQ10 group. Catalase activity decreased significantly in both the control and CoQ10 groups treated with NDEA, again with the effect being less in the CoQ10 group. The lesser effect on superoxide dismutase and catalase in the NDEA-treated CoQ10 group is indicative of the protective effect CoQ10. Thus, CoQ10 can offer useful protection against NDEA-induced oxidative stress.
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Fig. 1.
The effect of CoQ10 on NDEA-induced lipid peroxidation in murine tissues. Malondialdehyde (MDA) is an index of lipid peroxidation in oxidative stress induced by 200 mg/kg NDEA. CoQ10 in olive oil (100 mg/kg) was orally administered to the CoQ10 group for 3 weeks, while an equal volume of olive oil was orally administered to the control group. Results are means±SD from 8 mice. ∗significantly different from saline group (p<0.05), #significantly different from NDEA control group (p<0.05).
Fig. 2.
The effect of CoQ10 on superoxide dismutase (SOD) activity in murine tissues treated with 200 mg/kg NDEA. CoQ10 in olive oil (100 mg/kg) was orally administered to the CoQ10 group for 3 weeks, while an equal volume of olive oil was orally administered to the control group. Results are means±SD from 8 mice. ∗significantly different from saline group (p<0.05), #significantly different from NDEA control group (p<0.05).
Fig. 3.
The effect of CoQ10 on catalase activity in murine tissues treated with 200 mg/kg NDEA. CoQ10 in olive oil (100 mg/kg) was orally administered to the CoQ10 group for 3 weeks, while an equal volume of olive oil was orally administered to the control group. Results are means±SD from 8 mice. ∗significantly different from each saline group (p<0.05), #significantly different from NDEA group of control (p<0.05).
Fig. 4.
The effect of CoQ10 on intracellular reactive oxygen species (ROS) production induced by 1 mg/ml silica in RAW 264.7 cells. Results are means±SD from 4 separate experiments.
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