Abstract
One important mechanism of drug resistance in acute leukemia is the
overexpression of the multi-drug resistance (MDR1) gene that encodes a 170-kDa
membrane protein called P-glycoprotein. To estimate the incidence and role of
MDR1 gene expression in patients with acute leukemia, we investigated the
expression of MDR1 by using the RT-PCR method in blast cells from 40 cases of de
novo acute leukemia. We found a high frequency of MDR1 gene expression: 10 out
of 20 with de novo acute myeloid leukemia (AML), 8 out of 17 with de novo acute
lymphoblastic leukemia (ALL), and none of the 3 with de novo acute mixed
leukemia, were MDR1 mRNA-positive. No correlation between cluster designation
(CD) surface markers (CD19, CD7, CD13, CD33, CD34, CD14, HLA-DR) and MDR1 gene
expression in AML was found. The complete remission rate was correlated with
MDR1 gene expression. Among 40 evaluable patients examined, 17% (3 of 18) with
MDR1 mRNA-positive reached complete remission versus 77% (17 of 22) with MDR1
mRNA-negative (p=0.044). These results suggest that MDR1 gene expression can be
used as a prognostic factor and may be helpful in determining chemotherapeutic
protocol for patients with acute leukemia.