Abstract
To investigate whether MCP-1, CINC, RANTES, osteopontin and ICAM-1 mRNA could be
induced in cultured rat mesangial cells by interleukin-1β(IL-1beta), tumor
necrosis factor-α (TNF-α) and lipopolysaccharide (LPS), and whether
MCP-1 and CINC gene expression could be modulated by dexamethasone, Northern
blot assays were performed. IL-1β induced MCP-1, CINC, RANTES and ICAM-1 gene
expression in a time dependent manner. IL-1β-induced MCP-1, CINC and ICAM-1
mRNA amount were maximal at 3 hours exposure around 14.5, 15.7, 2.2 folds
increase and IL-1β-induced RANTES mRNA at 24 hours around 2.0 folds.
TNF-α and LPS also induced MCP-1 and ICAM-1 gene expression. TNF-α also
induced RANTES gene expression but LPS did not. On the other hand, IL-1β,
TNF-α and LPS had little effect on osteopontin gene expression but fetal
calf serum could increase osteopontin mRNA. Dexamethasone suppressed the
IL-1β-induced MCP-1 and CINC mRNA. These results suggest that, through these
gene expressions, mesangial cells are able to communicate directly or indirectly
with macrophages or neutrophils, which may lead to glomerulosclerosis.