Journal List > J Korean Acad Nurs > v.45(3) > 1003075

Seo and Jeong: Effects of Blending Oil of Lavender and Thyme on Oxidative Stress, Immunity, and Skin Condition in Atopic Dermatitis Induced Mice

Abstract

Purpose

The purpose of this study was to evaluate the effects of essential oil on oxidative stress, immunity, and skin condition in atopic dermatitis (AD) induced mice.

Methods

This study was a 3×3 factorial design. Factors were oil type (Lavender, Thyme, and 2:1 mixture of lavender and thyme oil [blending oil]) and treatment period (0 day, 7 days, and 21 days). The samples were 45 mice with AD and randomly assigned to nine groups of five mice per group. The dependent variables such as superoxide radical, IgE, degranulated mast cells, and epidermal thickness were measured. Data were collected from February to April in 2014. Descriptive statistics, One-way ANOVA, Two-way ANOVA, and Tukey's HSD test were performed using the SPSS WIN 20.0 program.

Results

Dependent variables were not statistically significantly different by the three oil types (p>.05). Essential oils such as lavender, thyme, and blending oil were all effective in reducing AD symptoms and especially 2:1 blending oil were most effective. There were statistically significant differences by the three treatment periods in all dependent variables (p<.001). There were statistically significant interactions between oil types and treatment periods in all dependent variables (p<.01). For decreasing superoxide radical, degranulated mast cells, and epidermal thickness, 2:1 mixed oil should be applied for at least 21 days. Otherwise to reduce IgE, 2:1 mixed oil should be used for at least 7 days.

Conclusion

These findings provide bases for developing effective interventions for AD patients to manage their AD symptoms.

Figures and Tables

Figure 1

Measured variable on skin barrier.

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Figure 2

Photographs of skin in atopic dermatitis induced mice (1 to 9 number means the treatment period and oil type).

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Table 1

Comparison of Superoxide Radical, IgE, Degranulated Mast Cell, and Epidermal Thickness by Oil types and Treatment Period (N=45)

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*Tukey's HSD.

Table 2

Interactions between Oil Types and Treatment Periods (N=45)

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Notes

This manuscript is based on a part of the first author's doctoral dissertation from Chonbuk National University.

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