Journal List > J Korean Ophthalmol Soc > v.50(7) > 1008309

Moon, Kim, Kim, Yoo, and Kwak: The Comparison of Macular Thickness Measurements and Repeatabilities Between Time Domain and Spectral Domain OCT

Abstract

Purpose

To compare macular thickness measurements obtained from time domain optical coherence tomography (TD-OCT) and 2 spectral domain (SD) OCTs and to evaluate their repeatability and agreement in normal subjects and diabetic macular edema patients.

Methods

Fifty-four healthy, normal subjects and 26 diabetic macular edema patients were participated in this study. In a randomly selected eye from each subject, two serial macular measurements were obtained from TD-OCT (Stratus OCT) and SD-OCTs (Cirrus HD-OCT, Spectralis HRA+OCT) by an experienced technician in random order. Nine areas of macular thickness map and repeatabilities obtained by the 3 OCTs were compared.

Results

In relative repeatability, SD-OCT showed better results overall compared to TD-OCT. Macular thickness was greatest in the Spectralis HRA+OCT in both normal subjects and diabetic macular edema patients, followed by Cirrus HD-OCT and Stratus OCT. In normal subjects, regardless of the type of comparison between the machines there was a statistically significant difference in all 9 areas.

Conclusions

While the TD-OCT and the 2 SD-OCTs are reliable for macular thickness measurement, SD-OCT has better measurement repeatability compared with TD-OCT. Because macular measurements obtained from the 3 OCT systems cannot be interchanged, an effort should be made to standardize the measurement of each system.

References

1. Drexler W, Fujimoto JG. State-of-the-art retinal optical coherence tomography. Prog Retin Eye Res. 2008; 27:45–88.
crossref
2. van Velthoven ME, Faber DJ, Verbraak FD, et al. Recent developments in optical coherence tomography for imaging the retina. Prog Retin Eye Res. 2007; 26:57–77.
crossref
3. Gupta V, Gupta P, Singh R, et al. Spectral-Domain Cirrus High-Definition Optical Coherence Tomography Is Better than Time-Domain Stratus Optical Coherence Tomography for Evaluation of Macular Pathologic Features in Uveitis. Am J Ophthalmol. 2008; 145:1018–22.
crossref
4. Leung CK, Chan WM, Yung WH, et al. Comparison of macular and peripapillary measurements for the detection of glaucoma: an optical coherence tomography study. Ophthalmology. 2005; 112:391–400.
5. Kanadani FN, Hood DC, Grippo TM, et al. Structural and functional assessment of the macular region in patients with glaucoma. Br J Ophthalmol. 2006; 90:1393–7.
crossref
6. Chen TC, Cense B, Pierce MC, et al. Spectral Domain Optical Coherence Tomotraphy: Ultra-high Speed, Ultra-high Resolution Ophthalmic Imaging. Arch Ophthalmol. 2005; 123:1715–20.
7. Yi K, Chen TC, de Boer JF. Spectral domain optical coherence tomography. Techniques in Ophthalmology. 2006; 4:170–4.
crossref
8. Age-Related Eye Disease Study Group. The Age-Related Eye Disease Study Sevberity Scale for Age Related Macular Degeneration: Areds Report No. 17. Arch Ophthalmol. 2005; 123:1484–98.
9. Bland JM, Altman DG. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet. 1986; 1:307–10.
crossref
10. Bland JM, Altman DG. Comparing methods of measurement: why plotting difference against standard method is misleading. Lancet. 1995; 346:1085–7.
crossref
11. Leung CK, Cheung CY, Weinreb RN, et al. Comparison of Macular Thickness Measurements between Time Domain and Spectral Domain Optical Coherence Tomography. Invest Ophthalmol Vis Sci. 2008; 49:4893–7.
crossref
12. Kakinoki M, Sawada O, Sawada T, et al. Comparison of macular thickness between Cirrus HD-OCT and Stratus OCT. Ophthalmic Surg Lasers Imaging. 2009; 40:135–40.
crossref
13. Forooghian F, Cukras C, Meyerle CB, et al. Evaluation of Time Domain and Spectral Domain Optical Coherence Tomography in the Measurement of Diabetic Macular Edema. Invest Ophthalmol Vis Sci. 2008; 49:4290–6.
crossref
14. Massin P, Vicaut E, Haouchine B, et al. Reproducibility of retinal mapping using optical coherence tomography. Arch Ophthalmol. 2001; 119:1135–42.
crossref
15. Polito A, Del Borrello M, Isola M, et al. Repeatability and reproducibility of fast macular thickness mapping with stratus optical coherence tomography. Arch Ophthalmol. 2005; 123:1330–7.
crossref
16. Gurses-Ozden R, Teng C, Vessani R, et al. Macular and retinal nerve fiber layer thickness measurement reproducibility using optical coherence tomography (OCT-3). J Glaucoma. 2004; 13:238–44.
17. Danis RP, Fisher MR, Lambert E, et al. Results and repeatability of retinal thickness measurements from certification submissions. Arch Ophthalmol. 2008; 126:45–50.
crossref
18. Chan A, Duker JS. A standardized method for reporting changes in macular thickening using optical coherence tomography. Arch Ophthalmol. 2005; 123:939–43.
crossref

Figure 1.
AREDS grid at right used for reporting retinal thickness (F=fovea; S1=superior inner;, N1=nasal inner; I1=inferior inner; T1=temporal inner; S2=superior outer; N2=nasal outer; I2: inferior outer; T2=temporal outer).
jkos-50-1050f1.tif
Figure 2.
Comparison of macular thickness among the three OCTs in nine areas (A=normal; B=diabetic macular edema) (F=fovea; S1=superior inner; N1= nasal inner; I1=inferior inner; T1=temporal inner; S2=superior outer; N2=nasal outer; I2=inferior outer; T2=temporal outer).
jkos-50-1050f2.tif
Figure 3.
Comparison of the three OCT's relative repeatability (%) (A=normal; B=diabetic macular edema) (F=fovea; S1=superior inner; N1=nasal inner; I1= inferior inner; T1=temporal inner; S2=superior outer; N2=nasal outer; I2=inferior outer; T2=temporal outer).
jkos-50-1050f3.tif
Figure 4.
Bland-Altman plots of the three OCTs. The mean difference is represented by the blue solid line and the 95% confidence limits, by the dotted lines (A=normal; B=diabetic macular edema).
jkos-50-1050f4.tif
Figure 5.
Illustration of how time domain and spectral domain OCT samples the retina differently (Right= SD-OCT; Left=TD-OCT).
jkos-50-1050f5.tif
Figure 6.
Tomographies of the macula taken by the three OCTs with reference lines. All three anterior lines are similar to the ILM, but each posterior line is situated differently.
jkos-50-1050f6.tif
Table 1.!
Mean values and reliabilities of macular thickness parameter in Stratus OCT
A Macular thickness (um) Difference (um) COR (um) RR (%)
Fovea 193.33±18.19 −1.04±5.03 ±9.86 5.1
Superior 1 270.13±13.82 −0.59±3.97 ±7.79 2.88
Nasal 1 270.66±15.17 −0.13±3.44 ±6.75 2.49
Inferior 1 261.90±15.62 1.35±5.98 ±11.73 4.48
Temporal 1 255.25±13.25 −1.43±5.46 ±10.69 4.19
Superior 2 224.49±15.64 0.65±3.34 ±6.54 2.91
Nasal 2 248.03±15.57 −0.50±3.48 ±6.83 2.75
Inferior 2 219.56±15.76 −1.26±4.97 ±9.74 4.44
Temporal 2 210.15±14.07 −1.15±3.73 ±7.32 3.48
B Macular thickness (um) Difference (um) COR (um) RR (%)
Fovea 329.67±148.75 4.42±23.27 ±45.61 13.84
Superior 1 353.31±91.38 1.08±16.56 ±32.45 9.18
Nasal 1 342.46±93.40 3.62±25.07 ±49.13 14.35
Inferior 1 337.63±99.98 1.73±9.48 ±18.58 5.5
Temporal 1 344.15±123.26 4.15±17.23 ±33.77 9.81
Superior 2 305.15±69.31 −0.62±14.11 ±27.66 9.06
Nasal 2 295.35±49.71 1.46±8.40 ±16.46 5.57
Inferior 2 274.19±46.32 1.69±11.77 ±23.08 8.42
Temporal 2 296.23±78.11 3.46±20.77 ±40.72 13.74

A=normal; B=diabetic macular edema.

Table 2.!
Mean values and reliabilities of macular thickness parameter in Cirrus HD-OCT
A Macular thickness (um) Difference (um) COR (um) RR (%)
Fovea 253.01±20.55 −0.39±5.86 ±11.49 4.54
Superior 1 321.56±15.40 −0.67±4.70 ±9.21 2.86
Nasal 1 324.54±16.20 −0.41±3.45 ±6.76 2.08
Inferior 1 315.45±16.86 −0.80±4.14 ±8.11 2.57
Temporal 1 307.16±13.85 −0.54±4.55 ±8.91 2.9
Superior 2 273.01±14.85 0.06±4.02 ±7.88 2.89
Nasal 2 295.36±17.21 0.31±2.60 ±5.09 1.72
Inferior 2 263.87±19.03 −1.30±3.89 ±7.63 2.89
Temporal 2 256.81±14.70 −0.26±4.90 ±9.61 3.74
B Macular thickness (um) Difference (um) COR (um) RR (%)
Fovea 379.46±143.07 1.08±11.67 ±22.88 6.03
Superior 1 401.08±87.04 −1.77±5.24 ±10.27 2.56
Nasal 1 385.46±92.59 0.85±5.59 ±10.96 2.84
Inferior 1 380.33±106.23 0.42±8.22 ±16.11 4.24
Temporal 1 393.90±118.90 0.58±8.00 ±15.67 3.98
Superior 2 337.42±58.54 0.85±12.12 ±23.76 7.04
Nasal 2 338.29±52.05 0.12±5.01 ±9.83 2.91
Inferior 2 312.17±49.30 −0.19±5.17 ±10.13 3.25
Temporal 2 331.31±70.06 −0.69±14.45 ±28.32 8.55

A=normal; B=diabetic macular edema.

Table 3.!
Mean values and reliabilities of macular thickness parameter in Spectralis HRA+OCT
A Macular thickness (um) Difference (um) COR (um) RR (%)
Fovea 268.94±18.88 0.11±4.50 ±8.82 3.28
Superior 1 344.89±14.32 −1.15±4.86 ±9.52 2.76
Nasal 1 346.19±15.36 −0.61±2.48 ±4.87 1.41
Inferior 1 339.81±14.67 −0.07±2.90 ±5.68 1.67
Temporal 1 330.95±14.40 −0.09±2.47 ±4.85 1.47
Superior 2 296.20±15.08 0.22±4.14 ±8.11 2.74
Nasal 2 314.36±14.81 −0.24±2.96 ±5.81 1.85
Inferior 2 285.29±16.42 −0.83±3.27 ±6.41 2.25
Temporal 2 282.13±16.06 −0.81±3.55 ±6.96 2.47
B Macular thickness (um) Difference (um) COR (um) RR (%)
Fovea 396.81±147.34 −2.00±9.02 ±17.68 4.46
Superior 1 422.42±94.92 −0.85±5.23 ±10.25 2.43
Nasal 1 401.08±89.37 0.54±8.61 ±16.87 4.21
Inferior 1 398.56±99.83 −3.35±7.63 ±14.96 3.75
Temporal 1 416.67±120.95 −2.50±11.49 ±22.53 5.41
Superior 2 372.98±69.02 1.73±10.60 ±20.77 5.57
Nasal 2 352.40±51.71 1.65±7.82 ±15.33 4.35
Inferior 2 334.27±48.16 0.85±24.73 ±48.46 14.5
Temporal 2 359.73±74.40 0.31±8.47 ±16.61 4.62

A=normal; B=diabetic macular edema.

Table 4.
Mean and standard deviation of each OCT's macular thickness measurement difference. In the comparison of each OCT's macular thickness, all 9 areas showed a significant difference of p<0.001 (* paired t-test. all of them (9 areas))
A Spectralis-Stratus
Cirrus-Stratus
Spectralis-Cirrus
Mean SD Mean SD Mean SD
F 75.6111 5.73031 59.6759 6.64757 15.9352 6.04624
S1 74.7593 4.29429 51.4259 6.68525 23.3333 7.47575
N1 75.537 5.90718 53.8796 6.55667 21.6574 7.64472
I1 77.9167 6.07481 53.5556 7.39816 24.3611 8.86397
T1 75.7037 5.28572 51.9074 6.22387 23.7963 5.4561
S2 71.713 6.10799 48.5185 7.09236 23.1944 7.10794
N2 66.3333 7.43335 47.3333 6.76004 19 9.52999
I2 65.7315 16.2012 44.3148 7.63637 21.4167 18.9507
T2 71.9815 6.11984 46.6667 6.3558 25.3148 6.98603
Mean 72.8097 * p<0.001 50.8086 * p<0.001 22.001 * p<0.001
B Spectralis-Stratus
Cirrus-Stratus
Spectralis-Cirrus
Mean SD Mean SD Mean SD
F 67.1346 29.8635 49.7885 48.0687 17.3462 41.9983
S1 69.1154 17.533 47.7692 28.2727 21.3462 24.6007
N1 58.6154 26.4433 43 30.5382 15.6154 38.1692
I1 60.9231 24.5025 42.6923 29.6496 18.2308 25.2461
T1 72.5192 28.0323 49.75 30.6 22.7692 24.5301
S2 67.8269 15.2983 32.2692 63.2274 35.5577 57.2951
N2 57.0577 15.326 42.9423 13.4784 14.1154 18.4772
I2 60.0769 21.3423 37.9808 23.3784 22.0962 22.6186
T2 63.5 24.4847 35.0769 27.7992 28.4231 29.4206
Mean 64.0855 42.3632 21.7222

A=normal; B=diabetic macular edema.

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