Various types of cysts that involve root apexes can occur in the jaw. According to the literature, periapical cysts are the most frequent cyst followed by dentigerous cysts and nasopalatine duct cysts¹. Clinical situations that make it challenging to differentiate odontogenic cysts from non-odontogenic cysts and benign tumors include cases with cystic conditions accompanied by secondary infection, impacted teeth, cortical thinning and expansion, and external root resorption^2,3.

Periapical cysts, also known as radicular cysts, are the most common type of jaw cyst, making up about half to two-thirds of all such lesions. On radiographs, continuity of lamina dura to the hyperostotic border is observed. These cysts typically develop from a periapical granuloma, which forms from the remnants of dead dental pulp. Chronic inflammation in this area initially triggers the cell rests of Malassez to proliferate, leading to the formation of the cyst⁴.

Dentigerous cysts, which account for around one-sixth of dental cysts, are a fluid-filled expansion of the dental follicle and are attached to the tooth’s crown at the junction between the enamel and cementum. Radiologically, they do not include the dental root⁴.

Nasopalatine or incisive canal cysts originate from remnants of the nasopalatine ducts that were present during embryonic development. Cyst formation can be caused by local factors such as infection and trauma⁴. When differentiating them from periapical cysts, vitality testing of the teeth involved teeth and continuity of the lamina dura on radiographs from different angles can be employed³. In differential diagnosis with dentigerous cyst, the relationship with the impacted mesiodens should be confirmed³.

Infection is the most common postoperative complication of enucleation and curettage. An oroantral fistula is also a possible complication and may require a buccal advancement flap or a buccal fat pad advancement technique. A pathologic fracture may also occur in the mandible during the late postoperative period until sufficient bone regeneration has occurred⁵.

Intraoperatively, if the lesion appears to be a benign tumor, enucleation and curettage may not be suitable and it could require resection instead⁵. If it is highly suspicious for benign tumor and an apicoectomy is planned, root canal therapy of the adjacent tooth could be necessary. For cysts affecting the apices of anterior teeth, electric pulp testing should be conducted, with nonvital teeth undergoing root canal therapy⁶.

Interestingly, some studies indicate that even when a large cyst is associated with an erupted tooth, vitality can be preserved despite significant bone loss⁶. Infection in cysts may cause a temporary loss of vital response due to increased pressure, potentially leading to root resorption, pulpal ischemia, or necrosis⁶. In cases where a cyst impacts the apical region of a healthy tooth, blood flow to the pulp is maintained through the cyst capsule⁶. Removing the cyst lining can risk necrosis in adjacent teeth; thus, regular follow-up is crucial for monitoring the pulp vitality of teeth preserved without root canal therapy⁶.

Previous studies about similar procedures have indicated that necrosis of adjacent teeth is extremely rare after maxillary sinus elevation (1/221)⁷, and although pulp fibrosis is sometimes observed after posterior segmental osteotomy⁸, it does not affect the prognosis of the teeth as many pulp tissues heal spontaneously, suggesting that root canal therapy should be postponed until absolutely necessary. Additionally, the nerves of adjacent teeth might regenerate post-surgery due to vascular endothelial growth factor and dental pulp stem cells⁶.

Preoperative root canal therapy should be carefully considered by weighing the regenerative potential against the risk of complications, as tooth vitality will be lost. This study aims to identify risk factors for complications in patients undergoing cyst enucleation of the jaw, proposes a clinical model for predicting complications, and, based on this, identifies indications for preoperative root canal therapy of adjacent teeth.

The study included 77 patients, 55 men and 22 women (men:women, 2.5:1), and resulted in 10 discomforts and complications.(Table 2) In this study, cases that did not undergo electric pulp testing existed, so this factor was excluded from analysis. There were no preoperative root canal therapy-related complications such as root fracture.

As complications, the following were observed: 5 cases of surgical site infection, 2 cases of reduced sensation in teeth or skin, 1 case of tooth discoloration, 1 case of a secondary primary lesion at the same site, and 1 case of recurrence. There was 1 case of postoperative root canal therapy due to infection and 1 case of postoperative root canal therapy due to discoloration of the other tooth. Every complication was Clavien–Dindo Grade ≤IIIa⁹. On Fisher’s exact test, there was no significant difference in complication rates in procedures using particulate bone material (P=0.084); however, this was at the borders of the confidence limit (P<0.1).(Table 3)

For analysis of the complication risk of each clinical variable, Fisher’s exact test or linear by linear association was used. Cyst size (P=0.005, odds ratio [OR]=8.034) and procedure (P=0.011, OR=13.45) were significant variables.

On univariable logistic regression, the size and procedure (C: cyst enucleation only, +AB: with apicoectomy, bone graft, +B: with bone graft) were significantly associated with a higher risk of complications.(Table 4) The selected variables were tested after controlling for other variables using multivariable logistic regression. The chi-square test was used for model coefficient total test (P=0.002). The Hosmer–Lemeshow test was used to check the goodness of fit and calibration for logistic regression models (P=0.907). On multicollinearity, tolerance was over 0.7 and the variance inflation factor was under 1.2. Larger size raised the risk of complication by 1.127 (P=0.047). Cyst enucleation only (C) raised complication risk by 17.716 compared to cyst enucleation with extraction (P=0.027). Cyst enucleation with apicoectomy and bone graft (+AB) increased complication risk by 33.939 compared to cyst enucleation with extraction (P=0.031). Cyst enucleation including bone graft (+B) elevated complication risk by 52.751 compared to cyst enucleation with extraction (P=0.009).(Table 5)

A nomogram for predicting complications was developed based on the logistic regression model. All variables were included after categorization.(Fig. 3) Procedure type was deemed to be the most critical factor. The blue points represent the fact that the bigger cyst size, treatment without extraction or apicoectomy, and treatment with bone graft has probability of discomfort by 48%.

We propose a clinical model for predicting complications in patients undergoing cyst enucleation of the jaw to facilitate decision-making in clinical situations where it is difficult to differentiate between odontogenic and non-odontogenic cyst and benign tumors.

Additional procedures such as apicoectomy, extraction, and preoperative root canal therapy lower the risk of complications, with the exception of bone graft. Previous studies suggested that particulate bone grafts may increase the risk of postoperative infection¹⁰, which was consistent with our results. Some researchers have even argued that bone defects left after curettage may not need bone grafting for successful outcomes¹¹. However, grafting large bone defects offers several structural and biological benefits that can accelerate healing and help restore normal function¹². For future implant sites, the bone graft can be beneficial¹¹. In large bone defects and sites prepared for future implants, achieving careful primary closure of the grafted area is essential, with the use of a membrane whenever necessary.

Larger lesions result in more extensive bone defects. A previous study found that critical-size alveolar bone defects (≥20 mm) did not heal completely within one year¹¹. The median percentage reduction in defect volume was 98% for the bone graft group compared to 73% for the control group (P=0.001), with evidence of soft tissue invagination in the control group¹¹. This invagination can lead to trapping of food debris postoperatively. Proprioception and vitality, which help protect the wound during healing, combined with the inaccessibility of the surgical site, makes it very difficult for patients to clean the area conventionally¹³. Enucleating a large cyst can also increase the risk of damaging nerves connected to the dental pulp. In such cases, marsupialization is an option if tooth vitality might be compromised, although neoplastic changes in the cystic lining cannot be confirmed. Therefore, enucleation remains a viable option, with close postoperative follow-up being essential.

As another factor related to pathology, periapical abscesses and granulomas tend to carry the highest risk of complications. If left untreated, these periapical lesions can lead to severe complications due to the spread of infection. Potential complications include osteomyelitis, cellulitis, bacteremia, and the formation of a fistulous tract that opens into the oral cavity or skin. In severe cases, cavernous sinus thrombosis may also occur¹⁴. Surgical site infections caused by microorganisms are more frequent in cases involving acute abscesses and are predominantly due to anaerobic bacteria¹⁵. Secondary acute apical periodontitis is acute exacerbation of an existing chronic apical periodontitis lesion¹⁶. This condition can manifest as a secondary apical abscess when bacteria migrate from the root canal to infect the periapical tissue, although other local or systemic changes may also trigger acute inflammatory responses¹⁷. Surgical intervention may be a contributing factor to acute exacerbation in periapical granuloma. In contrast, periapical cysts show a lower incidence of complications, possibly due to their more chronic nature¹⁶ and the potential protective role of host immunity.

There has been unpublished report that preoperative root canal treatment reduces postoperative complications. However, in this study, we did not observe that association due to the small sample size. Since postoperative EPT was positive even in some patients who had a negative EPT preoperatively, it may be possible to decide on root canal treatment for teeth adjacent to the cyst either preoperatively or postoperatively with EPT reevaluation after providing information on the risks and benefits to the patient. As another approach, EPT results can serve as a reference for this decision especially in anterior teeth. In this study, a treatment plan based on EPT showed a lower incidence of complications. Additionally, preoperative radiographic diagnosis may indicate the need for preoperative root canal treatment and apicoectomy if invasive lesions are suspected.

Age was not a factor influencing complications. Considering wound healing delay in elderly patents, this might be due to fast growth patterns of cysts in younger patients¹⁸. Women gender tended to be related to higher risk. In postmenopausal women, decreased immunity and healing ability¹⁹ may have an effect, or the patient’s deppression could be a factor. Staff have a tendency of a risk factor unexpectedly. In addition to larger lesions, other factors such as procedure complexity, unplanned procedures (which carry higher surgical risks), patient expectations, and the involvement of more perioperative staff could affect outcomes²⁰, and long-term follow-up may also have influenced the observed incidence.

This study has several limitations. First, the sample size of patients was small as involved adjacent tooth was needed adequate for inclusion criteria. Furthermore, since this was a single-center study, the findings may not be widely applicable, highlighting the need for multicenter studies to confirm these results across different populations and settings. Second, as a retrospective study, our research is subject to inherent biases and limitations in data collection and analysis. Future research should focus on prospective studies to provide more reliable data and a better understanding of the factors influencing complications.

Within the limitations of our study, additional procedures reduced the risk of complications resulting from jaw cyst enucleation, except for bone grafts. Large cyst size was associated with a higher risk of complications. It is important to consider the possibility of a second procedure and take thorough precautions to prevent infections when performing bone grafts. Patients should be informed of these risks in advance, scheduled for regular follow-up, and provided additional treatment when necessary.