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Shin, Suh, and Suh: Effect of Cadmium and Zinc on IL-6 Secretion in a Human Monocytic Cell Line, THP-1
Original Article

Korean Journal of Occupational and Environmental Medicine 1999; 11(3): 332-339.

Published online: 31 January 2019

DOI: https://doi.org/10.35371/kjoem.1999.11.3.332

Effect of Cadmium and Zinc on IL-6 Secretion in a Human Monocytic Cell Line, THP-1

Dong Hoon Shin, Suk Kwon Suh, Seong IL Suh

Department of Preventive Medicine and Public Health, School of Medicine, Keimyung University, Korea.

Department of Microbiology, School of Medicine, Keimyung University, Korea.

Copyright © 1999 The Korean Society of Occupational and Environmental Medicine

Abstract

OBJECTIVES

This study was performed to investigate effects of cadmium and zinc on IL-6 secretion using human monocyte in a culture system.

METHODS

We have used frypan blue dye exclusion methods to examine the effect of cytotoxicity of CdCl2, ZnCl2 in THP-1 cells. IL-6 was measured by the ELIBA method in the cell culture supernatants and the expression of IL-6 mRNA was examined by reverse transcription-polymerase chain reaction.

RESULTS

IL-6 production by THP-1 cells were decreased at 0.1 mM cadmium concentration(p < 0.05) and increased as zinc concentration increased. Zinc prevented cadmiuminduced suppression of IL-6. and the addition of a chelating agent, EDTA, restores IL-6 secretion. The expression of IL-6 mRNA decreased at 0.08 mM and 0.1 mM cadmium concentrations.

CONCLUSION

This results suggests that cadmium depressed production and gene expression of IL-6 in stimulates human monocytes, and zinc prevented cadmium-induced suppression of IL-16.

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