Journal List > Korean J Gynecol Oncol Colposc > v.15(1) > 1123574

TOOLS
Shon, Cho, Song, Kwon, Kim, Kang, and Cha: Increased Expression of GADD34 in Ovarian Carcinomas
Original Article

Korean Journal of Gynecologic Oncology and Colposcopy 2004; 15(1): 29-34.

Published online: 12 March 2019

DOI: https://doi.org/10.3802/kjgoc.2004.15.1.29

Increased Expression of GADD34 in Ovarian Carcinomas

Won kyung Shon, Chi Heum Cho, Dae Kyu Song, Sang Hoon Kwon, Sook Hyun Kim, Hyoung Ok Kang, Soon Do Cha

Department of Obstetrics and Gynecology, School of Medicine, Keimyung University, Daegu, Korea.

Department of Physiology, School of Medicine, Keimyung University, Daegu, Korea.

Copyright © Korean Society of Gynecologic Oncology and Colposcopy

Abstract

OBJECTIVE

GADD34 was originally described as a growth arrest and DNA damage-inducible gene. Increased expression of GADD34 was subsequently found to correalate with apoptosis, and forced overexpression of the protein leads to apoptosis. In this study we evaluated the expression of GADD34 in ovarian cancers and normal ovarian tissues.

METHODS

Fresh tissues were retrieved from 15 cases with ovarian cancers, 7 normal and 3 benign ovarian tissues. The expression of GADD34 in ovarian cancers compared with normal tissues were examined by RT-PCR, Western-blot analysis and immuno-histochemical analysis.

RESULTS

The overexpression of GADD34 mRNA levels was identified in ovarian cancers compared with normal ovaries by RT-PCR assay. Using Western-blot analysis, the overexpression of GADD34 was observed in ovarian cancer tissues compared with normal ovarian tissues. On histochemical results, the expression of GADD34 were observed in ovarian cancer tissues.

CONCLUSION

Increased expression of GADD34 in the ovarian carcinoma compared to normal ovarian tissues may play a part of role in pathogenesis of ovarian cancer.

Keywords: Ovarian cancer, GADD34

TOOLS
Similar articles