Measles Viral Infection in PD-1 Gene Knockout Mice

Jin Kyong Chun; Kyu Yeun Kim; Ji Ae Hur; Dong Won Kang; Ki Hwan Kim; Dong Soo Kim

doi:10.14776/kjpid.2013.20.3.123

Journal List > Korean J Pediatr Infect Dis > v.20(3) > 1096036

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Chun, Kim, Hur, Kang, Kim, and Kim: Measles Viral Infection in PD-1 Gene Knockout Mice

Original Article

Korean Journal of Pediatric Infectious Diseases 2013; 20(3): 123-130.

Published online: 26 December 2013

DOI: https://doi.org/10.14776/kjpid.2013.20.3.123

Measles Viral Infection in PD-1 Gene Knockout Mice

Jin Kyong Chun, M.D., Kyu Yeun Kim, M.D.^*, Ji Ae Hur, M.D.^*, Dong Won Kang, M.D.^†, Ki Hwan Kim, M.D.^*, Dong Soo Kim, M.D., Ph.D.^*

Department of Pediatrics, Yonsei University College of Medicine, Wonju, Korea.

^*Department of Pediatrics, Yonsei University College of Medicine, Korea.

^†Department of Pharmacology, Kwandong University College of Medicine, Korea.

Corresponding author (dskim6634@yuhs.ac)

Received 19 August 2013 Revised 8 October 2013 Accepted 13 October 2013

Abstract

Purpose

Subacute sclerosing panencephalitis (SSPE) is a neurodegerative disease due to persistent measles virus infection. We investigated the role of programmed death-1 (PD-1) molecule which is related with chronic viral infection in developing SSPE in mouse.

Methods

We adopt the PD-1^-/-, PD-1^-/+, and wild type BALB/c 3 week old mice to make an animal model of SSPE by injecting measles virus (SSPE strain) intraventricularly. Three months after infusion of virus, the mice were sacrificed and examined if the typical pathologic lesions had been progressed. The sera were collected from each group of mice and the serum level of IL-21 was measured with ELISA kit.

Results

The necrotic lesions on white matter and gliosis were found in focal areas in wild type BALB/c. The extent of lesion was smaller in heterotype BALB/c. Scanty lesions were found in PD-1^-/- mice. The sera level of IL-21 was not elevated in all three groups.

Conclusion

Our data suggest that the PD-1 molecule may play a role in persistent viral infection.

Keywords: Subacute sclerosing panencephalitis, Measles, Programmed death-1

Figures and Tables

Fig. 1

Changes of brain parenchyma by SSPE virus in 3 month-old wild type BALB/c. (A) No change was shown in wild mice which was not injected with virus (×200, Nissl staining). (B) Inflammatory changes were observed in the same age of wild mice which was injected with SSPE virus (×400, Nissl staining).

Fig. 2

Comparison of lesions according to PD-1 status. (A) Wild type mouse (×200, Nissl staining) (B) Hetero type mouse (×200, Nissl staining). The inflammation in the myelin portion is more serious in wild type mouse than in heterotype. (C) Wild type mouse (×200, GFAP staining). (D) KO mouse (×200, GFAP staining). The astrocytes are proliferated more in wild type mouse than in KO mouse.

Fig. 3

Comparison of lesions among groups 3 months after SSPE virus was injected into ventricle. In wild type mouse, the necrotic changes and fibrosis are more prominent compared with PD-1 heterogroup or PD-1 KO group.

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