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Lee, An, Kim, Cho, Kim, Jang, Kwak, Lee, Lee, Lee, and Shin: Anti-Inflammatory Effect of Violapyrones B and C from a Marine-derived Streptomyces sp.
Original Article

Natural Product Sciences 2015;21(4):251-254.

Published online: 17 January 2015

DOI: https://doi.org/10.20307/nps.2015.21.4.251

Anti-Inflammatory Effect of Violapyrones B and C from a Marine-derived Streptomyces sp.

Hwa-Sun Lee1, Bong-Jeun An2, Hyeon Jeong Kim2, 3, Yong Hun Cho2, Dong In Kim2, Jae Yoon Jang2, Jae Hoon Kwak2, Hyi-Seung Lee1, Yeon-Ju Lee1, Jong Seok Lee1, Hee Jae Shin1, 4, ∗

1 Marine Natural Products Chemistry Laboratory, Korea Institute of Ocean Science and Technology, 787 Haeanro, Ansan 426-744, Korea

2 Department of Cosmeceutical Science, Dague Haany University, 285-10 Eobongji-gil, Gyeoungsan 712-220, Korea

3 Institute of technology, Herbnoori.Co., Ltd. Daegu, 702-852, Korea

4 Department of Marine Biotechnology, University of Science and Technology, 217 Gajungro, Daejeon 305-350, Korea

∗Author for correspondence Hee Jae Shin, Marine Natural Products Chemistry Laboratory, Korea Institute of Ocean Science and Technology, 787 Haeanro, Ansan 426-744, Korea Tel: +82-31-400-6172; E-mail: shinhj@kiost.ac

      Revised 30 July 201522 September 2015       Accepted 30 September 2015

Copyright © 2015 The Korean Society of Pharmacognosy

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Recently, we reported violapyrones B, C, H and I, unusual 3, 4, 6-trisubstituted α-pyrone derivatives, from the culture broth of the marine Streptomyces sp. 112CH148. In previous studies, violapyrones have been shown to have antibacterial and antitumor activities. However, the anti-inflammatory effect of violapyrones has not been reported yet. As part of our ongoing study for the discovery of bioactive metabolites from marine microorganisms, we found that violapyrones also have anti-inflammatory activity. In this study, we investigated the effect of violapyrones on LPS-induced inflammatory responses in vitro. Violapyrones B and C did not affect the viability of RAW 264.7 cells at concentrations up to 25 μM. However, violapyrones B and C inhibited the production of NO compared to the LPS-induced control. In addition, violapyrones B and C down-regulated the expression of iNOS protein in LPS-stimulated RAW 264.7 cells. To the best of our knowledge, this is the first report on the anti-inflammatory activity of violapyrones B and C.

Keywords: Violapyrones, α-Pyrones, Streptomyces sp., Anti-inflammatory

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Fig. 1.
The structures of violapyrones B and C.
nps-21-251f1.tif
Fig. 2.
Effect of violapyrones B and C on the cell viablility of RAW 264.7 cells.
nps-21-251f2.tif
Fig. 3.
Effect of violapyrones B and C on the production of nitric oxide (NO) in LPS-induced macrophage RAW 264.7 cells.
nps-21-251f3.tif
Fig. 4.
Effect of violapyrones B and C on the expression levels of iNOS and COX-2 proteins in LPS-stimulated macrophage RAW 264.7 cells.
nps-21-251f4.tif
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