A confocal microscopic study on dentinal infiltration of one-bottle adhesive systems and self-etching priming system bonded to class V cavities

Hyung-Su Kim; Sung-Ho Park

doi:10.5395/JKACD.2002.27.3.257

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Kim and Park: A confocal microscopic study on dentinal infiltration of one-bottle adhesive systems and self-etching priming system bonded to class V cavities

Original Article

Journal of Korean Academy of Conservative Dentistry

Journal of Korean Academy of Conservative Dentistry 2002; 27(3): 257-269.

Published online: 31 May 2002

DOI: https://doi.org/10.5395/JKACD.2002.27.3.257

A confocal microscopic study on dentinal infiltration of one-bottle adhesive systems and self-etching priming system bonded to class V cavities

Hyung-Su Kim, Sung-Ho Park

Department of Dentistry, The Graduate School of Dentistry, Yonsei University, Korea.

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective

The purpose of this study was to evaluate the resin infiltration into dentin of one-bottle adhesive systems and self-etching primer bonded to Class V cavities using confocal laser scanning microscope(CLSM).

Material and Methods

Forty Class V cavities were prepared from freshly extracted caries-free human teeth. These teeth were divided into two groups based on the presence of cervical abrasion: Group I, cervical abrasion; Group II, wedge-shaped cavity preparation. Resin-dentin interfaces were produced with two one-bottle dentin bonding systems-ONE COAT BOND(OCB; Coltene®) and Syntac®Srint™(SS; VIVADENT)-, one self-etching priming system-CLEARFIL™ SE BOND(SB; KURARAY)- and one multi-step dentin bonding system-Scotchbond™Multi-Purpose(SBMP, 3M Dental Products)-as control according to manufacturers'instructions. Cavities were restored with Spectrum®(Dentsply). Specimens were immersed in saline for 24 hours and sectioned longitudinally with a low-speed diamond disc. The resin-dentin interfaces were microscopically observed using CLSM. The quality of resin-infiltrated dentin layers were evaluated by five dentists using 0-4 scale.

Results

Confocal laser scanning microscopal investigations using primer labeled with rhodamine B showed that the penetration of the primer occurred along the cavity margins.

Statistical analysis using one-way ANOVA followed by Duncan's Multiple Range test revealed that the primer penetration of the group 2(wedge-shaped cavity preparation) was more effective than group 1(cervical abrasion) and that of the gingival interfaces was more effective than the occlusal interfaces. In the one-bottle dentin bonding systems, the resin penetration score of OCB was compatible to SBMP, but those of SS and self-etching priming system, SB were lower than SBMP.

Figures and Tables

Fig. 1

Diagram of longitudinally sectioned tooth showing cavity shapes (shadowed) and schematic orientation of dentinal tubules to the class V cavity surfaces.

Fig. 2

Resin penetration scores in occlusal interfaces of class V restorations. Asterisk(*) means statistically significant differences(p<0.05)

Fig. 3

Resin penetration scores in gingival interfaces of class V restorations. Asterisk(*) means statistically significant differences(p<0.05)

Fig. 4

Comparison of resin penetrations of occlusal and gingival interfaces in group 1(cervical abrasion). Asterisk(*) means statistically significant differences (p<0.05)

Fig. 5

Comparison of resin penetrations of occlusal and gingival interfaces in group 2(V-shaped cavity preparaion). Asterisk(*) means statistically significant differences(p<0.05)

Fig. 6.1-6.5

Standard confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in occlusal interfaces of Class V cavity used for scoring the resin tag penetrations. Original magnification ×200

6.1. Score 0., 6.2. Score 1., 6.3. Score 2.,

6.4. Score 3., 6.5., Score 4.

Fig. 7.1-7.5

Standard confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in gingival interfaces of Class V cavity used for scoring the resin tag penetrations. Original magnification ×200

7.1. Score 0., 7.2. Score 1., 7.3. Score 2.,

7.4. Score 3., 7.5., Score 4.

Fig. 8

Confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in gingival interfaces of Class V cavity with Scotchbond multi purpose. magnification × 800 23

Fig. 9.1-9.4

Confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in occlusal interfaces of cervical abrasion. Original magnification ×200

Fig. 10.1-10.4

Confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in occlusal interfaces of Class V cavity. Original magnification ×200

Fig. 11.1-11.4

Confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in gingival interfaces of cervical abrasion. Original magnification ×200

Fig. 12.1-12.4

Confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in gingival interfaces of Class V cavity. Original magnification ×200

Table 1

Names, numbers and characteristics of each groups of experiment

Table 2

Chemical components and instructions for use of the dentin bonding systems used in this study

Table 3

Inter-observer correlations of each groups, materials, and parameters

occlu: resin penetrations in occlusal interfaces.

gingi: resin penetrations in gingival interfaces.

Table 4

Resin penetration scores(Mean±SD) at the occlusal interfaces

Comparison among the groups: one-way ANOVA, p<0.05

Table 5

Resin penetration scores(Mean±SD) at the gingival interfaces

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