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Hong, Lee, Kim, and Kim: Postpartum Transmission as a Major Route of Mother-to-Child Helicobacter felis Infection
Original Article

Lab. Anim. Res. 2010;26(3):319-321.

Published online: 17 September 2010

DOI: https://doi.org/10.5625/lar.2010.26.3.319

Postpartum Transmission as a Major Route of Mother-to-Child Helicobacter felis Infection

Sunhwa Hong1, Hyun-A Lee1, Youngho Kim2, ∗, Okjin Kim1, 3, ∗

1Center for Animal Resources Development, Wonkwang University, Iksan, Korea

2Department of Biochemistry, School of Medicine, Wonkwang University, Iksan, Korea

3Institute of Animal Experiment & Efficacy Evaluation, Wonkwang University, Iksan, Korea

∗Corresponding authors: Youngho Kim, Department of Biochemistry, School of Medicine, Wonkwang University, 344-2 Shinyoung-dong, Iksan, Jeonbuk 570-749, Korea TEL: +82-63-850-6795 FAX: +82-63-850-6795 E-mail: youngkim@wku.ac.kr Okjin Kim, Center for Animal Resources Development, Wonkwang University, 344-2 Shinyoung-dong, Iksan, Jeonbuk 570-749, Korea TEL: +82-63-850-6668 FAX: +82-63-850-7308 E-mail: kimoj@wku.ac.kr

Received 27 August 2010       Revised 11 September 2010       Accepted 14 September 2010

Copyright © 2010 Korean Association for Laboratory Animal Science

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In this study we investigated maternal Helicobacter felis (H. felis) infection status to determine the potential of maternal transmission. Pregnant Beagle dogs were infected experimentally with H. felis. Following the experimental design, the stools of the mother and litters were isolated and assessed for transmission of H. felis at parturition day, 1-week old age and 6-week old age respectively. Polymerase chain reaction (PCR) was used to examine the presence of transmitted H. felis. All litters showed no transmission of H. felis at parturition day. However, they revealed 14.3 % and 100 % at 1-week old age and 6-week old age respectively by PCR. These results suggested that vertical infection during prenatal period or delivery procedure is unlikely as a route of mother-to-child H. felis infection. It might be acquired H. felis through breastfeeding, contaminating saliva and fecal-oral during co-habitat.

Keywords: Helicobacter, H. felis, dog, transmission, route, postpartum

REFERENCES

Baele M.., Van den Bulck K.., Decostere A.., Vandamme P.., Hänninen M.L.., Ducatelle R.., Haesebrouck F.2004. Multiplex PCR assay for differentiation of Helicobacter felis, H. bizzozeronii, and H. salomonis. J. Clin. Microbiol. 42(3):1115–1122.
De Bock M.., Decostere A.., Hellemans A.., Haesebrouck F.., Ducatelle R.2006. Helicobacter felis and Helicobacter bizzozeronii induce gastric parietal cell loss in Mongolian gerbils. Microbes Infect. 8(2):503–510.
crossref
Kim S.H.., Kim O.2004. Application of Consensus Polymerase Chain Reaction for Monitoring of Helicobacter Species. Lab. Anim. Res. 20(4):316–320.
Kim S.K.., Cho S.J.., Kim O.2006. Detection and identification of secreting Helicobacter species from cats. Lab. Anim. Res. 22(3):243–247.
Lee H.A.., Park Y.S.., Kim O.2007. Prevalence of Helicobacter Species in Feces of Dogs. Lab. Anim. Res. 23(3):339–344.
Oh H.G.., Kim Y.., Lee H.A.., Kim O.2009. Detection of Helicobacter heilmannii infection in a cat. Lab. Anim. Res. 25(4):385–391.
Rothenbacher D.., Inceoglu J.., Bode G.1999. Helicobacter pylori among preschool children and their parents: evidence of parent-child transmission. J. Infect. Dis. 179(2):398–402.
Rothenbacher D.., Inceoglu J.., Bode G.2000. Acquisition of Helicobacter pylori infection in a high-risk population occurs within the first 2 years of life. J. Pediat. 136(6):744–748.
crossref
Rothenbacher D.., Winkler M.., Gonser T.2002. Role of infected parents in transmission of Helicobacter pylori to their children. Pediatr. Infect. Dis. J. 21(7):674–679.
crossref
Torres J.., Perez-Perez G.., Goodman K.J.2000. A comprehensive review of the natural history of Helicobacter pylori infection in children. Arch. Med. Res. 31(5):431–469.
crossref

Figure 1.
Result of Helicobacter felis specific polymerase chain reaction with the fecal samples. P, positive control; N, negative control; M1, mother 1; M2, mother 2; L1~L7, litter 1~litter 7. On parturition day, there was no positive reaction in litters, while 6 weeks postpartum, all litters showed positive reaction.
lar-26-319f1.tif
Table 1.
Results of polymerase chain reaction for assessment of transmission of Helicobacter felis
Evaluated time Subject % Detection rate of H. felis (No. of positive/No. of animal)
Delivery Mothers 100 (2/2)
Litters 0 (0/7)
1-week postpartum Mothers 100 (2/2)
Litters 14.3 (1/7)
6-week postpartum Mothers 100 (2/2)
Litters 100 (7/7)
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