Journal List > J Korean Acad Periodontol > v.30(1) > 1049173

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Han, Cho, Lim, Kim, Park, and Kim: Immunohistochemical Study of the Expression of Bone Morphogenetic Protein(BMP-7) Following Regenerative Periodontal Surgery
Original Article

The Journal of the Korean Academy of Periodontology 2000; 30(1): 51-63.

Published online: 31 March 2000

DOI: https://doi.org/10.5051/jkape.2000.30.1.51

Immunohistochemical Study of the Expression of Bone Morphogenetic Protein(BMP-7) Following Regenerative Periodontal Surgery

Kyung-Yoon Han1, Se-Yeol Cho1, Ki-Jung Lim1, Heung-Jung Kim2, Ju-Cheol Park3, Byung-Ock Kim3

1Department of Periodontology, College of Dentistry, Chosun University, Korea.

2Department of Oral Anatomy, College of Dentistry, Chosun University, Korea.

3Department of Oral Histology, College of Dentistry, Chosun University, Korea.

Copyright © 2000 Korean Academy of Periodontology

Abstract

Extracellular matrix component is degraded by enzymes of thematrix metalloproteinases(MMPs). MMPs are produced by both hemopoietic and structural cells. Increased activity of MMP-3 in periodontium is strongly associated with inflammatory periodontal disease.
The purpose of the present study was to estimate the effect of BMP-7 on regeneration of periodontium. The optical density was measured by microwell plate reader at 450 nm.The difference of the optical density and the relative activity ofMMP-3 according to the concentration were statistically analyzed by one way ANOVA.
The results were as follows:
1.Tetracycline-HCl showed the tendency to inhibit the activity of MMP-3 at the concentration lower than 25µg/ml.
2.Doxycycline-HCl inhibited significantly the activity of MMP-3 at the concentration lower than 100µg/ml.
3.Minocycline-HCl inhibited the activity of MMP-3 at the concentration in the range of 10 to 200µg/ml.
Within the limit of the present study, the above results suggested that bone morphogenetic protein-7 may play a important role in development of periodontium.

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