Safflower seeds has been used for the treatment of blood stasis, bone fracture and osteoporosis in traditional Korean medicine. The purpose of this study is to examine effects of the isolated extracts from Safflower seeds on mineralization of periodontal ligament cells and osteoblastic cells. Periodontal ligament cells were primarily obtained from a extracted premolars with non-periodontal diseases. Osteoblastic cells were obtained from calvariae of a fetal rat. Cells were cultured with DMEM at 37℃ with 5% CO₂ in 100% humidity incubator. Safflower seeds were isolated into the H₂O layer and the butanol layer. MTT assay and alkaline phosphatase(ALP) level were examined.

Also the number of bone calcification nodules were evaluated.

The obtained results were as follows:

1. The cellular activity of periodontal ligament cells was significantly increased in 10^-3g/ml, 10^-6g/ml of both H₂O layer and butanol layer of Safflower seeds.

2. The cellular activity of osteoblastic cells was significantly increased in 10^-3g/ml, 10^-6g/ml of H₂O layer of Safflower seeds.

3. ALP level of periodontal ligament cells was significantly increased in 10^-3g/ml of both H₂O layer and butanol layer of Safflower seeds.

4. ALP level of osteoblastic cells was significantly increased in 10^-3g/ml, 10^-6g/ml of H₂O layer and especilly more increaton was showed in 10^-3g/ml of H₂O layer.

5. Calcification nodules of periodontal ligament cells slightly increased in 10^-3g/ml of both H₂O layer and butanol layer of Safflower seeds.

6. Calcification nodules of osteoblastic cells slightly increased in 10^-3g/ml, 10^-6g/ ml of H₂O layer of Safflower seeds.

These results indicate that H₂O layer and butanol layer of the isolated extracts from Safflower seeds has excellent effects on mineralization of periodontal cells and osteoblastic cells.