Journal List > J Korean Acad Periodontol > v.28(1) > 1049005

J Korean Acad Periodontol. 1998 Mar;28(1):133-143. Korean.
Published online March 31, 1998.  https://doi.org/10.5051/jkape.1998.28.1.133
Copyright © 1998 Korean Academy of Periodontology
Effect of glucose and insulin on human gingival fibroblasts and periodontal ligament cells
Hee-Ran Han, Eung-Tea Kim, Hyung-Keun You and Hyung-Shik Shin
Department of Periodontology, College of Dentistry, Wonkwang University, Korea.
Abstract

Diabetes mellitus is a systemic disease with profound effects on oral health and periodontal wound healing. Uncontrolled diabetes adversely affects surgical wound healing and is often associated with abnormal proliferation of fibroblasts. Human gingival fibroblasts and PDL cells were chosen because they are intimately involved in periodontal therapy and are important for the success of surgical procedure such as guided tissue regeneration.

The aim of the present study was to elucidate whether cellular activity and collagen synthesis by glucose pre-treated human gingival fibroblasts and PDL cells are influenced by insulin, and whether healthy cells differ from glucose treated cells.

Cells were cultured with DMEM at 37℃, 5% CO2, 100% humidified incubator. To evaluate the effect of glucose on gingival fibroblasts and periodontal ligament cells, the cells were seeded at a cell density of 1×104 cells/well culture plates and treated with 20 and 50mM of glucose for 5 days. Then MTT assay was carried out. To evaluate the effect of insulin on glucose-pretreated cells, the cells were seeded at a cell density of 1×104 cells/well culture plates and treated with 20 and 50mM of glucose for 5 days. After incubation, 103, 104 and 105mU/l of insulin were also added to the each well and incubated for 2 days, respectively. Then, MTT assay and collagen synthesis assay were carried out.

The results indicate that cellular activity of gingival fibroblasts significantly increased by glucose while periodontal ligament cells were unaffected and cellular activity of gingival fibroblasts and periodontal ligament cells were unaffected by insulin.

Collagen synthesis of gingival fibroblast with 20mM glucose and insulin unaffected, but 50mM glucose and insulin increased than control. Collagen synthesis of periodontal ligament cell with 20mM glucose and 105mU/l insulin significantly increased than other groups and 50mM glucose pretreated PDL cells significantly increased at 103mU/l insulin but decreased at 104mU/l insulin.

Our findings indicated that these cell types differed in their growth response to glucose, and the increase in collagen synthesis was significantly raised at insulin level of 103mU/l in gingival fibroblasts and periodontal ligament cells except 20mM glucose pretreated periodontal ligament cells.