ISAba15 Inserted into Outer Membrane Protein Gene carO in Acinetobacter baumannii

Dae Hun Kim; Kwan Soo Ko

doi:10.4167/jbv.2015.45.1.51

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Kim and Ko: ISAba15 Inserted into Outer Membrane Protein Gene carO in Acinetobacter baumannii

Communication

J Bacteriol Virol 2015;45(1):51-53.

Published online: 9 February 2015

DOI: https://doi.org/10.4167/jbv.2015.45.1.51

ISAba15 Inserted into Outer Membrane Protein Gene carO in Acinetobacter baumannii

Dae Hun Kim, Kwan Soo Ko^*

Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Korea

^*Corresponding author: Kwan Soo Ko, Ph.D. Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, 300 Chunchun-dong, Jangan-gu, Suwon 440-746, Korea. Phone: +82-31-299-6223, Fax: +82-31-299-6229, e-mail: ksko@skku.edu

Received 16 December 201431 December 2014 Accepted 7 January 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We identified ISAba15 inserted into the carO gene in an Acinetobacter baumannii isolate. The insert disrupted the lpxD gene, resulting in colistin resistance in A. baumannii. Persistence in carbapenem resistance in A. baumannii isolates with an intact carO gene indicates that loss of the encoded CarO may play a minor role in carbapenem resistance.

Keywords: Carbapenem, Insertion sequence (IS), Antimicrobial resistance

REFERENCES

1). Lee JY, Ko KS. Antimicrobial resistance and clones of Acinetobacter species and Pseudomonas aeruginosa. J Bacteriol Virol. 2012; 42:1–8.

2). Mussi MA, Limansky AS, Aiale AM. Acquisition of resistance to carbapenems in multidrug-resistant clinical strains of Acinetobacter baumannii: natural insertional inactivation of a gene encoding a member of a novel family of β-barrel outer membrane proteins. Antimicrob Agents Chemother. 2005; 49:1432–40.

3). Lee Y, Kim CK, Lee H, Jeong SH, Yong D, Lee K. A novel insertion sequence, ISAba10, inserted into ISAba1 adjacent to the bla(OXA-23) gene and disrupting the outer membrane protein gene carO in Acinetobacter baumannii. Antimicrob Agents Chemother. 2011; 55:361–3.

4). Moffat JH, Harper M, Harrison P, Hale JD, Vinogradov E, Seemann T, et al. Colistin resistance in Acinetobacter baumannii is mediated by complete loss of lipopolysaccharide production. Antimicrob Agents Chemother. 2010; 54:4971–7.

5). Clinical and Laboratory Standards Institute: Performance standards for antimicrobial susceptibility testing; approved guideline. CLSI Document M100-S23. Clinical and Laboratory Standards Institute;Wayne, PA: 2013.

6). Park YK, Jung SI, Park KH, Kim SH, Ko KS. Characteristics of carbapenem-resistant Acinetobacter spp. other than Acinetobacter baumannii in Korea. Int J Antimicrob Agents. 2012; 39:81–5.

Figure 1.

Schematic representation of the genetic organization of ISAba15 interrupting the carO gene in A. baumannii isolate 07AC-017. IRR, right inverted repeats; IRL, left inverted repeats; DR, direct repleat.

Figure 2.

CarO expression levels in two ST92 A. baumannii isolates. While 07AC-007 is an isolate with intact carO gene, 07AC-017 had carO gene interrupted by ISAba15. mRNA levels were normalized to RpoB expression levels.

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