The percent agglutination versus the con A concentration revealed a sigmoid curve in all cases, but the steepness of the sigmoid curve is variable depending on the cell types. It varies even with the same cell but in different species.

Optimum cell concentration was (0.92-0.95) × 10⁷ cells/ml final concentration in the hanging drop, for rabbit erythrocytes, (0.77-1.64) × 10⁷ cells/ml for rat erythrocytes, (1.59-2.7) × 10⁷ cells/ml for human erythrocytes and (0.23-0.39) × 10⁷ cells/ml for sarcoma 180 mouse ascites cells.

When minimal and maximal agglutination percentages were defined as the concentration of con A/ml/1 × 10⁶ cells corresponding to 10% and 95% agglutination, minimal and maximal agglutination occured at 0.56 µg, 19.98 µg for human erythrocytes at 0.56 µg, 224 µg for rat erythrocytes at 0.08 µg, 1.43µg for rabbit erythrocytes at 0.12 µg, 14.8 µg for sarcoma 180-mouse ascites cells respectively.

The order of inhibitory activity of α-methyl-D-mannopyranoside (αMM) for each corresponding cells from the highest inhibition was human erythrocytes, rat erythrocytes, sarcoma 180 mouse ascites cells and rabbit erythrocytes. The concentrations of αMM required for 50% inhibition per ml of the final concentration in the hanging drop per 1 × 10⁷ cells were 0.565 umoles for rabbit erythrocytes, 0.072 umoles for rat erythrocytes, 0.018 umoles for human erythrocytes and 3.677 umoles for sarcoma 180 mouse ascites cells, respectively.

From our experimental results we conclude that the cytoagglutination activity was increased with con A, the inhibitory activity with αMM in the presence of con A was decreased, however the sarcoma 180 mouse ascites cells revealed a contradictory result, and might be due to the topological distribution of agglutination site changes to a distribution more favorable for agglutination.