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Koo, Kang, Park, Lee, Kang, Chae, and Lee: Association of RNase3 Polymorphisms with the Susceptibility of Gastric Cancer
Original Article
Journal of the Korean Surgical Society

Journal of the Korean Surgical Society 2010; 78(5): 283-289.

Published online: 11 May 2010

DOI: https://doi.org/10.4174/jkss.2010.78.5.283

Association of RNase3 Polymorphisms with the Susceptibility of Gastric Cancer

Ja Wook Koo, M.D., Dong Baek Kang, M.D., Won Cheol Park, M.D., Young Hwan Lee, M.D.1, In Hong Kang, M.S.2, Soo Cheon Chae, Ph.D.2, Jeong Kyun Lee, M.D.

Department of Surgery, Institute of Medical Science, College of Medicine, Wonkwang University, Iksan, Korea.

1Department of Radiology, Institute of Medical Science, College of Medicine, Wonkwang University, Iksan, Korea.

2Department of Pathology, Institute of Medical Science, College of Medicine, Wonkwang University, Iksan, Korea.

Corresponding author (rjk@wonkwang.ac.kr)

Received 3 November 2009       Accepted 25 January 2010

Copyright © 2010 The Korean Surgical Society

Abstract

Purpose

RNase3 is a secretory ribonuclease, which is found in the eosinophilic leukocyte and involved in the innate immune system. Its cytotoxic activity is effective against a wide range of pathogens. We performed a case-control study to examine the relationship between RNase3 polymorphisms and the susceptibility of gastric cancer in Korean people.

Methods

Blood sampling of stomach cancer and healthy persons groups were performed, Taqman in g.-550A>G, polymerase chain reaction-restriction fragment length polymorphism in g.371C>G, and high-resolution melt in g.499C>G were analyzed. The three single nucleotide polymorphisms g.-550A>G, g.371C>G, and g.499C>G in RNase3 and their haplotypes were analyzed.

Results

The genotype and allele frequencies of RNase3 g.-550A>G and g.371C>G were not significantly increased in susceptibility of gastric cancer than control group. But, RNase3 CC genotype was associated with a significantly increased susceptibility of gastric cancer than control group (P=0.002). Also, RNase3 CC genotype was more specifically associated with a significantly increased susceptibility of middle and lower gastric cancer than upper gastric cancer (P=0.002). In haplotype of RNase3 SNP g.-550A, g.371G, and g.499C, there was significantly susceptibility of gastric cancer (P=0.004), and more specific influence on middle and lower gastric cancer than upper gastric cancer (P=0.006 vs 0.054).

Conclusion

RNase3 g.499C>G polymorphism may influence gastric cancers, and have a more specific influence on middle and lower gastric cancer rather than upper gastric cancer. But RNase3 g.-550A>G, g.371C>G polymorphisms need careful interpretation and confirmation in more larger studies.

Keywords: RNase3, Polymorphism, Gastric cancer

Figures and Tables

Fig. 1
RNase3 g.371C>G genotyping RFLP result. After restriction enzyme digestion, the PCR products for g.371C>G (1,249 bp) took the form of two fragments, of 1,050 bp and 199 bp.
jkss-78-283-g001
Table 1
Genotype and allele frequencies of the RNase3 polymorphisms between gastric cancer case and control with the susceptibility of gastric cancer
jkss-78-283-i001

*Calculated from the translation start site; †GC = gastric cancer.

Table 2
Genotype and allele frequencies of the RNase3 polymorphisms among upper gastric cancer, middle and lower gastric cancer cases, and control with the susceptibility of gastric cancer
jkss-78-283-i002

*Calculated from the translation start site; †U = upper gastric cancer; ‡M and L = middle and lower gastric cancer; §Values were analyzed between control and upper gastric cancer, and control and middle and lower gastric cancer by Chi-square test.

Table 3
Haplotype frequencies of the RNase3 polymorphisms between gastric cancer case and control with the susceptibility of gastric cancer
jkss-78-283-i003

*Values were constructed using the expectation maximization algorithm with genotyped SNPs. †Values were analyzed by permutation test; ‡GC = gastric cancer.

Table 4
Haplotype frequencies of the RNase3 polymorphisms among upper gastric cancer, middle and lower gastric cancer cases, and control with the susceptibility of gastric cancer
jkss-78-283-i004

*Values were constructed using the expectation maximization algorithm with genotyped SNPs; †Values were analyzed between control and upper gastric cancer, and control and middle and lower gastric cancer by permutation test; ‡U = upper gastric cancer; §M and L = middle and lower gastric cancer.

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