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Abstract
Purpose
The National Cancer Institute (NCI)'s Hollow Fiber Assay (HFA) is currently used as an in vivo screening model to quantitatively define anticancer activity. To investigate the use of HFA in a bladder cancer model, we conducted in vitro and in vivo experiments with several anticancer drugs in nude mice.
Materials and Methods
The human bladder cancer cell lines (CRL2742, 253JP, SW1710, HTB9) were cultured both in vitro and in vivo in polyvinylidene fluoride (PVDF) hollow fibers. The fibers were implanted intraperitoneally (ip) and subcutaneously (sc) into female athymic nude mice (C57BL/6), and the mice were then treated with gemcitabine 120 mg/kg (bolus), cisplatin (3mg/kg), paclitaxel (15mg/kg) or vehicle only (control) for 4-consecutive days. After 6 days, the fibers were retrieved and the viable cell density was analyzed by MTT assay.
Results
The difference between in vitro and in vivo growth was not significant for the CRL2742, 253J-P and SW1710 cell lines; the difference between the ip and sc fibers was also not significant in the CRL2742, SW1710 and HTB9 cell lines. After drug treatment, the percent of growth inhibition revealed constant and effective anticancer activities for the 3 individual drugs.
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Fig. 1.
Implanted sc fibers (arrow) in a nude mouse (ip fibers are not shown). sc: subcutaneous, ip: intraperitoneal.
Fig. 2.
The differences between in vitro and in vivo (ip＋sc) fibers in each of the bladder cancer cell lines. Three of four the cell lines (CRL2742, 253J-P, SW1710) except the HTB9 cell line, reveal no significant differences between the in vitro and in vivo fibers. The difference between the ip and sc fibers was also not significant, except for the 253J-P cell line. ip: intraperitoneal, sc: subcutaneous.
Fig. 3.
Percent (%) of growth inhibition (decease of MTT absorbance) after drug (cisplatin, paclitaxel, gemcitabine) injection for each of the cell lines. (A) % growth inhibition for the in vivo (ip＋sc) fibers, (B) % growth inhibition for the in ip and sc fibers. ip: intraperitoneal, sc: subcutaneous, Cis: cisplatin, Tax: paclitaxel, Gem: gemcitabine.
Table 1.
The MTT absorbance of the in vitro (upper) fibers (18 fibers per each cell line) and the in vivo (lower) intraperitoneal (ip) and subcutaneous (sc) fibers (8 fibers per each cell line)
Table 2.
The MTT absorbance after drug exposure (cisplatin 3mg/kg, paclitaxel 15mg/kg or gemcitabine 120mg/kg bolus) at 4-days (1-day for gemcitabine) after intraperitoneal injection. The control group received vehicle (normal saline) injection only. The individual growth inhibition % for each drug (lower)
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