Clinical Significance of Autoantibodies to Glucose-6-phosphate Isomerase in Serum of Patients with Rheumatoid Arthritis

Yu-Mi Byeon; Sang-Hyon Kim; Sung-Hwan Park

doi:10.4078/jkra.2007.14.4.340

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Byeon, Kim, and Park: Clinical Significance of Autoantibodies to Glucose-6-phosphate Isomerase in Serum of Patients with Rheumatoid Arthritis

Original Article

J Korean Rheum Assoc 2007;14(4):340-344.

Published online: 23 December 2007

DOI: https://doi.org/10.4078/jkra.2007.14.4.340

Clinical Significance of Autoantibodies to Glucose-6-phosphate Isomerase in Serum of Patients with Rheumatoid Arthritis

Yu-Mi Byeon, MD., Sang-Hyon Kim, M D., Sung-Hwan Park, M.D.^∗

Department of Internal Medicine, Chosun University College of Medicine, Gwanju, Korea

^∗Department of Internal Medicine, The Catholic University of Korea School of Medicine, Seoul, Korea

Received 9 October 200719 October 2007

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objecti

Anti-glucose-6-phosphate isomerase (GPI) antibody (Ab) is known to be arthrito- genic in K/BxN mice. Anti-GPI Ab is present in some patients with rheumatoid arthritis (RA), but their clinical manifestations are not clearly elucidated. The purpose of this study was to evaluate whether GPI serves as a specific autoantigen in patients with RA and to investigate the relationship of anti-GPI Ab with clinical parameters of RA.

Methods:

Sera were collected from 54 patients with RA, 15 patients with osteoarthritis (OA) and 28 healthy controls. The samples were tested by enzyme-linked immunosorbent assay (ELISA) using human recombinant GPI as antigen. Patients with RA were classified according to rheumatoid factor (RF) positivity, the presence of RA shared epitope (SE), the presence of extraarticular manifestations, and evidence of bony erosive changes.

Results

Serum levels of anti-GPI Ab were higher in patients with RA than controls (1599.46±1022.48 versus 344.82±223.16 AU, p<0.001), and the levels of patients with OA were also higher than controls (1161.47±917.44 versus 344.82±223.16 AU, p<0.01). In RA, there were no significant difference in anti-GPI Ab levels according to RF positivity, the presence of RA SE, the presence of extraarticular manifestations, and evidence of bony erosive changes.

Conclusion

Our results suggest that anti-GPI Ab may not be RA specific Ab and not related to the severity of RA.

Keywords: Anti-GPI antibody, Rheumatoid arthritis, Shared epitope

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Fig. 1.

Antibodies to glucose-6-phosphate-isomerase (GPI) in serum of healthy controls, patients with rheumatoid arthritis (RA), and with osteoarthritis (OA) by enzyme linked immunosorbent assay (NS∗: not significant).

Fig. 2.

In rheumatoid arthritis, there were no significant difference in serum anti-glucose-6-phosphate-iso- merase (GPI) antibody levels according to rheumatoid factor (RF) positivity, evidence of bony erosive changes, the presence of extraarticular manifestations, and HLA-DR4 positivity. NS∗: not significant, EAM∗∗: extraarticular manifestations.

Fig. 3.

In patients with rheumatoid arthritis (RA), there were no correlations between serum anti-glucose-6- phosphate-isomerase (GPI) antibody levels and erythrocyte sedimentation rate (ESR) or C-reactive protein (CRP). NS: no significance.

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