J Nutr Health JNH Journal of Nutrition and Health 2288-3886 2288-3959 The Korean Nutrition Society 10.4163/jnh.2016.49.6.401 Research Article Four months of magnetized water supplementation improves glycemic control, antioxidant status, and cellualr DNA damage in db/db mice Lee Hye-Jin Kang Myung-Hee Department of Food & Nutrition, Daedeok Valley Campus, Hannam University, 1646 Yuseongdaero, Yuseong-ku, Daejeon 34124, Korea. To whom correspondence should be addressed. tel: +82-42-629-8791, mhkang@hnu.kr 12 2016 31 12 2016 49 6 401 410 17 11 2016 11 12 2016 15 12 2016 © 2016 The Korean Nutrition Society 2016 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Purpose

Water is magnetically charged upon contact with a magnet. Although magnetic water products have been promoted since the 1930's, they have not received wide acceptance since their effectiveness is still in question; however, some have reported their therapeutic effects on the body, especially the digestive, nervous, and urinary systems.

 Methods

In this study, the effect of magnetized water on glycemic control of 14 diabetic mice (CB57BK/KsJ-db/db) in comparison with 10 control mice (CB57BK/KsJ-db/+(db/+)) was investigated. Seven diabetic control (DMC) mice and seven diabetic mice + magnetized water (DM+MW) were kept for 16 weeks, followed by intraperitoneal glucose tolerance test (IPGTT). Weekly blood glucose was measured from tail veins. Blood obtained from heart puncture was used for HbA1c analysis.

 Results

Blood glucose level showed a significant difference starting from the 10th week of study (496.1 ± 10.2 mg/dl in DMC vs. 437.9 ± 76.9 mg/dl in DM+MW). Blood glucose followed by IPGTT showed no significant difference between groups at 0, 30, 60, 90, and 120 min, although glucose level at 180 min was significantly reduced in DM+MW mice. Plasma insulin level in DM+MW groups was only 39.5% of that of DMC groups (5.97 ± 1.69 ng/ml in DMC vs. 2.36 ± 0.94 ng/ml in DM+MW). Levels of HbA1c were 12.4% and 9.7% in DMC and DM+MW groups, respectively.

 Conclusion

These results show the promising therapeutic effect of magnetized water in regulating blood glucose homeostasis; however, long-term supplementation or mechanistic study is necessary.

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310 315 Effect of magnetized water on blood glucose in <italic>db/db</italic> mice. Mean ± SD. Abbreviations: CON, control (n = 10); DMC, dia betes mellitus control (n = 7); DM+MW, DM+Magnetic water (n = 7). Values within a group with different alphabet are significantly different at p < 0.05 by Duncan's multiple range test. Effect of magnetized water on area under the blood glucose curve after oral glucose tolerance test in <italic>db/db</italic> mice. Mean ± SD. Abbreviations: CON, control (n = 10); DMC, diabetes mellitus control (n = 7); DM+MW, DM+Magnetic water (n = 7); AUC, area under the curve. Values with different alphabet are significantly different at p < 0.05 by Duncan's multiple range test. Effect of magnetized water on plasma insulin in <italic>db/db</italic> mice. Mean ± SD. Abbreviations: CON, control (n = 10); DMC, diabetes mellitus control (n = 7); DM+MW, DM+Magnetic water (n = 7). Bar with different letters are significantly different at p < 0.05 after Duncan’s multiple range test. Effect of magnetized water on blood HbA1c in <italic>db/db</italic> mice Mean ± SD. Abbreviations: CON, control (n = 10); DMC, diabetes mellitus control (n = 7); DM+MW, DM+Magnetic water (n = 7). Bar with different letters are significantly different at p < 0.05 after Duncan’s multiple range test. Protective effect of magnetized water on blood DNA damages in <italic>db/db</italic> mice. Mean ± SD. Abbreviations: CON, control (n = 10); DMC, diabetes mellitus control (n = 7); DM+MW, DM+Magnetic water (n = 7). Bar with different letters are significantly different at p < 0.05 after Duncan’s multiple range test. Protective effect of magnetized water on liver DNA damages in <italic>db/db</italic> mice. Mean ± SD. Abbreviations: CON, control (n = 10); DMC, diabetes mellitus control (n = 7); DM+MW, DM+Magnetic water (n = 7). Bar with different letters are significantly different at p < 0.05 after Duncan’s multiple range test. Composition of experimental diet<xref ref-type="bibr" rid="B1">1)</xref>

1) Reeves PG, Nielsen FH, Fahey GC Jr. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr. 123(11):1939-51. 1993 2) AIN-93 diet, Dyets Inc., USA

 Body weight gain, food intake and water intake of mice

Mean ± SD

Abbreviations: CON, control (n = 10); DMC, diabetes mellitus control (n = 7); DM+MW, DM+Magnetic water (n = 7)

Significantly different from control group (p < 0.05) by Duncan's test.

 Effect of magnetized water on plasma TRAP and erythrocyte antioxidant enzymes in <italic>db/db</italic> mice

Mean ± SD

Abbreviations: CON, control (n = 10); DMC, diabetes mellitus control (n = 7); DM+MW, DM+Magnetic water (n = 7)